English

Inline calibration of spatial light modulators in nonlinear microscopy

Optics 2025-09-04 v1

Abstract

We present a method for calibrating the response of a phase-only spatial light modulator in nonlinear microscopy. Our method uses the microscope image itself as calibration measurement and requires no additional hardware components. Our method is adapted to the nonlinear signals encountered in multi-photon excitation fluorescence microscopes, and works well even under low light conditions and with strong photobleaching.

Keywords

Cite

@article{arxiv.2505.22482,
  title  = {Inline calibration of spatial light modulators in nonlinear microscopy},
  author = {Daniël W. S. Cox and Harish Sasikumar and Ivo M. Vellekoop},
  journal= {arXiv preprint arXiv:2505.22482},
  year   = {2025}
}

Comments

10 pages, 5 figures

R2 v1 2026-07-01T02:46:39.884Z