Fast Wide-field Light Sheet Electro-optic FLIM
Optics
2026-05-06 v2 Applied Physics
Abstract
We demonstrate volumetric fluorescence lifetime microscopy (FLIM) using the electro-optic FLIM technique. Images acquired in a selective plane illumination microscope are gated using a Pockels cell driven at 80 MHz, enabling light sheet FLIM acquisition with up to 800 {\mu}m field of view. Volume acquisitions are demonstrated on fluorescent bead mixtures and in live Arabidopsis thaliana root samples using both genetically encoded fluorescent proteins and endogenous autofluorescence.
Cite
@article{arxiv.2501.15012,
title = {Fast Wide-field Light Sheet Electro-optic FLIM},
author = {V. Rose Knight and Nils Bode and Dara P. Dowlatshahi and Jung-Gun Kim and Mary Beth Mudgett and Soichi Wakatsuki and Adam J. Bowman and Mark A. Kasevich},
journal= {arXiv preprint arXiv:2501.15012},
year = {2026}
}