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Among super-resolution microscopy techniques, structured illumination microscopy (SIM) shows great advances of low phototoxicity, high speed, and excellent performance in long-term dynamic observation, making it especially suitable for live…

光学 · 物理学 2025-02-07 Han Wang , Wenshu Wang , Xinzhu Xu , Meiqi Li , Peng Xi

Structured illumination microscopy (SIM) is one of the most versatile super-resolution techniques. Yet, its application to live imaging has been so far mainly limited to fluorescent and stationary specimens. Here, we present advancements in…

Structured illumination microscopy (SIM) has emerged as an essential technique for 3D and live-cell super-resolution imaging. However, to date, there has not been a dedicated workshop or journal issue covering the various aspects of SIM,…

Structured illumination microscopy (SIM) has become an important technique for optical super-resolution imaging because it allows a doubling of image resolution at speeds compatible for live-cell imaging. However, the reconstruction of SIM…

图像与视频处理 · 电气工程与系统科学 2020-03-26 Charles N. Christensen , Edward N. Ward , Pietro Lio , Clemens F. Kaminski

Structured illumination microscopy (SIM) is an important super-resolution based microscopy technique that breaks the diffraction limit and enhances optical microscopy systems. With the development of biology and medical engineering, there…

图像与视频处理 · 电气工程与系统科学 2021-11-18 Xi Cheng , Jun Li , Qiang Dai , Zhenyong Fu , Jian Yang

Structured illumination microscopy (SIM) is an optical super-resolution technique that enables live-cell imaging beyond the diffraction limit. Reconstruction of SIM data is prone to artefacts, which becomes problematic when imaging highly…

图像与视频处理 · 电气工程与系统科学 2022-03-02 Charles N. Christensen , Meng Lu , Edward N. Ward , Pietro Lio , Clemens F. Kaminski

Structured illumination microscopy (SIM) is a very important super-resolution microscopy technique, which provides high speed super-resolution with about two-fold spatial resolution enhancement. Several attempts aimed at improving the…

计算机视觉与模式识别 · 计算机科学 2016-02-23 Amit Lal , Chunyan Shan , Peng Xi

By exploiting the nonlinear responses of the fluorescent probes, the spatial resolution of structured illumination microscopy(SIM) can be further increased. However, due to the complex reconstruction process, the traditional reconstruction…

Structured Illumination Microscopy (SIM) allows access to spatial information beyond the diffraction limit by folding high frequency components into the optical system's base-band. Using various algorithmic techniques, an image containing…

光学 · 物理学 2024-10-16 Doron Shterman , Guy Bartal

Structured illumination microscopy (SIM) enables live cell, super-resolution imaging at high speeds. SIM uses sophisticated optical systems to generate pre-determined excitation light patterns, and reconstruction algorithms to enhance the…

Structured illumination microscopy (SIM) provides images of fluorescent objects at an enhanced resolution greater than that of conventional epifluorescence wide-field microscopy. Initially demonstrated in 1999 to enhance the lateral…

光学 · 物理学 2022-03-09 James D. Manton

Super-resolution Structured Illumination Microscopy (SR-SIM) enables fluorescence microscopy beyond the diffraction limit at high frame rates. Compared to other super-resolution microscopy techniques, the low photon fluence used in SR-SIM…

Structured illumination microscopy (SIM) has emerged as a widely adopted super-resolution fluorescence imaging modality, offering high speed, low phototoxicity, large field-of-view, and compatibility with conventional probes. However, when…

图像与视频处理 · 电气工程与系统科学 2026-03-19 Jiaming Qian , Jing Feng , Hongjun Wu , Maoxian Zhang , Dongqin Lu , Tianchi Kang , Xinyu Han , Qian Chen , Chao Zuo

Structured illumination microscopy (SIM) is a pivotal technique for dynamic subcellular imaging in live cells. Conventional SIM reconstruction algorithms depend on accurately estimating the illumination pattern and can introduce artefacts…

光学 · 物理学 2025-03-17 Long-Kun Shan , Ze-Hao Wang , Tong-Tian Weng , Xiang-Dong Chen , Fang-Wen Sun

Structured Illumination Microscopy (SIM) is an imaging technique for achieving both super-resolution (SR) and optical sectioning (OS) in wide-field microscopy. It consists in illuminating the sample with periodic patterns at different…

In this communication, a fast reconstruction algorithm is proposed for fluorescence \textit{blind} structured illumination microscopy (SIM) under the sample positivity constraint. This new algorithm is by far simpler and faster than…

数据分析、统计与概率 · 物理学 2016-11-18 S. Labouesse , M. Allain , J. Idier , S. Bourguignon , A. Negash , P. Liu , A. Sentenac

Super-resolution structured illumination microscopy (SR-SIM) is a widely used technique for enhancing the resolution of fluorescence imaging beyond the diffraction limit. Most existing SR-SIM methods rely on Moir\'e effect-based physical…

光学 · 物理学 2025-02-27 Junzheng Peng , Jiahao Xian , Xi Lin , Manhong Yao , Shiping Li , Jingang Zhong

High-content biological microscopy targets high-resolution imaging across large fields-of-view (FOVs). Recent works have demonstrated that computational imaging can provide efficient solutions for high-content microscopy. Here, we use…

图像与视频处理 · 电气工程与系统科学 2019-03-28 Li-Hao Yeh , Shwetadwip Chowdhury , Laura Waller

Structured illumination microscopy (SIM) improves resolution by down-modulating high-frequency information of an object to fit within the passband of the optical system. Generally, the reconstruction process requires prior knowledge of the…

计算机视觉与模式识别 · 计算机科学 2017-01-13 Li-Hao Yeh , Lei Tian , Laura Waller

The blind structured illumination microscopy (SIM) strategy proposed in (Mudry et al., 1992) is fully re-founded in this paper, unveiling the central role of the sparsity of the illumination patterns in the mechanism that drives…

数据分析、统计与概率 · 物理学 2017-04-26 Simon Labouesse , Awoke Negash , Jérôme Idier , Sébastien Bourguignon , Thomas Mangeat , Penghuan Liu , Anne Sentenac , Marc Allain
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