Phase modulated multiphoton microscopy
Optics
2015-03-12 v1
Abstract
We show that the modulation of the phases of the laser beams of ultra-short pulses leads to modulation of the two photon fluorescence intensity. The phase modulation technique when used in multi-photon microscopy can improve the signal to noise ratio. The technique can also be used in multiplexing the signals in the frequency domain in multi-focal raster scanning microscopy. As the technique avoids the use of array detectors as well as elaborate spatiotemporal multiplexing schemes it provides a convenient means to multi-focal scanning in axial direction. We show examples of such uses. Similar methodology can be used in other non-linear scanning microscopies, such as second or third harmonic generation microscopy.
Cite
@article{arxiv.1503.03386,
title = {Phase modulated multiphoton microscopy},
author = {Khadga Jung Karki and Mohammed Abdellah and Tonu Pullerits},
journal= {arXiv preprint arXiv:1503.03386},
year = {2015}
}