Related papers: Autofluorescence spectroscopy for determining cell…
Constrictions in blood vessels and microfluidic devices can dramatically change the spatial distribution of passing cells or particles and are commonly used in biomedical cell sorting applications. However, the three-dimensional nature of…
Quantifying the number of molecules from fluorescence microscopy measurements is an important topic in cell biology and medical research. In this work, we present a consecutive algorithm for super-resolution (STED) scanning microscopy that…
Lensless imaging enables exceptionally compact fluorescence sensors, advancing applications in \textit{in vivo} imaging and low-cost, point-of-care diagnostics. These sensors require a filter to block the excitation light while passing…
Cell deformability is an essential determinant for tissue-scale mechanical nature, such as fluidity and rigidity, and is thus crucial for understanding tissue homeostasis and stable developmental processes. However, numerical simulations…
A fluorescence correlation spectroscopy (FCS) system based on two independent measurement volumes is presented. The optical setup and data acquisition hardware are detailed, as well as a complete protocol to control the location, size and…
Nanophotonics achieves accurate control over the luminescence properties of a single quantum emitter by tailoring the light-matter interaction at the nanoscale and modifying the local density of optical states (LDOS). This paradigm could…
The health of cells found in the inner retinal layers of the human eye is crucial to understand the onset of diseases of the retina such as macular degeneration and retinopathy. A challenge is to periodically image these cells in human eyes…
We establish a method, called pbFFS for photobleaching Fluctuation Fluorescence Spectroscopy, which aims at characterizing molecules or particles labelled with a unknown distribution or fluorophores. Using photobleaching as a control…
The analysis of different image modalities is frequently performed in ophthalmology as it provides complementary information for the diagnosis and follow-up of relevant diseases, like hypertension or diabetes. This work presents a hybrid…
Photobleaching is a major limitation of superresolution Stimulated Depletion Emission (STED) microscopy. Fast scanning has long been considered an effective means to reduce photobleaching in fluorescence microscopy, but a careful…
Focal ratio degradation (FRD), the increase of light's focal ratio between the input into an optical fiber and the output, is important to characterize for astronomical spectrographs due to its effects on throughput and the point spread…
Focal ratio degradation (FRD) is a major contributor to throughput and light loss in a fibre spectroscopic telescope system. We combine the guided mode theory in geometric optics and a well-known model, power distribution model (PDM), to…
We introduce a top-ranked cycle flux ranking scheme of network analysis to assess the performance of molecular junction solar cells. By mapping the Lindblad master equation to the quantum-transition network, we propose a microscopic…
Various techniques have been developed to measure the 2D and 3D positions and 2D and 3D orientations of fluorescent molecules with improved precision over standard epifluorescence microscopes. Due to the challenging signal-to-background…
The olive oil sector produces a substantial impact in the Mediterranean's economy and lifestyle. Many studies exist which try to optimize the different steps in the olive oil's production process. One of the main challenges for olive oil…
When calculating the optical absorption spectra of molecular crystals from first principles, the influence of the crystalline environment on the excitations is of significant importance. For such systems, however, methods to describe the…
Imaging techniques such as functional near-infrared spectroscopy (fNIRS) and diffuse optical tomography (DOT) achieve deep, non-invasive sensing in turbid media, but they are constrained by the photon budget. Wavefront shaping (WFS) can…
Numerical investigation of the interaction of electromagnetic fields with eukaryotic cells requires specifically adapted computer models. Virtual microdosimetry, used to investigate exposure, requires volumetric cell models, which are…
Optical spectroscopy is an important and widely used technique, for instance, to characterize new materials and to identify unknown compounds. Spectra are typically reported as a function of the wavelength of light, yet the information…
Inside living cells are complex mixtures of thousands of components. It is hopeless to try to characterise all the individual interactions in these mixtures. Thus, we develop a statistical approach to approximating them, and examine the…