Related papers: Time-modulated excitation for enhanced single mole…
Achieving localization with molecular precision has been of great interest for extending fluorescence microscopy to nanoscopy. MINFLUX pioneers this transition through point spread function (PSF) engineering, yet its performance is…
Among the approaches in three-dimensional (3D) single molecule localization microscopy, there are several point spread function (PSF) engineering approaches, in which depth information of molecules is encoded in 2D images. Usually,the…
We present a novel experimental setup in which magnetic and optical tweezers are combined for torque and force transduction onto single filamentous molecules in a transverse configuration to allow simultaneous mechanical measurement and…
We introduce MINFLUX, a concept for localizing photon emitters in space. By probing the emitter with a local intensity minimum of excitation light, MINFLUX minimizes the fluorescence photons needed for high localization precision. A 22-fold…
Confocal microscopy of fluorescent labeled particles has been used to study the dynamical and structural properties of colloidal and granular matter in real space. Localization algorithms allow for a fully automatized determination of the…
In wide-field nonlinear microscopy, wavefront modulation by means of phase-only spatial light modulators (SLMs) allows achieving simultaneous two-photon excitation and fluorescence emission from specific region-of-interests (ROIs) of…
Recently, the spectral manipulation of single photons has been achieved through spatial-temporal modulation of the optical refractive index. Here, we generalize this mechanism to massive particles, i.e. realizing the acceleration or…
Precisely measuring the three-dimensional position and orientation of individual fluorophores is challenging due to the substantial photon shot noise in single-molecule experiments. Facing this limited photon budget, numerous techniques…
The concepts of topology provide a powerful tool to tailor the propagation and localization of light. While electromagnetic waves have only two polarization states, engineered degeneracies of photonic modes provide novel opportunities…
Single-molecule localization fluorescence microscopy constructs super-resolution images by sequential imaging and computational localization of sparsely activated fluorophores. Accurate and efficient fluorophore localization algorithms are…
The diffraction of light imposes a fundamental limit on the resolution of light microscopes. This limit can be circumvented by creating and exploiting independent behaviors of the sample at length scales below the diffraction limit. In…
Lightwave-driven scanning tunnelling microscopy (STM) at near-IR frequencies promises an unprecedented combination of atomic spatial resolution and temporal resolution approaching the attosecond range. To achieve this goal, high-sensitivity…
Recent technological advances in cutting-edge ultrasensitive fluorescence microscopy have allowed single-molecule imaging experiments in living cells across all three domains of life to become commonplace. Single-molecule live-cell data is…
A novel single-sideband (SSB) time-modulated technique with 2-bit phase shifters is proposed. The timemodulated module is implemented by adding periodic phase modulation to 2-bit phase shifters, which is simpler without performance loss…
Acquiring precise information about the mode content of a laser is critical for multiplexed optical communications, optical imaging with active wave-front control, and quantum-limited interferometric measurements. Hologram-based mode…
A time-of-flight (ToF) imaging system is proposed and its working principle demonstrated. To realize this system, a new device, a free-space optical mixer, is designed and fabricated. A scene is illuminated (flashed) with a megahertz level…
We demonstrate a significant resolution enhancement beyond the conventional limit in multiphoton microscopy (MPM) using saturated excitation of fluorescence. Our technique achieves super-resolved imaging by temporally modulating the…
Single-molecule localization microscopy (SMLM) allows reconstructing biology-relevant structures beyond the diffraction limit by detecting and localizing individual fluorophores -- fluorescent molecules stained onto the observed specimen --…
The use of fluorescent molecules to create long sequences of low-density, diffraction-limited images enables highly-precise molecule localization. However, this methodology requires lengthy imaging times, which limits the ability to view…
Three-dimension real-time tracking of single emitters is an emerging tool for assessment of biological behavior as intraneuronal transport, for which spatiotemporal resolution is crucial to understand the microscopic interactions between…