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DNA/RNA synthesis precursors are especially vulnerable to damage induced by reactive oxygen species occurring following oxidative stress. Guanosine triphosphates are the prevalent oxidized nucleosites, which can be misincorporated during…

Tuberculosis relapse is a barrier to shorter treatment. It is thought that lipid rich cells, phenotypically resistant to antibiotics, may play a major role. Most studies investigating relapse use sputum samples although tissue bacteria may…

Since lattice strain and charge density affect various material properties of graphene, a reliable and efficient method is required for quantification of the two variables. While Raman spectroscopy is sensitive and non-destructive, its…

介观与纳米尺度物理 · 物理学 2020-08-10 Gwanghyen Ahn , Sunmin Ryu

Confocal Raman microscopy, a highly specific and label-free technique for the microscale study of thick samples, often presents difficulties due to weak Raman signals. Inhomogeneous samples introduce wavefront aberrations that further…

Lipid droplets (LDs) are subcellular organelles with important roles in lipid storage and metabolism and involved in various diseases including cancer, obesity, and diabetes. Conventional methods, however, have limited ability to provide…

生物物理 · 物理学 2016-11-08 Kyoohyun Kim , SeoEun Lee , Jonghee Yoon , JiHan Heo , Chulhee Choi , YongKeun Park

LiDRoSIS is an automated MATLAB-Python software suite for the segmentation and quantification of lipid droplets (LDs) and reactive oxygen species (ROS) in fluorescence microscopy images of irradiated A549 and MCF7 cells exposed to…

医学物理 · 物理学 2026-01-22 Marco Ferreira , Ana Belchior , Teresa Pinheiro , Gil Alves , Maria Lopes

A theoretical model was developed to investigate the rod phototransduction process in the mouse. In particular, we explored the biochemical reactions of several chemical components that contribute to the signaling process into/around the…

生物物理 · 物理学 2015-12-04 Rei Takamoto , Hiraku Nishimori , Akinori Awazu

Over recent decades, lipid membranes have become standard models for examining the biophysics and biochemistry of cell membranes. Interrogation of lipid domains within biomembranes is generally done with fluorescence microscopy via…

生物物理 · 物理学 2018-03-30 Stephen H. Donaldson , Hilton B. de Aguiar

Resistive Pulse Sensing (RPS) is a key label-free technology to measure particles and single-cell size distribution. As a growing corpus of evidence supports that cancer cells exhibit distinct mechanical phenotypes from healthy cells,…

生物物理 · 物理学 2020-04-01 Antoine Riaud , Anh L. P. Thai , Wei Wang , Valerie Taly

The local thermal conductivity (\k{appa}) is a pivotal biophysical parameter, governing intracellular heat flux and underlying functional processes like metabolic regulation and stress response. However, label-free mapping with sub-micron…

生物物理 · 物理学 2026-03-04 Jiahua Zhang , Yong Hou , Xinhao Hu , Yicheng Wang , Madoka Suzuki , Bo Gao , Zhiqin Chu

Receptor-induced apoptosis is a complex signal transduction pathway involving numerous protein/protein interactions and post-transcriptional modifications. The response to death receptor stimulation varies significantly from one cell line…

定量方法 · 定量生物学 2017-12-20 François Bertaux , Dirk Drasdo , Gregory Batt

Despite fluorescent cell-labelling being widely employed in biomedical studies, some of its drawbacks are inevitable, with unsuitable fluorescent probes or probes inducing a functional change being the main limitations. Consequently, the…

Raman spectroscopy is one of the widely used methods in the analysis of various samples including carbon-based materials. This study aimed to identify the number of layers and defects in graphene using micro-Raman spectroscopy. More…

光学 · 物理学 2024-03-21 Somayeh Gholipour , Maryam Bahreini , Mohamad Reza Jafarfard

Coherent anti-Stokes Raman scattering (CARS) microscopy offers label-free chemical imaging capabilities, but its performance is constrained by small Raman scattering cross-section, strong non-resonant background (NRB), and limited…

Hydrogen detection is of great importance in chemical and energy industries. Optical fiber hydrogen sensors show flexibility and compactness and have the potential for distributed analysis. However, traditional fiber sensors encounter a…

光学 · 物理学 2019-05-16 F. Yang , Y. Zhao , Y. Qi , Y. Z. Tan , H. L. Ho , W. Jin

In life sciences, fluorescent labeling techniques are used to study molecular structures and interactions of cells. However, this type of cell imaging has its own limitations, one of which is that the process of staining the cells could be…

图像与视频处理 · 电气工程与系统科学 2020-05-07 Eunjae Kim

Random lateral translocation of lipids and proteins is a universal process on membrane surfaces. Local aggregation or organization of lipids and proteins can be induced when this lateral random diffusion is mediated by the electrostatic…

定量方法 · 定量生物学 2015-06-03 Y. C. Zhou

Cell tracking is a subject of active research gathering great interest in medicine and biology. Positron emission tomography (PET) is well suited for tracking radio-labeled cells in vivo due to its exceptional sensitivity and whole-body…

医学物理 · 物理学 2025-09-26 Nils Marquardt , Tobias Hengsbach , Marco Mauritz , Benedikt Wirth , Klaus Schäfers

We formulate the continuum field equations and constitutive equations that govern deformation, stress, and electric current flow in a Li-ion half-cell. The model considers mass transport through the system, deformation and stress in the…

计算物理 · 物理学 2011-08-01 Allan F. Bower , Pradeep R. Guduru , Vijay A. Sethuraman

Understanding how glioblastoma (GBM) emerges from initially healthy glial tissue requires models that integrate bioelectrical, metabolic, and multicellular dynamics. This work introduces an ASAL-inspired agent-based framework that simulates…

生物物理 · 物理学 2025-11-26 Wiktoria Agata Pawlak