Fast whole-brain imaging of seizures in zebrafish larvae by two-photon light-sheet microscopy
Abstract
Light-sheet fluorescence microscopy (LSFM) enables real-time whole-brain functional imaging in zebrafish larvae. Conventional one photon LSFM can however induce undesirable visual stimulation due to the use of visible excitation light. The use of two-photon (2P) excitation, employing near-infrared invisible light, provides unbiased investigation of neuronal circuit dynamics. However, due to the low efficiency of the 2P absorption process, the imaging speed of this technique is typically limited by the signal-to-noise-ratio. Here, we describe a 2P LSFM setup designed for non-invasive imaging that enables quintuplicating state-of-the-art volumetric acquisition rate of the larval zebrafish brain (5 Hz) while keeping low the laser intensity on the specimen. We applied our system to the study of pharmacologically-induced acute seizures, characterizing the spatial-temporal dynamics of pathological activity and describing for the first time the appearance of caudo-rostral ictal waves (CRIWs).
Keywords
Cite
@article{arxiv.2012.09660,
title = {Fast whole-brain imaging of seizures in zebrafish larvae by two-photon light-sheet microscopy},
author = {Giuseppe de Vito and Lapo Turrini and Caroline Müllenbroich and Pietro Ricci and Giuseppe Sancataldo and Giacomo Mazzamuto and Natascia Tiso and Leonardo Sacconi and Duccio Fanelli and Ludovico Silvestri and Francesco Vanzi and Francesco Saverio Pavone},
journal= {arXiv preprint arXiv:2012.09660},
year = {2021}
}
Comments
Replacement: accepted version of the manuscript, to be published in Biomedical Optics Express. 36 pages, 15 figures