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We introduce MINFLUX, a concept for localizing photon emitters in space. By probing the emitter with a local intensity minimum of excitation light, MINFLUX minimizes the fluorescence photons needed for high localization precision. A 22-fold…

Dark-field X-ray imaging visualizes structural inhomogeneities through small-angle scattering, but existing directional methods are confined to the micrometer scale. While recent advances have extended dark-field capabilities to nanoscale…

The diffraction of light imposes a fundamental limit on the resolution of light microscopes. This limit can be circumvented by creating and exploiting independent behaviors of the sample at length scales below the diffraction limit. In…

生物物理 · 物理学 2024-06-19 Mohamadreza Fazel , Michael J. Wester

The detection of individual photons is an inherently binary mechanism, revealing either their absence or presence while concealing their spectral information. For multi-color imaging techniques, such as single photon spectroscopy,…

仪器与探测器 · 物理学 2016-09-27 O. Kahl , S. Ferrari , V. Kovalyuk , A. Vetter , G. Lewes-Malandrakis , C. Nebel , A. Korneev , G. Goltsman , W. Pernice

Purpose: Handheld gamma cameras with coded aperture collimators are under investigation for intraoperative imaging in nuclear medicine. Coded apertures are a promising collimation technique for applications such as lymph node localization…

医学物理 · 物理学 2024-01-22 Tobias Meißner , Laura Antonia Cerbone , Paolo Russo , Werner Nahm , Jürgen Hesser

Molecular fluorescence microscopy is a leading approach to super-resolution and nanoscale imaging in life and material sciences. However, super-resolution fluorescence microscopy is often bottlenecked by system-specific calibrations and…

Fluorescence microscopy is a critical tool across various disciplines, from materials science to biomedical research, yet it is limited by the diffraction limit of resolution. Advanced super-resolution techniques such as localization…

We propose a straightforward sample-based technique to calibrate the axial detection in 3D single molecule localization microscopy (SMLM). Using microspheres coated with fluorescent molecules, the calibration curves of PSF-shaping- or…

Although several optical techniques have been recently developed in order to overcome the resolution limit in microscopy, the imaging of sub-wavelength features is still a real challenge. In practise, super-resolution techniques remain…

物理教育 · 物理学 2019-07-24 Stephane Perrin , Keshia Badu , Paul Montgomery , Sylvain Lecler

The photo-kinetics of fluorescent molecules have enabled the circumvention of far-field optical diffraction-limit. Despite its enormous potential, the necessity to label the sample may adversely influence the delicate biology under…

A novel finite-element method for calculating the illumination-dependence of absorption in three-dimensional nanostructures is presented based on the RF module of the COMSOL software package. This method is capable of numerically…

光学 · 物理学 2015-05-28 Mária Csete , Áron Sipos , Faraz Najafi , Xiaolong Hu , Karl K. Berggren

Here we propose a new design paradigm for a superconducting nanowire single photon detector that uses a multi-layer architecture that places the electric leads beneath the nanowires. This allows for a very large number of detector elements,…

光学 · 物理学 2015-06-04 A. Matthew Smith

Structured illumination microscopy (SIM) achieves superresolution in fluorescence imaging through patterned illumination and computational image reconstruction, yet current methods require bulky, costly modulation optics and high-precision…

光学 · 物理学 2024-04-15 Tamal Roy , Peter T. Brown , Douglas P. Shepherd , Lisa V. Poulikakos

Single-beam super-resolution microscopy, also known as superlinear microscopy, exploits the nonlinear response of fluorescent probes in confocal microscopy. The technique requires no complex purpose-built system, light field modulation, or…

Strong enhancement of molecular circular dichroism has the potential to enable efficient asymmetric photolysis, a method of chiral separation that has conventionally been impeded by insufficient yield and low enantiomeric excess. Here, we…

Scanning X-ray nanodiffraction microscopy is a powerful technique for spatially resolving nanoscale structural morphologies by diffraction contrast. One of the critical challenges in experimental nanodiffraction data analysis is posed by…

应用物理 · 物理学 2024-06-26 Aileen Luo , Tao Zhou , Martin V. Holt , Andrej Singer , Mathew J. Cherukara

We propose a novel x-ray imaging system, Multi-Image X-ray Interferometer Module (MIXIM), with which a very high angular resolution can be achieved even with a small system size. MIXIM is composed of equally-spaced multiple slits and an…

Controlling the propagation and coupling of light to sub-wavelength antennas is a crucial prerequisite for many nanoscale optical devices. Recently, the main focus of attention has been directed towards high-refractive-index materials such…

光学 · 物理学 2016-05-04 Martin Neugebauer , Paweł Woźniak , Ankan Bag , Gerd Leuchs , Peter Banzer

Confocal microscopy has long been a cornerstone technique for visualizing complex interactions and processes within cellular structures. However, achieving super-resolution imaging of multiple organelles and their interactions…

光学 · 物理学 2025-08-19 Qinglin Chen , Luwei Wang , Jia Li , Dan Shao , Xiaoyu Weng , Liwei Liu , Dayong Jin , Junle Qu

The requirement of a large number of electronic channels poses a big challenge for Micro-pattern Gas Detector (MPGD) to achieve good spatial resolution. By using the redundancy that at least two neighboring strips record the signal of a…

仪器与探测器 · 物理学 2016-08-03 Bin-Xiang Qi , Shu-Bin Liu , Heng Ji , Zhong-Tao Shen , Si-Yuan Ma , Hong-Bang Liu , Wen-Qian Huang , Qi An