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Optical approaches to fluorescent, spectroscopic, and morphological imaging have made exceptional advances in the last decade. Super-resolution imaging and wide-field multiphoton imaging are now underpinning major advances across the…

Fluorescence microscopy is an important and extensively utilised tool for imaging biological systems. However, the image resolution that can be obtained has a limit as defined through the laws of diffraction. Demand for improved resolution…

Biological Physics · Physics 2007-08-27 James H. Rice

Highly confined vectorial electromagnetic field distributions represent an excellent tool for detailed studies in nano-optics and high resolution microscopy, such as nonlinear microscopy, advanced fluorescence imaging or nanoplasmonics.…

Optics · Physics 2014-07-03 Thomas Bauer , Sergej Orlov , Ulf Peschel , Peter Banzer , Gerd Leuchs

In biological microscopy, light scattering represents the main limitation to image at depth. Recently, a set of wavefront shaping techniques has been developed in order to manipulate coherent light in strongly disordered materials. The…

Optics · Physics 2021-01-07 Antoine Boniface , Jonathan Dong , Sylvain Gigan

Fiber-based endoscopes utilizing multi-core fiber (MCF) bundles offer the capability to image deep within the human body, making them well-suited for imaging applications in minimally invasive surgery or diagnosis. However, the optical…

Imaging through opaque, highly scattering walls is a long sought after capability with potential applications in a variety of fields. The use of wavefront shaping to compensate for scattering has brought a renewed interest as a potential…

Optical diffraction tomography is an indispensable tool for studying objects in three-dimensions due to its ability to accurately reconstruct scattering objects. Until now this technique has been limited to coherent light because spatial…

While scattered light conveys most of the information we perceive, scattering may also distort that information before it reaches our detectors. The problem is acute in many applications, such as in high-resolution microscopy of biological…

For several centuries, far-field optical microscopy has remained a key instrument in many scientific disciplines including physical, chemical and biomedical research. Nonetheless, far-field imaging has many limitations: the spatial…

Optics · Physics 2020-01-27 Lyubov V. Amitonova , Johannes F. de Boer

Human ability to visualize an image is usually hindered by optical scattering. Recent extensive studies have promoted imaging technique through turbid materials to a reality where color image can be restored behind scattering media in real…

Lensless fiber endomicroscope is an emerging tool for in-vivo microscopic imaging, where quantitative phase imaging (QPI) can be utilized as a label-free method to enhance image contrast. However, existing single-shot phase reconstruction…

Wavefront-shaping is a promising approach for imaging fluorescent targets deep inside scattering tissue despite strong aberrations. It enables focusing an incoming illumination into a single spot inside tissue, as well as correcting the…

Optics · Physics 2024-01-23 Dror Aizik , Anat Levin

Mid-infrared photothermal microscopy is a new chemical imaging technology in which a visible beam senses the photothermal effect induced by a pulsed infrared laser. This technology provides infrared spectroscopic information at sub-micron…

Optics · Physics 2021-04-08 Yi Zhang , Haonan Zong , Cheng Zong , Yuying Tan , Meng Zhang , Yuewei Zhan , Ji-Xin Cheng

Fluorescence fluctuations-based super-resolution microscopy (FF-SRM) is an emerging field promising low-cost and live-cell compatible imaging beyond the resolution of conventional optical microscopy. A comprehensive overview on how the…

Optical-resolution fluorescence imaging through and within complex samples presents a significant challenge due to random light scattering, with substantial implications across multiple fields. While significant advancements in coherent…

Optics · Physics 2023-12-27 Gil Weinberg , Elad Sunray , Ori Katz

Deep, high-resolution imaging is essential for unraveling biological complexity and advancing medical diagnostics, yet scattering fundamentally limits optical methods. Among the most promising approaches, photoacoustic imaging achieves…

Subwavelength imaging by microsphere lenses is a promising label-free super-resolution imaging technique. There is a growing interest to use live cells to replace the widely used non-biological microsphere lenses. In this work, we…

Optics · Physics 2021-07-06 Chunlei Jiang , Hangyu Yue , Bing Yan , Taiji Dong , Xiangyu Cui , Zengbo Wang

Optical imaging through scattering media is a commonly confronted with the problem of reconstruction of complex objects and optical memory effect. To solve the problem, here, we propose a novel configuration based on the combination of…

Image and Video Processing · Electrical Eng. & Systems 2020-08-26 Meiling Zhou , An Pan , Runze Li , Yansheng Liang , Junwei Min , Tong Peng , Chen Bai , Baoli Yao

In turbid media, scattering of light scrambles information of the incident beam and represents an obstacle to optical imaging. Noninvasive imaging through opaque layers is challenging for dynamic and wide-field objects due to unreliable…

Optics · Physics 2018-10-17 Yuan Yuan , Hui Chen

A robust method and strategy for efficient full field-ofview and depth separation optical imaging through scattering media regardless of the three-dimensional (3D) optical memory effect are proposed. In this method, the problem of imaging…

Optics · Physics 2020-01-17 Wei Li , Jietao Liu , Shunfu He , Lixian Liu , Xiaopeng Shao