Widefield two-photon excitation without scanning: live cell microscopy with high time resolution and low photo-bleaching
Biological Physics
2015-01-05 v1 Optics
Abstract
We demonstrate fluorescence imaging by two-photon excitation without scanning in biological specimens as previously described by Hwang and co-workers, but with an increased field size and with framing rates of up to 100 Hz. During recordings of synaptically-driven Ca events in primary rat neurone cultures loaded with the fluorescent Ca indicator Fluo-4 AM, we have observed greatly reduced photo-bleaching in comparison with single-photon excitation. This method, which requires no costly additions to the microscope, promises to be useful for work where high time-resolution is required.
Keywords
Cite
@article{arxiv.1501.00044,
title = {Widefield two-photon excitation without scanning: live cell microscopy with high time resolution and low photo-bleaching},
author = {Rumelo Amor and Johanna Trägårdh and Gillian Robb and Louise Wilson and Nor Zaihana Abdul Rahman and John Dempster and William Bradshaw Amos and Trevor J. Bushell and Gail McConnell},
journal= {arXiv preprint arXiv:1501.00044},
year = {2015}
}
Comments
20 pages, 5 figures