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Random graph models are used to describe the complex structure of real-world networks in diverse fields of knowledge. Studying their behavior and fitting properties are still critical challenges, that in general, require model specific…

Statistics Theory · Mathematics 2023-08-30 Suzana de Siqueira Santos , André Fujita , Catherine Matias

Single-molecule approaches to biology offer a powerful new vision to elucidate the mechanisms that underpin the functioning of living cells. However, conventional optical single molecule spectroscopy techniques such as F\"orster…

The analysis of the ultrasonic frequency-dependent backscatter coefficient of aggregating red blood cells reveals information about blood structural properties. The difficulty to apply this technique in vivo is due to the…

Biological Physics · Physics 2009-11-13 Emilie Franceschini , François T. H. Yu , Guy Cloutier

Fluorescence microscopy has revolutionized biomedical research over the past three decades. Its high molecular specificity and unrivaled single molecule level sensitivity have enabled breakthroughs in a variety of research fields. For in…

Optics · Physics 2012-03-21 Meng Cui , Ke Si , Reto Fiolka

Spectroscopic investigations provide important insights into the composition of luminescence emissions relevant to trapped-charge dating of sediments. Accurate wavelength calibration and a correction for the wavelength-dependent detection…

Data Analysis, Statistics and Probability · Physics 2024-08-16 Mariana Sontag-González , Dirk Mittelstraß , Sebastian Kreutzer , Markus Fuchs

Diffusion models are a special type of generative model, capable of synthesising new data from a learnt distribution. We introduce DISPR, a diffusion-based model for solving the inverse problem of three-dimensional (3D) cell shape…

Computer Vision and Pattern Recognition · Computer Science 2023-03-15 Dominik J. E. Waibel , Ernst Röell , Bastian Rieck , Raja Giryes , Carsten Marr

We report the cell biological applications of a recently developed multiphoton fluorescence lifetime imaging microscopy system using a streak camera (StreakFLIM). The system was calibrated with standard fluorophore specimens and was shown…

Cell Behavior · Quantitative Biology 2009-11-10 R. V. Krishnan , Eva Biener , Jian-Hua Zhang , Robert Heckel , Brian Herman

We present a method for recovery of narrow homogeneous spectral features out of broad inhomogeneous overlapped profile based on second-derivative processing of the absorption spectra of alkali metal atomic vapor nanocells. The method is…

We perform spectroscopy on the hyperfine splitting of $^{85}$Rb atoms trapped in far-off-resonance optical traps. The existence of a spatially dependent shift in the energy levels is shown to induce an inherent dephasing effect, which…

Atomic Physics · Physics 2016-08-16 A. Kaplan , M. F. Andersen , T. Grünzweig , N. Davidson

Super-resolution microscopy is rapidly gaining importance as an analytical tool in the life sciences. A compelling feature is the ability to label biological units of interest with fluorescent markers in living cells and to observe them…

Protein rotational kinetics are essential for understanding macromolecular behavior in crowded environments, yet measuring these dynamics at solid-liquid interfaces remains a significant challenge due to low signal strengths. Here, we…

Resonance fluorescence arises from the interaction of an optical field with a two-level system and has played a fundamental role in the development of quantum optics and its applications. Despite its conceptual simplicity it entails a wide…

Raman spectroscopy is widely used across scientific domains to characterize the chemical composition of samples in a non-destructive, label-free manner. Many applications entail the unmixing of signals from mixtures of molecular species to…

Ultrawide-field fluorescein angiography (UWF-FA) facilitates diabetic retinopathy (DR) detection by providing a clear visualization of peripheral retinal lesions. However, the intravenous dye injection with potential risks hamper its…

Image and Video Processing · Electrical Eng. & Systems 2026-02-25 Ruoyu Chen , Kezheng Xu , Kangyan Zheng , Weiyi Zhang , Yan Lu , Danli Shi , Mingguang He

Diffusion-weighted magnetic resonance imaging (DW-MRI) is a critical imaging method for capturing and modeling tissue microarchitecture at a millimeter scale. A common practice to model the measured DW-MRI signal is via fiber orientation…

Droplet microfluidics offers a versatile platform for analyzing liquid samples. Despite its potential, there is a lack of techniques that allow to reliably probe individual circulating droplets. The prospect of combining droplet…

Quantitative Methods · Quantitative Biology 2024-03-08 Marc Sulliger , Jaime Ortega Arroyo , Romain Quidant

Increasing demand for understanding the vast heterogeneity of cellular phenotypes has driven the development of imaging flow cytometry (IFC), that combines features of flow cytometry with fluorescence and bright field microscopy. IFC…

Quantitative Methods · Quantitative Biology 2019-02-06 Yuanyuan Han , Rui Tang , Yi Gu , Alex Ce Zhang , Wei Cai , Violet Castor , Sung Hwan Cho , William Alaynick , Yu-Hwa Lo

We have developed an automatic method for segmenting fluorescence lifetime (FLT) imaging microscopy (FLIM) images of cells inspired by a multi-resolution community detection (MCD) based network segmentation method. The image processing…

Medical Physics · Physics 2014-01-06 Dandan Hu , Pinaki Sarder , Peter Ronhovde , Sandra Orthaus , Samuel Achilefu , Zohar Nussinov

Determining the number of fluorescent entities that are coupled to a given molecule (DNA, protein, etc.) is a key point of numerous biological studies, especially those based on a single molecule approach. Reliable methods are important, in…

A new pathway to nuclear magnetic resonance spectroscopy in high pressure diamond anvil cells is introduced, using inductively coupled broadband passive electro-magnetic lenses to locally amplify the magnetic flux at the isolated sample,…

Instrumentation and Detectors · Physics 2017-06-02 Thomas Meier , Nan Wang , Dario Mager , Jan G. Korvink , Sylvain Petigirard , Leonid Dubrovinsky