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The transmission electron microscope (TEM) has become an essential tool for innovation in nanoscience, material science, and biology. Despite these instruments being widely used across both industry and academia, academics may hesitate to…

Instrumentation and Detectors · Physics 2022-12-07 Patrick McBean , Zachary Milne , Arjun Kanthawar , Cameron O'Byrne , Khalid Hattar , Lewys Jones

Luminescence imaging is invaluable for studying biological and material systems, particularly when advanced protocols that exploit temporal dynamics are employed. However, implementing such protocols often requires custom instrumentation,…

Instrumentation and Detectors · Physics 2025-09-16 Ian Coghill , Alienor Lahlou , Andrea Lodetti , Shizue Matsubara , Johann Boucle , Thomas Le Saux , Ludovic Jullien

Fluorescence imaging is indispensable to biology and neuroscience. The need for large-scale imaging in freely behaving animals has further driven the development in miniaturized microscopes (miniscopes). However, conventional microscopes /…

Optics · Physics 2020-10-29 Yujia Xue , Ian G. Davison , David A. Boas , Lei Tian

Automated and computerised control of scientific instrumentation is almost ubiquitous in the modern laboratory. Most instrumentation is controlled over decades old communication busses or is accessed via proprietary system libraries. This…

Instrumentation and Detectors · Physics 2022-01-07 Joel T. Collins , Joe Knapper , Julian Stirling , Samuel McDermott , Richard Bowman

Correlative microscopy is a powerful technique that combines the advantages of multiple imaging modalities to achieve a comprehensive understanding of investigated samples. For example, fluorescence microscopy provides unique functional…

We describe an inexpensive and accessible instructional setup that explores particle trapping with a planar linear ion trap. The planar trap is constructed using standard printed circuit board manufacturing and is designed to trap…

Analysis of three-dimensional biological samples is critical to understanding tissue function and the mechanisms of disease. Many chronic conditions, like neurodegenerative diseases and cancers, correlate with complex tissue changes that…

Biological Physics · Physics 2019-11-06 Jiaming Guo , Camille Artur , Jason L. Eriksen , David Mayerich

Two-Photon Laser-Scanning Microscopy is a powerful tool for exploring biological structure and function because of its ability to optically section through a sample with a tight focus. While it is possible to obtain 3D image stacks by…

Biological Physics · Physics 2020-01-08 Courtney Johnson , Jack Exell , Jonathon Kuo , Kevin Welsher

Optical spectroscopy techniques such as differential reflectance and transmittance have proven to be very powerful techniques to study 2D materials. However, a thorough description of the experimental setups needed to carry out these…

Laser scanning microscopy enables the acquisition of 3D data in biomedical research. A fundamental challenge in visualizing 3D data is that common flat-panel displays, being 2D in nature, cannot faithfully reproduce light fields. Recent…

Graphics · Computer Science 2025-05-26 Yong Wan , Holly A. Holman , Charles Hansen

We describe the operation of a free-space confocal optical microscope operated in a dilution refrigerator. The microscope is installed on a cold insertable probe to enable fast sample exchange while the refrigerator is held at low…

Quantum Physics · Physics 2021-03-12 B. J. Lawrie , M. Feldman , C. E. Marvinney , Y. Y. Pai

The investigation of optoelectronic devices based on two-dimensional materials and their heterostructures is a very active area of investigation with both fundamental and applied aspects involved. We present a description of a home-built…

Mesoscale and Nanoscale Physics · Physics 2017-05-05 Christoph Reuter , Riccardo Frisenda , Der-Yuh Lin , Tsung-Shine Ko , David Perez de Lara , Andres Castellanos-Gomez

The present work describes a website designed for remote teaching of optical measurements using lasers. It enables senior undergraduate and postgraduate students to learn theoretical aspects of the subject and also have a means to perform…

Physics Education · Physics 2018-03-02 Veena Singh , Richa Dubey , P. K. Panigrahi , K. Muralidhar

Glass is increasingly desired as a material for manufacturing complex microscopic geometries, from the micro-optics in compact consumer products to microfluidic systems for chemical synthesis and biological analyses. As the size, geometric,…

A simple strobe setup with potential to study higher-order eigenmodes and multifrequency oscillations in micromechanical resonators is described. It requires standard equipment, commonly found in many laboratories, and it can thus be…

Instrumentation and Detectors · Physics 2013-02-13 Andres Castellanos-Gomez

One-shot full-field surface profilometry using digital diffractive-confocal imaging correlation microscope based on digital micromirror device is developed for one-shot microscopic 3D surface measurement. Optical configuration applies…

Optics · Physics 2019-04-24 Duc Trung Nguyen , Liang-Chia Chen , Nguyen Dinh Nguyen

An optical microscope enables image-based findings and diagnosis on microscopic targets, which is indispensable in many scientific, industrial and medical settings. Majority of microscope users are accustomed to standard benchtop…

Optics · Physics 2025-05-06 Keiichiro Toda , Miu Tamamitsu , Ryoichi Horisaki , Takuro Ideguchi

Owing to its capacity for unique (bio)-chemical specificity, microscopy withmid-IR illumination holds tremendous promise for a wide range of biomedical and industrial applications. The primary limitation, however, remains detection; with…

In this paper we propose a low-cost high-speed imaging line scan system. We replace an expensive industrial line scan camera and illumination with a custom-built set-up of cheap off-the-shelf components, yielding a measurement system with…

Computer Vision and Pattern Recognition · Computer Science 2016-12-08 Stef Van Wolputte , Wim Abbeloos , Stijn Helsen , Abdellatif Bey-Temsamani , Toon Goedemé

Reflective imaging systems form an important part of photonic devices such as spectrometers, telescopes, augmented and virtual reality headsets or lithography platforms. Reflective optics provide unparalleled spectral performance and can be…