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Structured illumination can reject out-of-focus signal from a sample, enabling high-speed and high-contrast imaging over large areas with widefield detection optics. Currently, this optical-sectioning technique is limited by image…

Single-shot wide-angle diffraction imaging is a widely used method to investigate the structure of non-crystallizing objects such as nanoclusters, large proteins or even viruses. Its main advantage is that information about the…

Data Analysis, Statistics and Probability · Physics 2021-06-02 Thomas Stielow , Stefan Scheel

Imaging through a single optical fiber offers attractive possibilities in many applications such as microendoscopy or remote sensing. However, the direct transmission of an image through an optical fiber is difficult because spatial…

Optics · Physics 2015-05-29 Roman Barankov , Jerome Mertz

Three-photon (3P) microscopy is getting traction due to its superior performance in deep tissues. Yet, aberrations and light scattering still pose one of the main limitations in the attainable depth ranges for high-resolution imaging. Here,…

Diffraction limit is manifested in the loss of high spatial frequency information that results from decay of evanescent waves. As a result, conventional far-field optics yields no information about an object's subwavelength features. Here…

Optics · Physics 2012-01-24 Leonid Alekseyev , Evgenii Narimanov , Jacob Khurgin

The emergence of far-field super-resolution microscopy has rejuvenated the possibility for nanoscale imaging. Approaches to far-field super-resolution that utilize point scanning often depends on spatially reducing the size of the focused…

Optics · Physics 2022-06-17 Ning Xu , Guoxuan Liu , Qiaofeng Tan

Light sheet fluorescence microscopy (LSFM) has transformed the way we visualize biological tissues in three dimensions, offering high-resolution imaging while minimizing photo-induced damage to the samples. Recent breakthroughs in…

Optics · Physics 2025-05-27 Alireza Tabatabaei Mashayekh , Jeremy Witzens

Memory-effect-based methods have been demonstrated to be feasible to observe hidden objects through thin scattering layers, even from a single-shot speckle pattern. However, most of the existing methods are performed with narrowband…

Image and Video Processing · Electrical Eng. & Systems 2018-09-20 Tengfei Wu , Chengfei Guo , Xiaopeng Shao

Fluorescence microscopic imaging in centimeter-deep tissue has been highly sought-after for many years because much interesting in vivo micro-information, such as microcirculation, tumor angiogenesis, and metastasis, may deeply locate in…

Optical spectrometers are widely used scientific equipment with many applications involving material characterization, chemical analysis, disease diagnostics, surveillance, etc. Emerging applications in biomedical and communication fields…

Volumetric imaging of samples using fluorescence microscopy plays an important role in various fields including physical, medical and life sciences. Here we report a deep learning-based volumetric image inference framework that uses 2D…

Optics · Physics 2021-03-24 Luzhe Huang , Yilin Luo , Yair Rivenson , Aydogan Ozcan

Optical spectroscopy is a fundamental tool in numerous areas of science and technology. Much effort has focused on miniaturizing spectrometers, but thus far at the cost of high spectral resolution and broad operating range. Here, we…

Endomicroscopy is an emerging imaging modality, that facilitates the acquisition of in vivo, in situ optical biopsies, assisting diagnostic and potentially therapeutic interventions. While there is a diverse and constantly expanding range…

Computer Vision and Pattern Recognition · Computer Science 2020-01-01 Antonios Perperidis , Kevin Dhaliwal , Stephen McLaughlin , Tom Vercauteren

The back-focal plane (BFP) of a high-numerical aperture objective contains the fluoro-phore radiation pattern, which encodes information about the axial fluorophore position, molecular orientation and the local refractive index of the…

Breaking the diffraction limit in microscopy by utilizing quantum properties of light has been the goal of intense research in the recent years. We propose a quantum superresolution technique based on non-classical emission statistics of…

Optics · Physics 2013-05-30 Osip Schwartz , Dan Oron

Fluorescence Molecular Tomography (FMT) is a promising technique for non-invasive 3D visualization of fluorescent probes, but its reconstruction remains challenging due to the inherent ill-posedness and reliance on inaccurate or…

Image and Video Processing · Electrical Eng. & Systems 2025-11-07 Shihan Zhao , Jianru Zhang , Yanan Wu , Linlin Li , Siyuan Shen , Xingjun Zhu , Guoyan Zheng , Jiahua Jiang , Wuwei Ren

Super-resolution fluorescence microscopy, with a resolution beyond the diffraction limit of light, has become an indispensable tool to directly visualize biological structures in living cells at a nanometer-scale resolution. Despite…

Computer Vision and Pattern Recognition · Computer Science 2018-09-05 Yu Li , Fan Xu , Fa Zhang , Pingyong Xu , Mingshu Zhang , Ming Fan , Lihua Li , Xin Gao , Renmin Han

We propose a new method to image fluorescent objects through turbid media base on Airy beam scanning. This is achieved by using the non-diffractive nature of Airy beams, namely their ability to maintain their shape while penetrating through…

Optics · Physics 2019-05-01 Harel Nagar , Yael Roichman

Speckle based imaging consists of forming a super-resolved reconstruction of an unknown sample from low-resolution images obtained under random inhomogeneous illuminations (speckles). In a blind context where the illuminations are unknown,…

Data Analysis, Statistics and Probability · Physics 2017-11-10 Jérôme Idier , Simon Labouesse , Marc Allain , Penghuan Liu , Sébastien Bourguignon , Anne Sentenac

The knowledge of the exact structure of the optical system PSF enables a high-quality image reconstruction in fluorescence microscopy. Accurate PSF models account for the vector nature of light and the phase and amplitude modifications.…