Spectro-temporal encoded Multiphoton Microscopy
Abstract
Two-Photon Microscopy has become an invaluable tool for biological and medical research, providing high sensitivity, molecular specificity, inherent three-dimensional sub-cellular resolution and deep tissue penetration. In terms of imaging speeds, however, mechanical scanners still limit the acquisition rates to typically 10-100 frames per second. Here we present a high-speed non-linear microscope achieving kilohertz frame rates by employing pulse-modulated, rapidly wavelength-swept lasers and inertia-free beam steering through angular dispersion. In combination with a high bandwidth, single-photon sensitive detector, we achieve recording of fluorescent lifetimes at unprecedented speeds of 88 million pixels per second. We show diffraction-limited, multi-modal, Two-Photon fluorescence and fluorescence lifetime (FLIM), microscopy and imaging flow cytometry with a digitally reconfigurable laser, imaging system and data acquisition system. These unprecedented speeds should enable high-speed and high-throughput image-assisted cell sorting.
Cite
@article{arxiv.1709.00512,
title = {Spectro-temporal encoded Multiphoton Microscopy},
author = {Sebastian Karpf and Carson Riche and Dino di Carlo and Anubhuti Goel and William A. Zeiger and Anand Suresh and Carlos Portera-Cailliau and Bahram Jalali},
journal= {arXiv preprint arXiv:1709.00512},
year = {2020}
}