An integrated single- and two-photon non-diffracting light-sheet microscope
Abstract
We describe the apparatus of a fluorescence optical microscope with both single-photon and two-photon non-diffracting light sheets excitation for large volume imaging. With special design to accommodate two different wavelength ranges (visible: 400-700 nm, and near infrared: 800-1200 nm), we combine the line-Bessel sheet (LBS, for single-photon excitation) and the scanning Bessel beam (SBB, for two-photon excitation) light sheet together in a single microscope setup. For a transparent thin sample where the scattering can be ignored, the LBS single-photon excitation is the optimal imaging solution. When the light scattering becomes significant for a deep-cell or deep-tissue imaging, we use SBB light-sheet two-photon excitation with a longer wavelength. We achieved nearly identical lateral/axial resolution of about 350/270 nm for both imagings. This integrated light-sheet microscope may have a wide application for live-cell and live-tissue three-dimensional high-speed imaging.
Keywords
Cite
@article{arxiv.1712.02521,
title = {An integrated single- and two-photon non-diffracting light-sheet microscope},
author = {Sze Cheung Lau and Hoi Chun Chiu and Luwei Zhao and Teng Zhao and M. M. T. Loy and Shengwang Du},
journal= {arXiv preprint arXiv:1712.02521},
year = {2018}
}
Comments
4 pages, 5 figures