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Correlative microscopy is a powerful technique that combines the advantages of multiple imaging modalities to achieve a comprehensive understanding of investigated samples. For example, fluorescence microscopy provides unique functional…

The current state of the art in structural biology is led by NMR, X-ray crystallography and TEM investigations. These powerful tools however all rely on averaging over a large ensemble of molecules. Here, we present an alternative concept…

Atomic and Molecular Clusters · Physics 2015-12-15 Tatiana Latychevskaia , Jean-Nicolas Longchamp , Conrad Escher , Hans-Werner Fink

Diffuse Reflectance Spectroscopy (DRS) is a well-established optical technique for tissue composition assessment which has been clinically evaluated for tumour detection to ensure the complete removal of cancerous tissue. While point-wise…

Robotics · Computer Science 2025-03-12 Kaizhong Deng , Christopher J. Peters , George P. Mylonas , Daniel S. Elson

Accurate relative spectrophotometry is critical for many science applications. Small wavelength scale residuals in the flux calibration can significantly impact the measurements of weak emission and absorption features in the spectra. Using…

Instrumentation and Methods for Astrophysics · Physics 2011-10-14 Renbin Yan

Near-infrared (NIR) detectors -- which use non-destructive readouts to measure time-series counts-per-pixel -- play a crucial role in modern astrophysics. Standard NIR flux extraction techniques were developed for space-based observations…

Optical fluorescence imaging is capable of measuring both the spatial and rotational dynamics of single molecules. However, unavoidable measurement noise will result in inaccurate estimates of rotational dynamics, causing a molecule to…

Optics · Physics 2019-05-22 Oumeng Zhang , Matthew D. Lew

This paper proposes a novel feature called spectrum congruency for describing edges in images. The spectrum congruency is a generalization of the phase congruency, which depicts how much each Fourier components of the image are congruent in…

Image and Video Processing · Electrical Eng. & Systems 2021-03-11 Fang Yang , Xin Su , Li Chai

Recently, Neural Radiance Fields (NeRF) have emerged as a potent method for synthesizing novel views from a dense set of images. Despite its impressive performance, NeRF is plagued by its necessity for numerous calibrated views and its…

Computer Vision and Pattern Recognition · Computer Science 2023-03-13 Jiayang Bai , Letian Huang , Wen Gong , Jie Guo , Yanwen Guo

Fluorescence microscopy and derived techniques are continuously looking for photodetectors able to guarantee increased sensitivity, high spatial and temporal resolution and ease of integration into modern microscopy architectures. Recent…

Noninvasive, non-contact and \textit{in vivo} monitoring of blood glucose is a long needed pathology tool for saving patients from recurring pain and hassle that can accompany conventional blood glucose testing methods. Optical coherence…

Biological Physics · Physics 2008-08-04 R Poddar , S R Sharma , P Sen , J T Andrews

In this paper, we use steady-state measurements to obtain evidence of radiation trapping in an optically thick a cloud of cold rubidium atoms. We investigate the fluorescence properties of our sample, pumped on opened transitions. The…

Atomic Physics · Physics 2015-06-11 Quentin Baudouin , Nicolas Mercadier , Robin Kaiser

Early detection of retinal diseases is one of the most important means of preventing partial or permanent blindness in patients. In this research, a novel multi-label classification system is proposed for the detection of multiple retinal…

Computer Vision and Pattern Recognition · Computer Science 2022-08-01 M. A. Rodriguez , H. AlMarzouqi , P. Liatsis

Fluorescence microscopy has become a widely used tool for studying various biological structures of in vivo tissue or cells. However, quantitative analysis of these biological structures remains a challenge due to their complexity which is…

Computer Vision and Pattern Recognition · Computer Science 2019-04-16 Soonam Lee , Chichen Fu , Paul Salama , Kenneth W. Dunn , Edward J. Delp

While studying nucleic acids to reveal the weak interactions responsible for their three-dimensional structure and for their interactions with drugs, we also contributed to the field of biomolecular mass spectrometry, both in terms of…

Biological Physics · Physics 2023-06-08 Valérie Gabelica

Microrheology, the study of fluids on micron length-scales, promises to reveal insights into cellular biology, including mechanical biomarkers of disease and the interplay between biomechanics and cellular function. Here a…

In this research, a reflection-based nanohole array sensor system is presented for discriminating between migration-competent cancer cells that maintain the integrity of the actin cortex and those cells lacking the actin cortex and thus…

Optical coherence tomography offers astounding opportunities to image the complex structure of living tissue, but lacks functional information. We present dynamic full-field optical coherence tomography to image living human induced…

Recent developments in stimulated emission depletion (STED) microscopy achieved nanometer scale resolution and showed great potential in live cell imaging. Yet, STED nanoscopy techniques are based on single point-scanning. This constitutes…

Biological Physics · Physics 2015-06-16 Bin Yang , Frédéric Przybilla , Michael Mestre , Jean-Baptiste Trebbia , Brahim Lounis

The thousandfold increase in data-collection speed enabled by aberration-corrected optics allows us to overcome an electron microscopy paradox - how to obtain atomic-resolution chemical structure in individual nanoparticles, yet record a…

The fluorescence of organic fluorophore molecules is enhanced when they are placed in contact with certain metals (Al, Ag, Cu, Au, etc.) whose surface plasmon waves couple into the radiative modes of the molecules and increase the radiative…

Mesoscale and Nanoscale Physics · Physics 2015-04-21 S. Sarkar , B. Kanchibotla , J. D. Nelson , J. D. Edwards , J. Anderson , G. C. Tepper , S. Bandyopadhyay