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Tracking single molecules is instrumental for quantifying the transport of molecules and nanoparticles in biological samples, e.g., in brain drug delivery studies. Existing intensity-based localisation methods are not developed for imaging…

Artificial defects embedded in periodic structures are important foundation for creating localized states with vast range of applications in condensed matter physics, photonics and acoustics. In photonics, localized states are extensively…

Optics · Physics 2018-02-14 Hamidreza Ramezani , Pankaj Jha , Yuan Wang , Xiang Zhang

Single epitaxially-grown semiconductor quantum dots have great potential as single photon sources for photonic quantum technologies, though in practice devices often exhibit non-ideal behavior. Here, we demonstrate that amplitude modulation…

Quantum Physics · Physics 2013-03-08 Serkan Ates , Imad Agha , Angelo Gulinatti , Ivan Rech , Antonio Badolato , Kartik Srinivasan

Intensity modulators are an essential component in optics for controlling free-space beams. Many applications require the intensity of a free-space beam to be modulated at a single frequency, including wide-field lock-in detection for…

The spontaneous fluorescence rates of single-molecule emitters are typically on the order of nanoseconds. However coupling them with plasmonic nanostructures can substantially increase their fluorescence yields. The confinement between the…

Mesoscale and Nanoscale Physics · Physics 2024-02-01 Jiří Doležal , Amandeep Sagwal , Rodrigo Cezar de Campos Ferreira , Martin Švec

Measuring subdiffraction separations between single fluorescent particles is important for biological, nano-, and medical-technology studies. Major challenges include (i) measuring changing molecular separations with high temporal…

Biological Physics · Physics 2015-05-18 Shawn H. DeCenzo , Michael C. DeSantis , Y. M. Wang

Colocalization is a powerful tool to study the interactions between fluorescently labeled molecules in biological fluorescence microscopy. However, existing techniques for colocalization analysis have not undergone continued development…

Methodology · Statistics 2018-02-14 Shulei Wang , Ellen T. Arena , Kevin W. Eliceiri , Ming Yuan

We present a rapid-scanning approach to fluorescence-detected two-dimensional electronic spectroscopy that combines acousto-optic phase-modulation with digital lock-in detection. The approach shifts the signal detection window to suppress…

We prove that it is impossible to distinguish two spatially coinciding fluorescent molecules from a single rotating molecule using polarization-sensitive imaging, even if one modulates the polarization of the illumination or the detection…

Optics · Physics 2025-03-11 Yiyang Chen , Yuanxin Qiu , Matthew D. Lew

The use of photo-activated fluorescent molecules to create long sequences of low emitter-density diffraction-limited images enables high-precision emitter localization, but at the cost of low temporal resolution. We suggest combining…

Image and Video Processing · Electrical Eng. & Systems 2023-07-19 Gili Dardikman-Yoffe , Yonina C. Eldar

We developed a time-resolved force microscopy technique by integrating atomic force microscopy using a tuning-fork-type cantilever with the delay time modulation method for optical pump-probe light. We successfully measured the dynamics of…

The coupling of nanostructures with emitters opens ways for the realization of man-made subwavelength light emitting elements. In this article, we investigate the modification of fluorescence when an emitter is placed close to a…

Other Condensed Matter · Physics 2009-11-13 S. Kuehn , G. Mori , M. Agio , V. Sandoghdar

Time-resolved photoluminescence (PL) is commonly used to track dynamics in a broad range of materials. Thus, the search for simplification of the acquisition of PL kinetics attracts continuous attention. This paper presents a new robust and…

Instrumentation and Detectors · Physics 2020-04-22 Jiri Junek , Lukas Ondic , Karel Zidek

We present a method for calibrating the response of a phase-only spatial light modulator in nonlinear microscopy. Our method uses the microscope image itself as calibration measurement and requires no additional hardware components. Our…

Optics · Physics 2025-09-04 Daniël W. S. Cox , Harish Sasikumar , Ivo M. Vellekoop

We exploit memory effect speckle correlations for the imaging of incoherent linear (single-photon) fluorescent sources behind scattering tissue. While memory effect-based imaging techniques have been heavily studied in the past, for thick…

We present an ultra-fast, precise, parameter-free method, which we term Deep-STORM, for obtaining super-resolution images from stochastically-blinking emitters, such as fluorescent molecules used for localization microscopy. Deep-STORM uses…

Optics · Physics 2018-05-03 Elias Nehme , Lucien E. Weiss , Tomer Michaeli , Yoav Shechtman

We report, for the first time, a multi-confocal Fluorescence Correlation Spectroscopy (mFCS) technique which allows parallel measurements at different locations, by combining a Spatial Light Modulator (SLM), with an Electron Multiplying-CCD…

Multifocal microscopy (MFM) offers high-speed three-dimensional imaging through the simultaneous image capture from multiple focal planes. Conventional MFM systems use a fabricated grating in the emission path for a single emission…

Biological Physics · Physics 2019-07-31 M. Junaid Amin , Sabine Petry , Haw Yang , Joshua W. Shaevitz

Super-resolution ultrasound imaging through microbubble (MB) localisation and tracking, also known as ultrasound localisation microscopy, allows non-invasive sub-diffraction resolution imaging of microvasculature in animals and humans. The…

Image and Video Processing · Electrical Eng. & Systems 2025-05-23 Su Yan , Clotilde Vié , Marcelo Lerendegui , Herman Verinaz-Jadan , Jipeng Yan , Martina Tashkova , James Burn , Bingxue Wang , Gary Frost , Kevin G. Murphy , Meng-Xing Tang

Accurate characterization using static light scattering (SLS) and dynamic light scattering (DLS) methods mandates the measurement and analysis of singly-scattered light. In turbid samples, the suppression of multiple scattering is therefore…

Soft Condensed Matter · Physics 2012-09-18 Ian Block , Frank Scheffold