Aberration-free calibration for 3D single molecule localization microscopy
Optics
2018-02-14 v1 Biological Physics
Abstract
We propose a straightforward sample-based technique to calibrate the axial detection in 3D single molecule localization microscopy (SMLM). Using microspheres coated with fluorescent molecules, the calibration curves of PSF-shaping- or intensity-based measurements can be obtained for any required depth range from a few hundreds of nanometers to several tens of microns. This experimental method takes into account the effect of the spherical aberration without requiring computational correction.
Cite
@article{arxiv.1710.04957,
title = {Aberration-free calibration for 3D single molecule localization microscopy},
author = {Clément Cabriel and Nicolas Bourg and Guillaume Dupuis and Sandrine Lévêque-Fort},
journal= {arXiv preprint arXiv:1710.04957},
year = {2018}
}
Comments
8 pages, 2 figures. Submitted to Optics Letters on October 12th, 2017