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Structured illumination microscopy (SIM) reconstructs a super-resolved image from multiple raw images captured with different illumination patterns; hence, acquisition speed is limited, making it unsuitable for dynamic scenes. We propose a…

Optics · Physics 2025-06-17 Ruiming Cao , Fanglin Linda Liu , Li-Hao Yeh , Laura Waller

Structured Illumination Microscopy (SIM) is an imaging technique for achieving both super-resolution (SR) and optical sectioning (OS) in wide-field microscopy. It consists in illuminating the sample with periodic patterns at different…

Optical super-resolution microscopy is a key technology for structural biology that offers high imaging contrast and live-cell compatibility. Minimal (fluorescence) photons flux microscopy, or MINFLUX, is an emerging super-resolution…

Optics · Physics 2025-08-29 Lukas Scheiderer , Zach Marin , Jonas Ries

Multimodal and multi-information microscopy techniques such as Fluorescence Lifetime Imaging Microscopy (FLIM) extend the informational channels beyond intensity-based fluorescence microscopy but suffer from reduced image quality due to…

Image and Video Processing · Electrical Eng. & Systems 2025-04-15 Hao Chen , Julian Najera , Dagmawit Geresu , Meenal Datta , Cody Smith , Scott Howard

Ovarian cancer detection has traditionally relied on a multi-step process that includes biopsy, tissue staining, and morphological analysis by experienced pathologists. While widely practiced, this conventional approach suffers from several…

Super-resolution imaging using sub-diffraction field localization by micron sized transparent beads (microspheres) was recently demonstrated [1]. Practical applications in microscopy require control over the positioning of the microspheres.…

Optics · Physics 2013-12-25 Leonid A. Krivitsky , Jia Jun Wang , Zengbo Wang , Boris Lukiyanchuk

Microwave impedance microscopy (MIM) is a near-field imaging technique that has been used to visualize the local conductivity of materials with nanoscale resolution across the GHz regime. In recent years, MIM has shown great promise for the…

Applied Physics · Physics 2023-10-03 Leonard Weihao Cao , Chen Wu , Rajarshi Bhattacharyya , Ruolun Zhang , Monica T. Allen

Confocal microscopy has long been a cornerstone technique for visualizing complex interactions and processes within cellular structures. However, achieving super-resolution imaging of multiple organelles and their interactions…

Optics · Physics 2025-08-19 Qinglin Chen , Luwei Wang , Jia Li , Dan Shao , Xiaoyu Weng , Liwei Liu , Dayong Jin , Junle Qu

The usage of a GaAsP (gallium arsenide phosphide) photomultiplier for microscopical imaging allows the evaluation of low-light luminescent objects. We designed a setup for collecting a confocal microscopic image signal, which is divided…

Mass spectrometry imaging (MSI) as an analytical tool for bio-molecular and bio-medical research targets, accurate compound localization and identification. In terms of dedicated instrumentation, this translates into the demand for more…

Instrumentation and Detectors · Physics 2013-05-24 Julia H. Jungmann , Ron M. A. Heeren

Behavioural differences across organisms, whether healthy or pathological, are closely tied to the structure of their neural circuits. Yet, the fine-scale synaptic changes that give rise to these variations remain poorly understood, in part…

Computer Vision and Pattern Recognition · Computer Science 2025-09-23 Samia Mohinta , Daniel Franco-Barranco , Shi Yan Lee , Albert Cardona

This paper is concerned with investigating super-resolution algorithms and solutions for handling electron microscopic images. We note two main aspects differentiating the problem discussed here from those considered in the literature. The…

Image and Video Processing · Electrical Eng. & Systems 2020-08-26 Yanjun Qian , Jiaxi Xu , Lawrence F. Drummy , Yu Ding

We demonstrate the combination of a hemispherical solid immersion lens with a micro-photoluminescence setup. Two advantages introduced by the SIL, an improved resolution of 0.4 times the wavelength in vacuum and a 5 times enhancement of the…

Optics · Physics 2009-11-07 S. Moehl , Hui Zhao , B. Dal Don , S. Wachter , H. Kalt

Microorganism enumeration is an essential task in many applications, such as assessing contamination levels or ensuring health standards when evaluating surface cleanliness. However, it's traditionally performed by human-supervised methods…

Computer Vision and Pattern Recognition · Computer Science 2024-12-04 Javier Ureña Santiago , Thomas Ströhle , Antonio Rodríguez-Sánchez , Ruth Breu

Advancement in mid-infrared (MIR) technology has led to promising biomedical applications of MIR spectroscopy, such as liquid biopsy or breath diagnosis. On the contrary, MIR microscopy has been rarely used for live biological samples in an…

High-resolution fMRI provides a window into the brain's mesoscale organization. Yet, higher spatial resolution increases scan times, to compensate for the low signal and contrast-to-noise ratio. This work introduces a deep learning-based 3D…

Image and Video Processing · Electrical Eng. & Systems 2024-03-20 Hongwei Bran Li , Matthew S. Rosen , Shahin Nasr , Juan Eugenio Iglesias

The lack of rapid antibiotic susceptibility tests adversely affects the treatment of bacterial infections and contributes to increased prevalence of multidrug resistant bacteria. Here, we describe an all-electrical approach that allows for…

Applied Physics · Physics 2020-05-06 Yichao Yang , Kalpana Gupta , Kamil L. Ekinci

Sea ice harbours a rich community of well-adapted microorganisms that inhabit liquid micro-spaces where extreme conditions prevail. Currently at risk under climate change, the sea-ice microbiome holds mysteries about evolution of life on…

Atmospheric and Oceanic Physics · Physics 2026-01-13 Béatrice Lessard-Hamel , Marcel Babin , Simon Thibault

Subwavelength imaging by microsphere lenses is a promising label-free super-resolution imaging technique. There is a growing interest to use live cells to replace the widely used non-biological microsphere lenses. In this work, we…

Optics · Physics 2021-07-06 Chunlei Jiang , Hangyu Yue , Bing Yan , Taiji Dong , Xiangyu Cui , Zengbo Wang

Light sheet fluorescence microscopy (LSFM) has transformed the way we visualize biological tissues in three dimensions, offering high-resolution imaging while minimizing photo-induced damage to the samples. Recent breakthroughs in…

Optics · Physics 2025-05-27 Alireza Tabatabaei Mashayekh , Jeremy Witzens