Related papers: Wide field fluorescence epi-microscopy behind a sc…
Aberrations limit scanning fluorescence microscopy when imaging in scattering materials such as biological tissue. Model-based approaches for adaptive optics take advantage of a computational model of the optical setup. Such models can be…
Volumetric biological imaging often involves compromising high temporal resolution at the expense of high spatial resolution when popular scanning methods are used to capture 3D information. We introduce an integrated experimental and image…
An interferometric fluorescent microscope and a novel theoretic image reconstruction approach were developed and used to obtain super-resolution images of live biological samples and to enable dynamic real time tracking. The tracking…
Optical imaging through scattering media is a fundamental challenge in many applications. Recently, substantial breakthroughs such as imaging through biological tissues and looking around corners have been obtained by the use of…
A new principle of subwavelength imaging based on frequency scanning is considered. It is shown that it is possible to reconstruct the spatial profile of an external field exciting an array (or coupled arrays) of subwavelength-sized…
Fluorescence molecular tomography (FMT) is a sensitive optical imaging technology widely used in biomedical research. However, the ill-posedness of the inverse problem poses a huge challenge to FMT reconstruction. Although end-to-end deep…
Incoherently illuminated or luminescent objects give rise to a low-contrast speckle-like pattern when observed through a thin diffusive medium, as such a medium effectively convolves their shape with a speckle-like point spread function…
Stimulated Emission Depletion (STED) microscopy has emerged as a powerful technique providing visualization of biological structures at the molecular level in living samples. In this technique, the diffraction limit is broken by selectively…
High resolution optical microscopy is essential in neuroscience but suffers from scattering in biological tissues. It therefore grants access to superficial layers only. Recently developed techniques use scattered photons for imaging by…
Light passing through scattering media will be strongly scattered and diffused into complex speckle pattern, which contains almost all the spatial information and spectral information of the objects. Although various methods have been…
Optical phase contains key information for biomedical and astronomical imaging. However, it is often obscured by layers of heterogeneous and scattering media, which render optical phase imaging at different depths an utmost challenge.…
The resolving ability of widefield fluorescence microscopy is fundamentally limited by out-of-focus background owing to its low axial resolution, particularly for densely labeled biological samples. Although total internal reflection…
The measurement of the Transmission Matrix (TM) of a scattering medium is of great interest for imaging. It can be acquired directly by interferometry using an internal reference wavefront. Unfortunately, internal reference fields are…
Traditional fluorescence microscopy is constrained by inherent trade-offs among resolution, field-of-view, and system complexity. To navigate these challenges, we introduce a simple and low-cost computational multi-aperture miniature…
From multi-photon imaging penetrating millimeters deep through scattering biological tissue, to super-resolution imaging conquering the diffraction limit, optical imaging techniques have greatly advanced in recent years. Notwithstanding, a…
Non-invasive optical focusing inside scattering media is still a big challenge because inhomogeneous media scatter both incoming photons for focusing and outgoing photons for observation. Various approaches, utilizing non-linear…
Imaging through scattering is a pervasive and difficult problem in many biological applications. The high background and the exponentially attenuated target signals due to scattering fundamentally limits the imaging depth of fluorescence…
Correlative microscopy is a powerful technique that combines the advantages of multiple imaging modalities to achieve a comprehensive understanding of investigated samples. For example, fluorescence microscopy provides unique functional…
Extended depth of field microscopy encodes axial information into a single acquisition through engineered point spread functions, but conventional and deep optics approaches are subject to degradation in scattering tissue. We introduce…
Paradoxically, imaging with resolution much below the wavelength $\lambda$ - now common place in the visible spectrum - remains challenging at lower frequencies, where arguably it is needed most due to the large wavelengths used. Techniques…