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Standard deviation measurements of intensity profiles of stationary single fluorescent molecules are useful for studying axial localization, molecular orientation, and a fluorescence imaging system's spatial resolution. Here we report on…

Quantitative Methods · Quantitative Biology 2015-05-13 Michael C. DeSantis , Shawn H. DeCenzo , Je-Luen Li , Y. M. Wang

Colocalization aims at characterizing spatial associations between two fluorescently-tagged biomolecules by quantifying the co-occurrence and correlation between the two channels acquired in fluorescence microscopy. Colocalization is…

Applications · Statistics 2019-07-12 Frédéric Lavancier , Thierry Pécot , Liu Zengzhen , Charles Kervrann

We consider ramifications of the use of high speed light modulators to questions of correlation and measurement of time-energy entangled photons. Using phase modulators, we find that temporal modulation of one photon of an entangled pair,…

Quantum Physics · Physics 2009-11-13 S. E. Harris

The temporal modulation of material parameters enables optical amplification within linear media. Here we consider the fundamental building block of plasmonics, a subwavelength metal nanoparticle, and study how temporal modulation alters…

Mesoscale and Nanoscale Physics · Physics 2025-08-29 Miguel Verde , Paloma A. Huidobro

We investigate theoretically how single-molecule spectroscopy techniques can be used to perform fast and high resolution displacement detection and manipulation of nanomechanical oscillators, such as singly clamped carbon nanotubes. We…

Mesoscale and Nanoscale Physics · Physics 2015-10-21 Vadim Puller , Brahim Lounis , Fabio Pistolesi

In single molecule localisation super-resolution microscopy the need for repeated image capture limits the imaging speed, while the size of fluorescence probes limits the possible theoretical localisation resolution. Here, we demonstrated a…

We introduce a superresolution technique that combines spatial mode demultiplexing (SPADE) with emitter blinking. We show that temporal fluctuations not only enhance the precision of SPADE imaging, but also drastically simplify the…

Optics · Physics 2025-11-27 Stanislaw Kurdzialek

Single-pixel imaging, originally developed in light optics, facilitates fast three-dimensional sample reconstruction, as well as probing with light wavelengths undetectable by conventional multi-pixel detectors. However, the spatial…

FRET-based approaches are a unique tool for sensing the immediate surroundings and interactions of (bio)molecules. FRET imaging and FLIM (Fluorescence Lifetime Imaging Microscopy) enable the visualization of the spatial distribution of…

Localization microscopy is an imaging technique in which the positions of individual nanoscale point emitters (e.g. fluorescent molecules) are determined at high precision from their images. This is the key ingredient in…

Image and Video Processing · Electrical Eng. & Systems 2020-10-01 Elias Nehme , Daniel Freedman , Racheli Gordon , Boris Ferdman , Lucien E. Weiss , Onit Alalouf , Reut Orange , Tomer Michaeli , Yoav Shechtman

Optical detection and spectroscopy of single molecules has become an indispensable tool in biological imaging and sensing. Its success is based on fluorescence of organic dye molecules under carefully engineered laser illumination. In this…

Chemical Physics · Physics 2011-01-18 Ilja Gerhardt , Lijian Mai , Antia Lamas-Linares , Christian Kurtsiefer

Multimode squeezed light is a key resource for high-dimensional quantum technologies, enhancing metrological sensitivity, boosting communication security, and enabling parallel processing in computation. Its practical potential, however,…

Quantum Physics · Physics 2025-10-09 Mahmoud Kalash , Aditya Sudharsanam , M. H. M. Passos , Valentina Parigi , Maria Chekhova

Spatio-temporally modulated impedance surfaces can be good candidates for generation of radiating waves with arbitrary eigenstates by breaking momentum and energy conservations. Here, we present a theoretical framework based on the…

Applied Physics · Physics 2021-12-16 Amrollah Amini , Homayoon Oraizi

3D super-resolution fluorescence microscopy typically requires sophisticated setups, sample preparation, or long measurements. A notable exception, SOFI, only requires recording a sequence of frames and no hardware modifications whatsoever…

Optics · Physics 2024-03-01 Pawel Szczypkowski , Monika Pawlowska , Radek Lapkiewicz

We present a new two-snapshot structured light illumination (SLI) reconstruction algorithm for fast image acquisition. The new algorithm, which only requires two mutually {\pi} phase-shifted raw structured images, is implemented on a…

Optics · Physics 2017-10-25 Yunlong Meng , Wei Lin , Chenglin Li , Shih-chi Chen

We describe an experimental system that allows one to easily access the dispersion curve of exciton-polaritons in a microcavity. Our approach is based on two spatial light modulators (SLM), one for changing the excitation angles (momenta),…

Mesoscale and Nanoscale Physics · Physics 2015-06-03 P. Mai , B. Pressl , M. Sassermann , Z. Vörös , G. Weihs , C. Schneider , A. Löffler , S. Höfling , A. Forchel

Apparatus for fluorescence-based single photon generation includes collection optics and various setups for characterization. Managing this system often reveals complexity in such a way that adjusting in a small region changes optimal…

Optics · Physics 2019-11-19 Hee-Jin Lim , Kwang-Yong Jeong , Dong-Hoon Lee , Kee Suk Hong

Single dye molecules at cryogenic temperatures display many spectroscopic phenomena known from free atoms and are thus promising candidates for fundamental quantum optical studies. However, the existing techniques for the detection of…

Quantum Physics · Physics 2008-02-12 Gert Wrigge , Ilja Gerhardt , Jaesuk Hwang , Gert Zumofen , Vahid Sandoghdar

Single molecule localization microscopy (SMLM) permits the visualization of cellular structures an order of magnitude smaller than the diffraction limit of visible light, and an accurate, objective evaluation of the resolution of an SMLM…

LiDAR relocalization aims to estimate the global 6-DoF pose of a sensor in the environment. However, existing regression-based approaches are prone to dynamic or ambiguous scenarios, as they either solely rely on single-frame inference or…

Computer Vision and Pattern Recognition · Computer Science 2026-02-04 Minghang Zhu , Zhijing Wang , Yuxin Guo , Wen Li , Sheng Ao , Cheng Wang