Continuous focal translation enhances rate of point-scan volumetric microscopy
Abstract
Two-Photon Laser-Scanning Microscopy is a powerful tool for exploring biological structure and function because of its ability to optically section through a sample with a tight focus. While it is possible to obtain 3D image stacks by moving a stage, this perframe imaging process is time consuming. Here, we present a method for an easy to implement and inexpensive modification of an existing two-photon microscope to rapidly image in 3D using an electrically tunable lens to create a tessellating scan pattern which repeats with the volume rate. Using appropriate interpolating algorithms, the volumetric imaging rate can be increased by a factor up to four-fold. This capability provides the expansion of the two-photon microscope into the third dimension for faster volumetric imaging capable of visualizing dynamics on timescales not achievable by traditional stage stack methods.
Keywords
Cite
@article{arxiv.1909.12893,
title = {Continuous focal translation enhances rate of point-scan volumetric microscopy},
author = {Courtney Johnson and Jack Exell and Jonathon Kuo and Kevin Welsher},
journal= {arXiv preprint arXiv:1909.12893},
year = {2020}
}