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Two-step excitation of fluorescent proteins with real intermediary states

Optics 2025-03-25 v1
Authors: J. David Wong-Campos , Dalia P. Ornelas-Huerta , Mackenzie Dion
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Abstract

We demonstrate sequential two-photon fluorescence microscopy using forbidden state transitions. Nonlinear red excitation leads to green fluorescence in live cells expressing eYFP, maintaining optical sectioning and allowing deep tissue imaging with simple optical systems.

Keywords

fluorescent proteins two-photon microscopy forbidden transitions deep tissue imaging optical sectioning

Cite

@article{arxiv.2503.18000,
  title  = {Two-step excitation of fluorescent proteins with real intermediary states},
  author = {J. David Wong-Campos and Dalia P. Ornelas-Huerta and Mackenzie Dion},
  journal= {arXiv preprint arXiv:2503.18000},
  year   = {2025}
}

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