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相关论文: Effective stripe artefact removal by a variational…

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Light-sheet fluorescence microscopy (LSFM) is a cutting-edge volumetric imaging technique that allows for three-dimensional imaging of mesoscopic samples with decoupled illumination and detection paths. Although the selective excitation…

图像与视频处理 · 电气工程与系统科学 2022-06-28 Yu Liu , Kurt Weiss , Nassir Navab , Carsten Marr , Jan Huisken , Tingying Peng

We report two techniques to mitigate stripe artifacts in light-sheet fluorescence imaging. The first uses an image processing algorithm called the multidirectional stripe remover (MDSR) method to filter stripes from an existing image. The…

仪器与探测器 · 物理学 2023-11-03 Seyyed Muhammad Salili , Matt Harrington , Douglas J. Durian

Light sheet fluorescence microscopy (LSFM) has transformed the way we visualize biological tissues in three dimensions, offering high-resolution imaging while minimizing photo-induced damage to the samples. Recent breakthroughs in…

光学 · 物理学 2025-05-27 Alireza Tabatabaei Mashayekh , Jeremy Witzens

We report on the recently emerging (Laser) Light Sheet based Fluorescence Microscopy field (LSFM). The techniques used in this field allow to study and visualize biomedical objects non-destructively in high-resolution through virtual…

仪器与探测器 · 物理学 2011-06-17 Jan A. N. Buytaert , Emilie Descamps , Dominique Adriaens , Joris J. J. Dirckx

We developed a Bessel light sheet fluorescence microscopy (LSFM) system to enable high-speed, wide-field intra-vital imaging of zebrafish and other thick biological samples. This system uses air objectives for the convenient mounting of…

光学 · 物理学 2023-11-07 Chuhui Wang , Jiaju Chen , Cuiyi Peng , Zhenglin Chen , Dongmei Yu , Peiwu Qin

In recent years, light-sheet fluorescence microscopy (LSFM) has emerged as a powerful tool for visualizing and analyzing cancer tissue samples, including patient-derived specimens, organoids, biopsies, and murine models. In this work, we…

A lensfree spectral light-field fusion microscopy (LSLFM) system is presented for enabling contrast- and resolution-enhanced imaging of biological specimens. LSLFM consists of a pulsed multispectral lensfree microscope for capturing…

Light sheet fluorescence microscopy is able to image large specimen with high resolution by imaging the sam- ples from multiple angles. Multi-view deconvolution can significantly improve the resolution and contrast of the images, but its…

Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique in biomedical research that uses the fluorophore decay rate to provide additional contrast in fluorescence microscopy. However, at present, the calculation, analysis,…

图像与视频处理 · 电气工程与系统科学 2021-06-09 Varun Mannam , Yide Zhang , Xiaotong Yuan , Cara Ravasio , Scott S. Howard

High-speed image acquisition in light microscopy is essential for a wide range of applications, including observing dynamic biological processes and enabling high-throughput sample analysis. However, traditional imaging speeds are often…

It was recently demonstrated [J. Electron. Imaging, 25(2), 2016] that one can perform fast non-local means (NLM) denoising of one-dimensional signals using a method called lifting. The cost of lifting is independent of the patch length,…

计算机视觉与模式识别 · 计算机科学 2018-03-14 Sanjay Ghosh , Kunal N. Chaudhury

Differential Dynamic Microscopy (DDM) analyzes traditional real-space microscope images to extract information on sample dynamics in a way akin to light scattering, by decomposing each image in a sequence into Fourier modes, and evaluating…

软凝聚态物质 · 物理学 2017-11-10 Fabio Giavazzi , Paolo Edera , Peter J. Lu , Roberto Cerbino

Focused ion beam (FIB) tomography provides high resolution volumetric images on a micro scale. However, due to the physical acquisition process the resulting images are often corrupted by a so-called curtaining or waterfall effect. In this…

数值分析 · 数学 2016-04-06 Jan Henrik Fitschen , Jianwei Ma , Sebastian Schuff

Destriping is a classical problem in remote sensing image processing. Although considerable effort has been made to remove stripes, few of the existing methods can eliminate stripe noise with arbitrary orientations. This situation makes the…

计算机视觉与模式识别 · 计算机科学 2018-09-07 Xinxin Liu , Xiliang Lu , Huanfeng Shen , Qiangqiang Yuan , Liangpei Zhang

Atomic Force Microscopy (AFM) enables high-resolution surface imaging at the nanoscale, yet the output is often degraded by artifacts introduced by environmental noise, scanning imperfections, and tip-sample interactions. To address this…

计算机视觉与模式识别 · 计算机科学 2026-02-05 Juntao Zhang , Angona Biswas , Jaydeep Rade , Charchit Shukla , Juan Ren , Anwesha Sarkar , Adarsh Krishnamurthy , Aditya Balu

X-ray computed tomography (CT) is widely utilized in the medical, industrial, and other fields to nondestructively generate three-dimensional structural images of objects. However, CT images are often affected by various artifacts, with…

医学物理 · 物理学 2025-05-27 Yang Zou , Meili Qi , Jianhua Zhang , Difei Zhang , Shuwei Wang , Jiale Zhang , Shengkun Yao , Huaidong Jiang

Fluorescence microscopy is a powerful tool for imaging biological samples with molecular specificity. In contrast, phase microscopy provides label-free measurement of the sample's refractive index (RI), which is an intrinsic optical…

光学 · 物理学 2022-01-26 Yi Xue , David Ren , Laura Waller

Automatic plant recognition and disease analysis may be streamlined by an image of a complete, isolated leaf as an initial input. Segmenting leaves from natural images is a hard problem. Cluttered and complex backgrounds: often composed of…

计算机视觉与模式识别 · 计算机科学 2017-09-20 N. Anantrasirichai , Sion Hannuna , Nishan Canagarajah

Fluorescent imaging plays a critical role in a myriad of scientific endeavors, particularly in the biological sciences. Three-dimensional imaging of fluorescent intensity often requires serial data acquisition, that is voxel-by-voxel…

光学 · 物理学 2015-10-27 Jeffrey J. Field , David G. Winters , Randy A. Bartels

Immunofluorescent (IF) imaging is crucial for visualizing biomarker expressions, cell morphology and assessing the effects of drug treatments on sub-cellular components. IF imaging needs extra staining process and often requiring cell…

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