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A major challenge in single-particle cryo-electron microscopy (cryo-EM) is that the orientations adopted by the 3D particles prior to imaging are unknown; yet, this knowledge is essential for high-resolution reconstruction. We present a…

机器学习 · 计算机科学 2021-04-14 Jelena Banjac , Laurène Donati , Michaël Defferrard

Cryo-electron microscopy (cryo-EM) is capable of producing reconstructed 3D images of biomolecules at near-atomic resolution. As such, it represents one of the most promising imaging techniques in structural biology. However, raw cryo-EM…

定量方法 · 定量生物学 2021-05-25 Nina Miolane , Frédéric Poitevin , Yee-Ting Li , Susan Holmes

Cryo-Electron Microscopy (Cryo-EM) is a Nobel prize-winning technology for determining the 3D structure of particles at near-atomic resolution. A fundamental step in the recovering of the 3D single-particle structure is to align its 2D…

图像与视频处理 · 电气工程与系统科学 2021-01-12 Koby Bibas , Gili Weiss-Dicker , Dana Cohen , Noa Cahan , Hayit Greenspan

Cryo-electron microscopy (cryo-EM) has become a tool of fundamental importance in structural biology, helping us understand the basic building blocks of life. The algorithmic challenge of cryo-EM is to jointly estimate the unknown 3D poses…

计算机视觉与模式识别 · 计算机科学 2022-09-01 Axel Levy , Frédéric Poitevin , Julien Martel , Youssef Nashed , Ariana Peck , Nina Miolane , Daniel Ratner , Mike Dunne , Gordon Wetzstein

Cryo-electron microscopy (cryo-EM) allows for the high-resolution reconstruction of 3D structures of proteins and other biomolecules. Successful reconstruction of both shape and movement greatly helps understand the fundamental processes of…

计算机视觉与模式识别 · 计算机科学 2023-10-12 Xinhang Liu , Yan Zeng , Yifan Qin , Hao Li , Jiakai Zhang , Lan Xu , Jingyi Yu

Determining the three-dimensional structure of proteins and protein complexes at atomic resolution is a fundamental task in structural biology. Over the last decade, remarkable progress has been made using "single particle" cryo-electron…

数值分析 · 数学 2017-06-05 Alex Barnett , Leslie Greengard , Andras Pataki , Marina Spivak

Accurate pose estimation and shift correction are key challenges in cryo-EM due to the very low SNR, which directly impacts the fidelity of 3D reconstructions. We present an approach for pose estimation in cryo-EM that leverages…

计算机视觉与模式识别 · 计算机科学 2025-07-22 Kaishva Chintan Shah , Virajith Boddapati , Karthik S. Gurumoorthy , Sandip Kaledhonkar , Ajit Rajwade

Cryo-electron microscopy (cryo-EM) is a powerful technique for determining the structure of proteins and other macromolecular complexes at near-atomic resolution. In single particle cryo-EM, the central problem is to reconstruct the…

定量方法 · 定量生物学 2020-02-18 Ellen D. Zhong , Tristan Bepler , Joseph H. Davis , Bonnie Berger

We propose a framework to jointly determine the deformation parameters and reconstruct the unknown volume in electron cryotomography (CryoET). CryoET aims to reconstruct three-dimensional biological samples from two-dimensional projections.…

图像与视频处理 · 电气工程与系统科学 2023-10-17 Valentin Debarnot , Sidharth Gupta , Konik Kothari , Ivan Dokmanic

Single-particle cryo-electron microscopy (cryo-EM) reconstructs the three-dimensional (3D) structure of bio-molecules from a large set of 2D projection images with random and unknown orientations. A crucial step in the single-particle…

图像与视频处理 · 电气工程与系统科学 2020-06-24 Mona Zehni , Laurène Donati , Emmanuel Soubies , Zhizhen J. Zhao , Michael Unser

Determining the 3D structures of biological molecules is a key problem for both biology and medicine. Electron Cryomicroscopy (Cryo-EM) is a promising technique for structure estimation which relies heavily on computational methods to…

机器学习 · 统计学 2015-01-29 Ali Punjani , Marcus A. Brubaker

Cryogenic electron microscopy (cryo-EM) is an invaluable technique for determining high-resolution three-dimensional structures of biological macromolecules using transmission particle images. The inherent symmetry in these macromolecules…

最优化与控制 · 数学 2024-11-06 Qi Zhang , Chenglong Bao , Hai Lin , Mingxu Hu

Pose estimation from unordered images is fundamental for 3D reconstruction, robotics, and scientific imaging. Recent geometric foundation models, such as DUSt3R, enable end-to-end dense 3D reconstruction but remain underexplored in…

计算机视觉与模式识别 · 计算机科学 2025-10-15 Jiakai Zhang , Shouchen Zhou , Haizhao Dai , Xinhang Liu , Peihao Wang , Zhiwen Fan , Yuan Pei , Jingyi Yu

Single-particle electron cryomicroscopy (cryo-EM) is an increasingly popular technique for elucidating the three-dimensional structure of proteins and other biologically significant complexes at near-atomic resolution. It is an imaging…

计算物理 · 物理学 2020-04-01 Amit Singer , Fred J. Sigworth

Cryo-electron microscopy (cryo-EM) is a powerful imaging technique for reconstructing three-dimensional molecular structures from noisy tomographic projection images of randomly oriented particles. We introduce a new data fusion framework,…

计算机视觉与模式识别 · 计算机科学 2025-12-15 Joe Kileel , Oscar Mickelin , Amit Singer , Sheng Xu

A major challenge in single particle reconstruction from cryo-electron microscopy is to establish a reliable ab-initio three-dimensional model using two-dimensional projection images with unknown orientations. Common-lines based methods…

机器学习 · 计算机科学 2013-04-11 Lanhui Wang , Amit Singer , Zaiwen Wen

Cryo-electron microscopy (cryo-EM) has achieved near-atomic level resolution of biomolecules by reconstructing 2D micrographs. However, the resolution and accuracy of the reconstructed particles are significantly reduced due to the…

计算机视觉与模式识别 · 计算机科学 2024-01-03 Jing Zhang , Tengfei Zhao , ShiYu Hu , Xin Zhao

Cryo-Electron Microscopy (cryo-EM) has emerged as a key technology to determine the structure of proteins, particularly large protein complexes and assemblies in recent years. A key challenge in cryo-EM data analysis is to automatically…

生物大分子 · 定量生物学 2023-04-04 Nabin Giri , Raj S. Roy , Jianlin Cheng

Cryo-electron microscopy (EM) single particle reconstruction is an entirely general technique for 3D structure determination of macromolecular complexes. However, because the images are taken at low electron dose, it is extremely hard to…

计算机视觉与模式识别 · 计算机科学 2019-04-17 Yifeng Fan , Zhizhen Zhao

A major challenge in single particle reconstruction methods using cryo-electron microscopy is to attain a resolution sufficient to interpret fine details in three-dimensional (3D) macromolecular structures. Obtaining high resolution 3D…

数值分析 · 数学 2013-07-23 Lanhui Wang , Yoel Shkolnisky , Amit Singer
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