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Fast 3D super-resolution imaging is essential for decoding rapidly occurring biological processes. Encoding single molecules to their respective planes enable simultaneous multi-plane super-resolution volume imaging. This saves the…

Biological Physics · Physics 2016-08-04 Partha Pratim Mondal

In this paper, we consider the problem in defocus image deblurring. Previous classical methods follow two-steps approaches, i.e., first defocus map estimation and then the non-blind deblurring. In the era of deep learning, some researchers…

Computer Vision and Pattern Recognition · Computer Science 2022-07-08 Qian Ye , Masanori Suganuma , Takayuki Okatani

Fluorescent molecules are versatile nanoscale emitters that enable detailed observations of biophysical processes with nanoscale resolution. Because they are well-approximated as electric dipoles, imaging systems can be designed to…

Optics · Physics 2024-03-26 Tingting Wu , Matthew D. Lew

This work presents new experimental results on low-temperature (LT) characterization of local rf properties of passive superconducting (SC) microwave devices using a novel Laser Scanning Microscope (LSM). In this technique, a modulated…

Superconductivity · Physics 2016-11-18 A. P. Zhuravel , A. V. Ustinov , D. Abraimov , Steven M. Anlage

Single-molecule microscopy has become an indispensable tool for biochemical analysis. The capability of characterizing distinct properties of individual molecules without averaging has provided us with a different perspective for the…

Applied Physics · Physics 2020-05-21 Miao-Hsuan Chien , Silvan Schmid

Structured illumination microscopy (SIM) uses a set of images captured with different illumination patterns to computationally reconstruct resolution beyond the diffraction limit. Here, we propose an alternative approach using a single…

Optics · Physics 2025-06-17 Ruiming Cao , Guanghan Meng , Laura Waller

Existing super-resolution microscopy is often constrained by inherent trade-offs between resolution, acquisition speed, phototoxicity, and hardware complexity. Computational post-processing approaches offer a promising alternative, but they…

A new technique based on superposition of two speckle patterns is proposed and demonstrated for controlled modulation of the spatial polarization distribution of the resultant speckle. It is demonstrated both theoretically and…

Optics · Physics 2021-11-15 Abhijit Roy , Rakesh K. Singh , Maruthi M. Brundavanam

Diffuse optical imaging (DOI) offers valuable insights into scattering mediums, but the quest for high-resolution imaging often requires dense sampling strategies, leading to higher imaging errors and lengthy acquisition times. This work…

Optics · Physics 2025-04-07 Ben Wiesel , Shlomi Arnon

Cells sense external concentrations and, via biochemical signaling, respond by regulating the expression of target proteins. Both in signaling networks and gene regulation there are two main mechanisms by which the concentration can be…

Subcellular Processes · Quantitative Biology 2017-04-12 Gabriele Micali , Gerardo Aquino , David M. Richards , Robert G. Endres

We propose an enhanced spatial modulation (SM)-based scheme for indoor visible light communication systems. This scheme enhances the achievable throughput of conventional SM schemes by transmitting higher order complex modulation symbol,…

Signal Processing · Electrical Eng. & Systems 2022-01-21 Shimaa Naser , Lina Bariah , Sami Muhaidat , Mahmoud Al-Qutayri , Paschalis C. Sofotasios

Time synchronization and phase shaping of single photons both play fundamental roles in quantum information applications that rely on multi-photon quantum interference. Phase shaping typically requires separate modulators with extra…

Quantum Physics · Physics 2021-09-14 Keyu Su , Yunfei Wang , Shanchao Zhang , Zhuoping Kong , Yi Zhong , Jianfeng Li , Hui Yan , Shi-Liang Zhu

Spatial Fluorescence Cross Correlation Spectroscopy is a rarely investigated version of Fluorescence Correlation Spectroscopy, in which the fluorescence signals from different observation volumes are cross-correlated. In the reported…

Optics · Physics 2008-06-13 Yoann Blancquaert , Jie Gao , Jacques Derouard , Antoine Delon

Molecular fluorescence microscopy is a leading approach to super-resolution and nanoscale imaging in life and material sciences. However, super-resolution fluorescence microscopy is often bottlenecked by system-specific calibrations and…

We consider a microscopy setting where quantum light is used for illumination. Spontaneous parametric down conversion (SPDC) is used as a source of a heralded single photon, which is quantum light prepared in a Fock state. We present…

Quantum Physics · Physics 2023-06-08 Maria Gieysztor , Joshua Nepinak , Christopher J. Pugh , Piotr Kolenderski

We investigate experimentally the nonlocal phase modulation of multiple-frequency-mode, continuous-variable entangled twin beams. We use a pair of electro-optical phase modulators to modulate the entangled probe and conjugate light beams…

Dedicated systems are fundamental for neuroscience experimental protocols that require timing determinism and synchronous stimuli generation. We developed a data acquisition and stimuli generator system for neuroscience research, optimized…

Quantitative Methods · Quantitative Biology 2015-04-08 Paulo Matias , Rafael Tuma Guariento , Lirio Onofre Baptista de Almeida , Jan Frans Willem Slaets

In single-molecule super-resolution microscopy, engineered point-spread functions (PSFs) are designed to efficiently encode new molecular properties, such as 3D orientation, into complex spatial features captured by a camera. To fully…

Image and Video Processing · Electrical Eng. & Systems 2019-07-16 Hesam Mazidi , Eshan S. King , Oumeng Zhang , Arye Nehorai , Matthew D. Lew

We implement a super-localization method in the time domain that allows the observation of the external motion of soliton molecules in a fiber ring cavity laser with unprecedented accuracy. In particular, we demonstrate the synchronization…

Pattern Formation and Solitons · Physics 2022-06-08 Saïd Hamdi , Aurélien Coillet , Benoit Cluzel , Philippe Grelu , Pierre Colman

Speckled illumination enhances widefield fluorescence microscopy by enabling optical sectioning and super resolution. In random illumination microscopy, sequences of speckled illumination patterns are used to excite fluorescent samples and…

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