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Related papers: Wide field fluorescence epi-microscopy behind a sc…

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Over the past decade, optical wavefront shaping has been developed to focus light through highly opaque scattering layers, opening new possibilities for biomedical applications. To probe light intensity deep inside soft scattering media…

Optics · Physics 2025-08-25 Fei Xia , Ivo Leite , Robert Prevedel , Thomas Chaigne

We present the experimental reconstruction of sub-wavelength features from the far-field intensity of sparse optical objects: sparsity-based sub-wavelength imaging combined with phase-retrieval. As examples, we demonstrate the recovery of…

We present an optical wavefront shaping approach that allows tracking and localization of signal hidden inside or behind a scattering medium. The method combines traditional feedback based wavefront shaping together with a switch function,…

This paper presents an application of digital Fourier holography for selective imaging of scatterers with different sizes in turbid media such as biological tissues. A combination of Fourier holography and high-resolution digital recording,…

Optics · Physics 2007-05-23 Sergey A. Alexandrov , P. Meredith , T. J. McIntyre , A. V. Zvyagin

Fourier ptychographic microscopy allows for the collection of images with a high space-bandwidth product at the cost of temporal resolution. In Fourier ptychographic microscopy, the light source of a conventional widefield microscope is…

Image and Video Processing · Electrical Eng. & Systems 2019-01-30 Yi Fei Cheng , Megan Strachan , Zachary Weiss , Moniher Deb , Dawn Carone , Vidya Ganapati

The use of photo-activated fluorescent molecules to create long sequences of low emitter-density diffraction-limited images enables high-precision emitter localization, but at the cost of low temporal resolution. We suggest combining…

Image and Video Processing · Electrical Eng. & Systems 2023-07-19 Gili Dardikman-Yoffe , Yonina C. Eldar

Interferometric scattering microscopy is a powerful technique that enables various applications, such as mass photometry and particle tracking. Here we present a numerical toolbox to simulate images obtained in interferometric scattering,…

Single-shot volumetric fluorescence (SVF) imaging offers a significant advantage over traditional imaging methods that require scanning across multiple axial planes as it can capture biological processes with high temporal resolution. The…

Recently, novel imaging techniques based on the 'memory-effect' speckle-correlations have enabled diffraction-limited imaging through scattering layers and around corners. These techniques, however, are currently limited to imaging only…

Optics · Physics 2018-11-14 Ofer Salhov , Gil Weinberg , Ori Katz

Fluorescence microscopy has become a widely used tool for studying various biological structures of in vivo tissue or cells. However, quantitative analysis of these biological structures remains a challenge due to their complexity which is…

Computer Vision and Pattern Recognition · Computer Science 2019-04-16 Soonam Lee , Chichen Fu , Paul Salama , Kenneth W. Dunn , Edward J. Delp

Fluorescence fluctuation spectroscopy is applied to study molecules, passing through a small observation volume, usually subjected to diffusive or convective motion in liquid phase. We suggest that such a technique could be used to measure…

The speckle imaging is a photographic technique that resolves objects viewed through severely distorted media. The results are insensitive to the errors caused by apparent size of the isoplanatic patch and the telescope aberrations. In this…

Astrophysics · Physics 2007-05-23 S K Saha

Label-free microscopy exploits light scattering to obtain a three-dimensional image of biological tissues. However, light propagation is affected by aberrations and multiple scattering, which drastically degrade the image quality and limit…

Image restoration of biological structures in microscopy poses unique challenges for preserving fine textures and sharp edges. While recent GAN-based image restoration formulations have introduced frequency-domain losses for natural images,…

Quantitative Methods · Quantitative Biology 2026-01-30 Xingjian Zhang , Claire Leclech , Louison Blivet-Bailly , Abdul I. Barakat , Elsa D. Angelini

In optical tomography a physical body is illuminated with near-infrared light and the resulting outward photon flux is measured at the object boundary. The goal is to reconstruct internal optical properties of the body, such as absorption…

Numerical Analysis · Mathematics 2016-01-20 Antti Hannukainen , Lauri Harhanen , Nuutti Hyvönen , Helle Majander

As light travels through a disordered medium such as biological tissues, it undergoes multiple scattering events. This phenomenon is detrimental to in-depth optical microscopy, as it causes a drastic degradation of contrast, resolution and…

A new method has been developed for the correction of the distortions and/or enhanced phase differentiation in Electron Backscatter Diffraction (EBSD) data. Using a multi-modal data approach, the method uses segmented images of the phase of…

Data Analysis, Statistics and Probability · Physics 2019-03-11 Marie-Agathe Charpagne , Florian Strub , Tresa M. Pollock

We measured images and Fourier images of fluorescence for 0.11- and 0.22-$\mu$m-diameter dye-doped polystyrene micro-sphere beads on a solid immersion lens, and experimentally verified strongly-angle-dependent fluorescence intensities due…

Instrumentation and Detectors · Physics 2009-11-07 Masahiro Yoshita , Kazuko Koyama , Motoyoshi Baba , Hidefumi Akiyama

Light-field microscopy represents a promising solution for microscopic volumetric imaging, thanks to its capability to encode information on multiple planes in a single acquisition. This is achieved through its peculiar simultaneous capture…

Despite the tremendous progresses in wavefront control through or inside complex scattering media, several limitations prevent reaching practical feasibility for nonlinear imaging in biological tissues. While the optimization of nonlinear…

Optics · Physics 2016-12-06 Hilton B. de Aguiar , Sylvain Gigan , Sophie Brasselet
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