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Light microscopy as well as image acquisition and processing suffer from physical and technical prejudices which preclude a correct interpretation of biological observations which can be reflected in, e.g., medical and pharmacological…

Quantitative Methods · Quantitative Biology 2019-08-15 Renata Rychtarikova , Georg Steiner , Gero Kramer , Michael B. Fischer , Dalibor Stys

Recently introduced speckle-correlations based techniques enable noninvasive imaging of objects hidden behind scattering layers. In these techniques the hidden object Fourier amplitude is retrieved from the scattered light autocorrelation,…

Optics · Physics 2022-01-19 Tengfei Wu , Ori Katz , Xiaopeng Shao , Sylvain Gigan

Encoding of spectral information onto monochrome imaging cameras is of interest for wavelength multiplexing and hyperspectral imaging applications. Here, the complex spatio-spectral response of a disordered material is used to demonstrate…

Optics · Physics 2017-05-09 Rebecca French , Sylvain Gigan , Otto L. Muskens

We present a widefield fluorescence microscope that integrates an event-based image sensor (EBIS) with a CMOS image sensor (CIS) for ultra-fast microscopy with spectral distinction capabilities. The EBIS achieves temporal resolution of…

Optics · Physics 2024-10-22 Richard G. Baird , Apratim Majumder , Rajesh Menon

We propose a scheme to retrieve the size parameters of a nano-particle on a glass substrate at a scale much smaller than the wavelength. This is achieved by illuminating the particle using two plane waves to create rich and non-trivial…

Optics · Physics 2018-06-27 Zheng Xi , H. P. Urbach

Light-sheet fluorescence microscopy (LSFM) is used to capture volume images of biological specimens. It offers high contrast deep inside densely fluorescence labelled samples, fast acquisition speed and minimal harmful effects on the…

Image and Video Processing · Electrical Eng. & Systems 2024-04-05 Niklas Rottmayer , Claudia Redenbach , Florian Fahrbach

While Fourier ptychography (FP) offers super-resolution for macroscopic imaging, its real-world application is severely hampered by atmospheric turbulence, a challenge largely unaddressed in existing macroscopic FP research operating under…

A rigorous mathematical model and an efficient computational method are proposed to solving the inverse elastic surface scattering problem which arises from the near-field imaging of periodic structures. We demonstrate how an enhanced…

Analysis of PDEs · Mathematics 2017-12-08 Huaian Diao , Peijun Li , Xiaokai Yuan

Speckles arise when coherent light interacts with biological tissues. Information retrieval from speckles is desired yet challenging, requiring understanding or mapping of the multiple scattering process, or reliable capability to reverse…

Image and Video Processing · Electrical Eng. & Systems 2020-05-05 Huanhao Li , Zhipeng Yu , Yunqi Luo , Shengfu Cheng , Lihong V. Wang , Yuanjin Zheng , Puxiang Lai

Manipulating broadband fields in scattering media is a modern challenge across photonics and other wave domains. Recent studies have shown that complex propagation in scattering media can be harnessed to manipulate broadband light wave…

We present an imaging technique that allows the recovery of the transparency profile of wavelength-scale objects with deep subwavelength resolution based on far-field intensity measurements. The approach, interscale mixing microscopy (IMM),…

Optics · Physics 2017-03-21 Sandeep Inampudi , Nicholas Kuhta , Viktor A. Podolskiy

Capturing biological specimens at large scales with sub-micron resolution is crucial for biomedical research, but conventional cameras often can't handle the pixel requirements. While most microscopes use motorized stages to move samples…

Optics · Physics 2025-03-28 Charlie Kersuzan , Aymerick Bazin , Amaury Badon

This paper introduces a new method of data-driven microscope design for virtual fluorescence microscopy. Our results show that by including a model of illumination within the first layers of a deep convolutional neural network, it is…

Image and Video Processing · Electrical Eng. & Systems 2020-04-23 Colin L. Cooke , Fanjie Kong , Amey Chaware , Kevin C. Zhou , Kanghyun Kim , Rong Xu , D. Michael Ando , Samuel J. Yang , Pavan Chandra Konda , Roarke Horstmeyer

Total internal reflection fluorescence (TIRF) microscopy and its variants are key technologies for visualizing the dynamics of single molecules or organelles in live cells. Yet, truely quantitative TIRF remains problematic. One unknown…

Biological Physics · Physics 2017-06-07 Maia Brunstein , Lopamudra Roy , Martin Oheim

One challenge that has long held the attention of scientists is that of clearly seeing objects hidden by turbid media, as smoke, fog or biological tissue, which has major implications in fields such as remote sensing or early diagnosis of…

Optics · Physics 2014-11-12 V. Durán , F. Soldevila , E. Irles , P. Clemente , E. Tajahuerce , P. Andrés , J. Lancis

Super-resolution microscopy is crucial for imaging sub-wavelength biological structures. However, most techniques rely on nonlinear saturation or stochastic switching of emitters, limiting imaging speed and increasing phototoxicity. Here,…

A simple method was developed to observe the interference patterns of the light reflected by the interfaces of thin liquid films. Employing a fluorescent microscope with epi-illumination, we collected the 2D patterns of interference fringes…

Optics · Physics 2010-06-14 V. Berejnov , D. Li

Wavefront shaping makes it possible to form a focus through opaque scattering materials. In some cases, this focus may be scanned over a small distance using the optical memory effect. However, in many cases of interest, the optical memory…

Optics · Physics 2020-01-08 Bahareh Mastiani , Tzu-Lun Ohn , Ivo M. Vellekoop

We demonstrate a high-speed method to image objects through a thin scattering medium and around a corner. The method employs a reference object of known shape to retrieve the speckle-like point spread function of the scatterer. We extract…

Recovering the physical attributes of an object's appearance from its images captured under an unknown illumination is challenging yet essential for photo-realistic rendering. Recent approaches adopt the emerging implicit scene…

Computer Vision and Pattern Recognition · Computer Science 2023-04-05 Youjia Zhang , Teng Xu , Junqing Yu , Yuteng Ye , Junle Wang , Yanqing Jing , Jingyi Yu , Wei Yang