Light-sheet microscopy with attenuation-compensated propagation-invariant beams
Abstract
Scattering and absorption limit the penetration of optical fields into tissue, but wavefront correction, often used to compensate for these effects, is incompatible with wide field-of-view imaging and complex to implement. We demonstrate a new approach for increased penetration in light-sheet imaging, namely attenuation-compensation of the light field. This tailors an exponential intensity increase along the illuminating propagation-invariant field, enabling the redistribution of intensity strategically within a sample. This powerful yet straightforward concept, combined with the self-healing of the propagation-invariant field, improves the signal-to-background ratio of Airy light-sheet microscopy up to five-fold and the contrast-to-noise ratio up to eight-fold in thick biological specimens across the field-of-view without any aberration-correction. This improvement is not limited to Airy beam light-sheet microscopy, but can also significantly increase the imaging capabilities of Bessel and lattice light-sheet microscopy techniques, paving the way for widespread uptake by the biomedical community.
Cite
@article{arxiv.1708.02612,
title = {Light-sheet microscopy with attenuation-compensated propagation-invariant beams},
author = {Jonathan Nylk and Kaley McCluskey and Miguel A. Preciado and Michael Mazilu and Frank J. Gunn-Moore and Sanya Aggarwal and Javier A. Tello and David E. K. Ferrier and Kishan Dholakia},
journal= {arXiv preprint arXiv:1708.02612},
year = {2017}
}
Comments
15 pages, 5 figures, supplementary information (35 pages, 22 figures)