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Spatial resolution of most imaging devices is fundamentally restricted by diffraction. This limitation is manifested in the loss of high spatial frequency information contained in evanescent waves. As a result, conventional far-field optics…

Optics · Physics 2010-03-16 Leonid Alekseyev , Evgenii Narimanov , Jacob Khurgin

In both light optics and electron optics, the amplitude of a wave scattered by an object is an observable that is usually recorded in the form of an intensity distribution in a real space image or a diffraction image. In contrast, retrieval…

Improving the resolution of fluorescence microscopy beyond the diffraction limit can be achievedby acquiring and processing multiple images of the sample under different illumination conditions.One of the simplest techniques, Random…

In this paper we analyze an imaging technique based on intensity speckle correlations over incident field position proposed in [J. A. Newmann and K. J. Webb, Phys. Rev. Lett. 113, 263903 (2014)]. Its purpose is to reconstruct a field…

Analysis of PDEs · Mathematics 2018-08-01 Josselin Garnier , Knut Solna

Single-pixel imaging has emerged as a key technique in fluorescence microscopy, where fast acquisition and reconstruction are crucial. In this context, images are reconstructed from linearly compressed measurements. In practice, total…

Image and Video Processing · Electrical Eng. & Systems 2025-07-28 Serban C. Tudosie , Valerio Gandolfi , Shivaprasad Varakkoth , Andrea Farina , Cosimo D'Andrea , Simon Arridge

We present an ultra-thin hybrid imaging system based on an optical multimode fiber (MMF) and an optical fiber hydrophone that combines optical resolution photoacoustic and fluorescence microscopy. To control the illumination at the distal…

Diffraction-limited imaging through complex scattering media is a long sought after goal with important applications in biomedical research. In recent years, high resolution wavefront-shaping has emerged as a powerful approach to generate a…

Optics · Physics 2014-10-17 Ori Katz , Eran Small , Yefeng Guan , Yaron Silberberg

Low-light optical imaging refers to the use of cameras to capture images with minimal photon flux. This area has broad application to diverse fields, including optical microscopy for biological studies. In such studies, it is important to…

We introduce a full field fluorescence imaging technique with axial confinement of about 100 nm at the sample/substrate interface. Contrary to standard surface imaging techniques, this confinement is obtained through emission filtering.…

The field of biomedical imaging has undergone a rapid growth in recent years, mostly due to the implementation of ad-hoc designed experimental setups, theoretical support methods and numerical reconstructions. Especially for biological…

Biological Physics · Physics 2017-06-30 Daniele Ancora

Simultaneous imaging of fluorescence-labeled and label-free phase objects in the same sample provides distinct and complementary information. Most multimodal fluorescence-phase imaging operates in transmission mode, capturing fluorescence…

Optics · Physics 2024-08-20 Renzhi He , Yucheng Li , Junjie Chen , Yi Xue

Light scattering and aberrations limit optical microscopy in biological tissue, which motivates the development of adaptive optics techniques. Here, we develop a method for adaptive optics with reflected light and deep neural networks…

Optics · Physics 2020-07-28 Ivan Vishniakou , Johannes D. Seelig

Optical imaging through complex media, such as biological tissues or fog, is challenging due to light scattering. In the multiple scattering regime, wavefront shaping provides an effective method to retrieve information; it relies on…

Light scattering within scattering media presents a substantial obstacle to optical transmission. A speckle pattern with random amplitude and phase distribution is observed when coherent light travels through strong scattering media.…

Optics · Physics 2022-09-01 Hui Liu , Xiangyu Zhu , Xiaoxue Zhang , Xudong Chen , Zhili Lin

Fourier ptychographic microscopy (FPM) is a computational approach geared towards creating high-resolution and large field-of-view images without mechanical scanning. To acquire color images of histology slides, it often requires sequential…

Image and Video Processing · Electrical Eng. & Systems 2020-10-28 Ruihai Wang , Pengming Song , Shaowei Jiang , Chenggang Yan , Jiakai Zhu , Chengfei Guo , Zichao Bian , Tianbo Wang , Guoan Zheng

Imaging inside scattering media at optical resolution is a longstanding challenge affecting multiple fields, from bio-medicine to astronomy. In recent years, several groundbreaking techniques for imaging inside scattering media, in…

Optics · Physics 2024-05-17 Elad Sunray , Gil Weinberg , Moriya Rosenfeld , Ori Katz

Fourier ptychographic (FP) microscope is a coherent imaging method that can synthesize an image with a higher bandwidth using multiple low-bandwidth images captured at different spatial frequency regions. The method's demand for multiple…

Optics · Physics 2016-03-25 Jaebum Chung , Hangwen Lu , Xiaoze Ou , Haojiang Zhou , Changhuei Yang

Achieving fast, large-scale volumetric imaging with micrometer resolution has been a persistent challenge in the field of biological microscopy. To address this challenge, we report an augmented version of light field microscopy,…

Optics · Physics 2024-10-11 Aymerick Bazin , Amaury Badon

Our work aims at using quantitative imaging tools to complement the limitation of noise encountered by high resolution fluorescence microscopy methods. Several cycles of fluorophore activation, imaging and deactivation produce a sequence of…

Quantitative Methods · Quantitative Biology 2012-02-03 Juliane Liepe , Federico Felizzi , Agata Pernus , Maria Hanulova

High-throughput biological imaging is often constrained by a trade-off between acquisition speed and image quality. Fast imaging modalities, such as wide-field fluorescence microscopy, enable large-scale data acquisition but suffer from…

Image and Video Processing · Electrical Eng. & Systems 2026-04-20 Dominik Panek , Carina Rząca , Maksymilian Szczypior , Joanna Sorysz , Krzysztof Misztal , Zbigniew Baster , Zenon Rajfur
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