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Remote focusing (RF) is a technique that greatly extends the aberration-free axial scan range of an optical microscope. To maximise the diffraction limited depth range in an RF system, the magnification of the relay lenses should be such…

光学 · 物理学 2021-01-05 Sharika Mohanan , Alexander D. Corbett

Remote focusing is widely used in 3D two-photon microscopy and 3D photostimulation because it enables fast axial scanning without moving the objective lens or specimen. However, due to the design constraints of microscope optics, remote…

Oblique plane microscopy is a method enabling light-sheet fluorescence imaging through a single microscope objective lens by focusing on a tilted plane within the sample. To focus the fluorescence emitted by the oblique plane on a camera,…

Multichannel, infinite-conjugate optical systems easily allow implementation of multiple image paths and imaging modes into a single microscope. Traditional optical alignment methods which rely on additional hardware are not always simple…

光学 · 物理学 2024-07-16 Gemma S. Cairns , Brian R. Patton

Imaging specimens over large scales and with a sub-micron resolution is instrumental to biomedical research. Yet, the number of pixels to form such an image usually exceeds the number of pixels provided by conventional cameras. While most…

仪器与探测器 · 物理学 2019-12-03 Gaelle Recher , Pierre Nassoy , Amaury Badon

It has previously been demonstrated in both simulation and experiment that well aligned remote focusing microscopes exhibit residual spherical aberration outside the focal plane. In this work, compensation of the residual spherical…

光学 · 物理学 2023-05-17 Sharika Mohanan , Alexander D. Corbett

The axis tilt of light beam in optical system would introduce the dispersion of orbital angular momentum (OAM) spectrum. To deal with it, a two-step method is proposed and demonstrated. First, the tilt angle of optical axis is identified…

光学 · 物理学 2020-04-27 Peng Zhao , Shikang Li , Yu Wang , Xue Feng , Kaiyu Cui , Fang Liu , Wei Zhang , Yidong Huang

Off-axis parabolic mirrors (OAPs) are occasionally desirable for specialized applications, but are known to introduce field-dependent astigmatic aberrations. In an experiment where optical tweezers are formed by OAPs, another OAP is added…

光学 · 物理学 2025-11-20 Meimei Liu , Yuqi Zhu , Giorgio Gratta

In the rapidly evolving field of optical engineering, precise alignment of multi-lens imaging systems is critical yet challenging, as even minor misalignments can significantly degrade performance. Traditional alignment methods rely on…

光学 · 物理学 2025-07-01 Tomer Slor , Dean Oren , Shira Baneth , Tom Coen , Haim Suchowski

Selective plane illumination microscopy (SPIM) is an optical sectioning imaging approach based on orthogonal light pathways for excitation and detection. The excitation pathway has an inverse relation between the optical sectioning strength…

光学 · 物理学 2024-07-29 Steven J. Sheppard , Peter T. Brown , Douglas P. Shepherd

Current optoacoustic microscopy configurations often have narrow focal ranges that limit their use for fast volumetric imaging applications. Here, the focal range of optoacoustic microscopes is extended by matching the elongated optical…

光学 · 物理学 2021-04-15 Zakiullah Ali , Christian Zakian , Vasilis Ntziachristos

Remote focusing of light in a graded-index medium via mode interference is demonstrated using exact analytical solutions of the wave equation. Strong focusing of light occurs at extremely long distances and it revivals periodically with…

光学 · 物理学 2015-12-09 Nikolai I. Petrov

In this work, we measure and model tilted x-ray refractive lenses to investigate their effects on an x-ray beam. The modelling is benchmarked against at-wavelength metrology obtained with x-ray speckle vector tracking experiments (XSVT) at…

Accurate distance measurement in 3D confocal microscopy is important for quantitative analysis, volume visualization and image restoration. However, axial distances can be distorted by both the point spread function and by a…

软凝聚态物质 · 物理学 2014-04-16 Thijs Herman Besseling , Jissy Jose , Alfons van Blaaderen

TianQin is a planned Chinese space-based gravitational wave (GW) observatory with a frequency band of 10-4 to 1Hz. Optical telescopes are essential for the delivery of the measurement beam to support a precise distance measurement between…

天体物理仪器与方法 · 物理学 2023-03-07 Zichao Fan , Zhengbo Zhu , Huiru Ji , Yan Mo , Hao Tan , Lujia Zhao , Shengyi Cao , Donglin Ma

Modern extreme adaptive optics (AO) systems achieving diffraction-limited performance open up new possibilities for instrumentation. Especially important for the fields of spectroscopy and interferometry is that it enables the prospect to…

Angular differential imaging (ADI) (Marois et al. 2006) is an observational technique in high contrast imaging where the telescope is used in pupil tracking mode so that the image of the sky rotates with respect to the optical surfaces.…

地球与行星天体物理 · 物理学 2017-11-28 Michael Bottom , Garreth Ruane , Dimitri Mawet

The far-field resolution of optical imaging systems is restricted by the Abbe diffraction limit, a direct result of the wave nature of light. One successful technological approach to circumventing this limit is to reduce the effective size…

Adaptive optics (AO) have been used to correct wavefronts to achieve diffraction limited point spread functions in a broad range of optical applications, prominently ground-based astronomical telescopes operating in near infra-red. While…

天体物理仪器与方法 · 物理学 2018-10-03 Alexandre J. T. S. Mello , Antonin H. Bouchez , Andrew Szentgyorgyi , Marcos A. van Dam , Henrique Lupinari

Rather than using an adaptive optics (AO) system to correct a telescope s entire pupil, it can instead be used to more finely correct a smaller sub-aperture. Indeed, existing AO systems can be used to correct a sub-aperture 1/3 to 1/2 the…

天体物理学 · 物理学 2009-11-13 E. Serabyn , K. Wallace , M. Troy , B. Mennesson , P. Haguenauer , R. Gappinger , R. Burruss
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