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Using optical speckle scanning microscopy [1], we demonstrate that clear images of multiple cells can be obtained through biological scattering tissue, with subcellular resolution and good image quality, as long as the size of the imaging…

Optics · Physics 2023-07-19 Xin Yang , Ye Pu , Demetri Psaltis

Due to the complex of mixed spectral point spread function within memory effect range, it is unreliable and slow to use speckle correlation technology for non-invasive imaging through scattering medium under broadband illumination. The…

Tapered optical fibers (TFs), with diameters gradually reduced from hundreds of microns to the micron scale, offer key advantages over conventional flat optical fibers (FFs), including uniform illumination, efficient long-range signal…

Optics · Physics 2026-02-03 Mingliang Xu , Fangyuan Li , Yuxin Leng , Ruxin Li , Fei He

Optical stellar interferometers have demonstrated milli-arcsecond resolution with few apertures spaced hundreds of meters apart. To obtain rich direct images, many apertures will be needed, for a better sampling of the incoming wavefront.…

Instrumentation and Methods for Astrophysics · Physics 2014-09-17 Arun Surya , Swapan K. Saha , Antoine Labeyrie

We present an analytical description and an experimental realization of interscale mixing microscopy, a diffraction-based imaging technique that is capable of detecting wavelength/10 objects in far-field measurements with both coherent and…

Super-resolution fluorescence microscopy is an important tool in biomedical research for its ability to discern features smaller than the diffraction limit. However, due to its difficult implementation and high cost, the universal…

Fluorescence microscopy has enabled a dramatic development in modern biology by visualizing biological organisms with micrometer scale resolution. However, due to the diffraction limit, sub-micron/nanometer features are difficult to…

Image and Video Processing · Electrical Eng. & Systems 2021-03-10 Varun Mannam , Yide Zhang , Xiaotong Yuan , Scott Howard

We report a novel lensless on-chip microscopy platform based on near-field blind ptychographic modulation. In this platform, we place a thin diffuser in between the object and the image sensor for light wave modulation. By blindly scanning…

Image and Video Processing · Electrical Eng. & Systems 2020-02-13 Shaowei Jiang , Jiakai Zhu , Pengming Song , Chengfei Guo , Zichao Bian , Ruihai Wang , Yikun Huang , Shiyao Wang , He Zhang , Guoan Zheng

Fluorescence microscopy is a critical tool across various disciplines, from materials science to biomedical research, yet it is limited by the diffraction limit of resolution. Advanced super-resolution techniques such as localization…

Phase-sensitive coherent imaging exploits changes in the phases of backscattered light to observe tiny alterations of scattering structures or variations of the refractive index. But moving scatterers or a fluctuating refractive index…

Fluorescent imaging plays a critical role in a myriad of scientific endeavors, particularly in the biological sciences. Three-dimensional imaging of fluorescent intensity often requires serial data acquisition, that is voxel-by-voxel…

Optics · Physics 2015-10-27 Jeffrey J. Field , David G. Winters , Randy A. Bartels

When light propagates through opaque material, the spatial information it holds becomes scrambled, but not necessarily lost. Two classes of techniques have emerged to recover this information: methods relying on optical memory effects, and…

Molecular fluorescence microscopy is a leading approach to super-resolution and nanoscale imaging in life and material sciences. However, super-resolution fluorescence microscopy is often bottlenecked by system-specific calibrations and…

Spatial Frequency Domain Imaging can map tissue scattering and absorption properties over a wide field of view, making it useful for clinical applications such as wound assessment and surgical guidance. This technique has previously…

Biological Physics · Physics 2021-02-24 Mason T. Chen , Melina Papadakis , Nicholas J. Durr

Optical imaging through complex scattering media is one of the major technical challenges with important applications in many research fields, ranging from biomedical imaging, astronomical telescopy, and spatially multiplex optical…

Formation of a bright-field microscopic image of a transparent phase object is described in terms of elementary geometrical optics. Our approach is based on the premise that image replicates the intensity distribution (real or virtual) at…

Optics · Physics 2018-02-14 A. K. Khitrin , M. A. Model

Luminescence imaging is invaluable for studying biological and material systems, particularly when advanced protocols that exploit temporal dynamics are employed. However, implementing such protocols often requires custom instrumentation,…

Instrumentation and Detectors · Physics 2025-09-16 Ian Coghill , Alienor Lahlou , Andrea Lodetti , Shizue Matsubara , Johann Boucle , Thomas Le Saux , Ludovic Jullien

Interferometric scattering microscopy has been a very promising technology for highly sensitive label-free imaging of a broad spectrum of biological nanoparticles from proteins to viruses in a high-throughput manner. Although it can reveal…

Optics · Physics 2021-08-11 Celalettin Yurdakul , Haonan Zong , Yeran Bai , Ji-Xin Cheng , M. Selim Unlu

Optical scattering presents a major obstacle to high resolution imaging in biological tissue and other turbid media. Conventional photoacoustic imaging can partially overcome this obstacle, enabling imaging of optical absorption in the…

Strong scattering medium brings great difficulties to optical imaging, which is also a problem in medical imaging and many other fields. Optical memory effect makes it possible to image through strong random scattering medium. However, this…

Image and Video Processing · Electrical Eng. & Systems 2020-02-19 Enlai Guo , Shuo Zhu , Yan Sun , Lianfa Bai , Jing Han