Full Field Supercritical Angle Fluorescence Microscopy for live cell imaging
Optics
2013-07-22 v1
Abstract
We introduce a full field fluorescence imaging technique with axial confinement of about 100 nm at the sample/substrate interface. Contrary to standard surface imaging techniques, this confinement is obtained through emission filtering. This technique is based on supercritical emission selectivity. It can be implemented on any epifluorescence microscope with a commercial high numerical aperture objective and offers a real time surface imaging capability. This technique is of particular interest for live cell membrane and adhesion studies. Using HEK cells, we show that one can observe simultaneously the surface and in-depth cell phenomena.
Cite
@article{arxiv.1307.5136,
title = {Full Field Supercritical Angle Fluorescence Microscopy for live cell imaging},
author = {Thomas Barroca and Karla Balaa and Julie Delahaye and Sandrine Lévêque-Fort and Emmanuel Fort},
journal= {arXiv preprint arXiv:1307.5136},
year = {2013}
}