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We investigate the translocation of a stiff polymer through a nanopore in a membrane, in the presence of binding particles (chaperones) that bind reversibly to the polymer on both sides of the membrane. A bound chaperone covers one…

统计力学 · 物理学 2016-08-16 Tobias Ambjörnsson , Ralf Metzler

Chaperone-assisted translocation through a nanopore embedded in membrane holds a prominent role in the transport of biopolymers. Inspired by classical Brownian ratchet, we develop a theoretical framework characterizing such translocation…

生物物理 · 物理学 2020-02-17 Xining Xu , Yunxin Zhang

We investigate a model of chaperone-assisted polymer translocation through a nanopore in a membrane. Translocation is driven by irreversible random sequential absorption of chaperone proteins that bind to the polymer on one side of the…

统计力学 · 物理学 2015-05-19 Paul Krapivsky , Kirone Mallick

Using Langevin dynamics simulations, we investigate the dynamics of chaperone-assisted translocation of a flexible polymer through a nanopore. We find that increasing the binding energy $\epsilon$ between the chaperone and the chain and the…

软凝聚态物质 · 物理学 2011-08-26 Wancheng Yu , Kaifu Luo

We investigate the translocation of stiff polymers in the presence of binding particles through a nanopore by two-dimensional Langevin dynamics simulations. We find that the mean translocation time shows a minimum as a function of the…

软凝聚态物质 · 物理学 2015-06-12 Wancheng Yu , Yiding Ma , Kaifu Luo

Polymer translocation through a nanopore in a membrane investigated theoretically. Recent experiments on voltage-driven DNA and RNA translocations through a nanopore indicate that the size and geometry of the pore are important factors in…

统计力学 · 物理学 2009-11-07 Elena Slonkina , Anatoly B. Kolomeisky

The translocation dynamics of a polymer chain through a nanopore in the absence of an external driving force is analyzed by means of scaling arguments, fractional calculus, and computer simulations. The problem at hand is mapped on a one…

软凝聚态物质 · 物理学 2007-07-29 J. L. A. Dubbeldam , A. Milchev , V. G. Rostiashvili , T. A. Vilgis

The transport of polymers with folded configurations across membrane pores is investigated theoretically by analyzing simple discrete stochastic models. The translocation dynamics is viewed as a sequence of two events: motion of the folded…

统计力学 · 物理学 2009-11-13 Stanislav Kotsev , Anatoly B. Kolomeisky

Polymer translocation through a nanometer-scale pore assisted by chaperones binding to the polymer is a process encountered in vivo for proteins. Studying the relevant models by computer simulations is computationally demanding.…

生物物理 · 物理学 2017-12-18 P. M. Suhonen , R. P. Linna

We study the translocation dynamics of a polymer chain threaded through a nanopore by an external force. By means of diverse methods (scaling arguments, fractional calculus and Monte Carlo simulation) we show that the relevant dynamic…

统计力学 · 物理学 2007-07-29 J. L. A. Dubbeldam , A. Milchev , V. G. Rostiashvili , T. A. Vilgis

Chaperones are binding proteins which work as a driving force to bias the biopolymer translocation by binding to it near the pore and preventing its backsliding. Chaperones may have different spatial distribution. Recently we show the…

生物物理 · 物理学 2019-12-03 Rouhollah Haji Abdolvahab

The motion of polymers with inhomogeneous structure through nanopores is discussed theoretically. Specifically, we consider the translocation dynamics of polymers consisting of double-stranded and single-stranded blocks. Since only the…

统计力学 · 物理学 2009-11-11 Stanislav Kotsev , Anatoly B. Kolomeisky

Force-driven translocation of a macromolecule through a nanopore is investigated by taking into account the monomer-pore friction as well as the "crowding" of monomers on the {\it trans} - side of the membrane which counterbalance the…

软凝聚态物质 · 物理学 2014-10-06 Johan L. A. Dubbeldam , V. G. Rostiashvili , T. A. Vilgis

The threading of a polymer chain through a small pore is a classic problem in polymer dynamics and underlies nanopore sensing technology. However important experimental aspects of the polymer motion in a solid-state nanopore, such as an…

生物物理 · 物理学 2016-07-18 Nicholas A. W. Bell , Ulrich F. Keyser

Using Langevin dynamics simulations, we investigate the dynamics of polymer translocation into a circular nanocontainer through a nanopore under a driving force $F$. We observe that the translocation probability initially increases and then…

软凝聚态物质 · 物理学 2012-12-06 Kehong Zhang , Kaifu Luo

Chaperone-assisted biopolymer translocation is the main model proposed for translocation \textit{in vivo}. A dynamical Monte Carlo method is used to simulate the translocation of a stiff homopolymer through a nanopore driven by chaperones.…

生物物理 · 物理学 2019-12-03 Rouhollh Haji Abdolvahab

In this work we study the assisted translocation of a polymer across a membrane nanopore, inside which a molecular motor exerts a force fuelled by the hydrolysis of ATP molecules. In our model the motor switches to its active state for a…

软凝聚态物质 · 物理学 2018-03-26 A. Fiasconaro , J. J. Mazo , F. Falo

We study voltage driven translocation of a single stranded (ss) DNA through a membrane channel. Our model, based on a master equation (ME) approach, investigates the probability density function (pdf) of the translocation times, and shows…

统计力学 · 物理学 2007-05-23 O. Flomenbom , J. Klafter

Polymer translocation is a promising strategy for the next-generation DNA sequencing technologies. The use of biological and synthetic nano-pores, however, still suffers from serious drawbacks. In particular, the width of the membrane layer…

软凝聚态物质 · 物理学 2017-11-30 Timothee Menais , Stefano Mossa , Arnaud Buhot

We suggest a theoretical description of the force-induced translocation dynamics of a polymer chain through a nanopore. Our consideration is based on the tensile (Pincus) blob picture of a pulled chain and the notion of propagating front of…

软凝聚态物质 · 物理学 2012-04-17 J. L. A. Dubbeldam , V. G. Rostiashvili , A. Milchev , T. A. Vilgis
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