A versatile dual spot laser scanning confocal microscopy system for advanced fluorescence correlation spectroscopy analysis in living cell
Optics
2009-08-26 v1
Abstract
A fluorescence correlation spectroscopy (FCS) system based on two independent measurement volumes is presented. The optical setup and data acquisition hardware are detailed, as well as a complete protocol to control the location, size and shape of the measurement volumes. A method that allows to monitor independently the excitation and collection efficiency distribution is proposed. Finally, a few examples of measurements that exploit the two spots in static and/or scanning schemes, are reported.
Keywords
Cite
@article{arxiv.0907.5157,
title = {A versatile dual spot laser scanning confocal microscopy system for advanced fluorescence correlation spectroscopy analysis in living cell},
author = {P. Ferrand and M. Pianta and A. Kress and A. Aillaud and H. Rigneault and D. Marguet},
journal= {arXiv preprint arXiv:0907.5157},
year = {2009}
}
Comments
Accepted for publication in Review of Scientific Instrument