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A closer look at nature has recently brought more interest in exploring and utilizing intra-body communication networks composed of cells as intrinsic, perfectly biocompatible infrastructures to deliver therapeutics. Naturally occurring…

信号处理 · 电气工程与系统科学 2022-07-06 Hamid Khoshfekr Rudsari , Mohammad Zoofaghari , Mladen Veletic , Jacob Bergsland , Ilangko Balasingham

Electron transfer (ET) through proteins, a fundamental element of many biochemical reactions, has been studied intensively in solution. We report the results of electron transport (ETp) measurements across proteins, sandwiched between two…

介观与纳米尺度物理 · 物理学 2009-03-26 Izhar Ron , Lior Sepunaro , Stella Izhakov , Noga Friedman , Israel Pecht , Mordechai Sheves , David Cahen

Cryo-electron microscopy (cryo-EM) has revolutionized experimental protein structure determination. Despite advances in high resolution reconstruction, a majority of cryo-EM experiments provide either a single state of the studied…

In recent years, extracellular vesicles such have become promising carriers as the next-generation drug delivery platforms. Effective loading of exogenous cargos without compromising the extracellular vesicle membrane is a major challenge.…

生物大分子 · 定量生物学 2023-04-14 Khayrul Islam , Meghdad Razizadeh , Yaling Liu

Accurate event-based modeling of electric vehicle (EV) charging is essential for grid reliability and smart-charging design. While traditional statistical methods capture marginal distributions, they often fail to model the complex,…

机器学习 · 计算机科学 2026-04-01 Martin Výboh , Gabriela Grmanová

Introduction: The extracellular matrix (ECM) is a networkof proteins and carbohydrates that has a structural and bio-chemical function. The ECM plays an important role in dif-ferentiation, migration and signaling. Several studies…

定量方法 · 定量生物学 2022-02-17 Mohamed Ghafoor , Anh Nguyen

The endoplasmic reticulum (ER) is the largest continuous membrane-bound organelle in the cell and plays a central role in the synthesis and turnover of many lipids and proteins. It connects directly to the nucleus through specialized…

Proteins are macromolecules that perform essential functions in all living organisms. Designing novel proteins with specific structures and desired functions has been a long-standing challenge in the field of bioengineering. Existing…

生物大分子 · 定量生物学 2023-03-03 Chence Shi , Chuanrui Wang , Jiarui Lu , Bozitao Zhong , Jian Tang

Vibrational-electronic (vibronic) resonance and its role in energy and charge transfer has been experimentally and theoretically investigated in several photosynthetic proteins. Using a dimer modeled on a typical photosynthetic protein, we…

化学物理 · 物理学 2020-09-03 Amitav Sahu , Jo Sony Kurian , Vivek Tiwari

Extracellular vesicles (EVs) have drawn rapidly increasing attention as the next-generation diagnostic biomarkers and therapeutic agents. However, the heterogeneous nature of EVs necessitates advanced methods for profiling EVs at the…

生物大分子 · 定量生物学 2025-07-08 Yiting Liu , Anthony James El-helou , Bill Soderstrom , Juanfang Ruan , Ying Zhu

After the recent ground-breaking advances in protein structure prediction, one of the remaining challenges in protein machine learning is to reliably predict distributions of structural states. Parametric models of fluctuations are…

机器学习 · 计算机科学 2024-01-25 Marloes Arts , Jes Frellsen , Wouter Boomsma

Accurate characterization of extracellular vesicles (EVs) is critical to explore their diagnostic and therapeutic applications. As the EV research field has developed, so too have the techniques used to characterize them. The development of…

Directing extracellular vesicles (EVs), such as exosomes and microvesicles, toward specific cells is an emerging focus in nanomedicine, owing to their natural role as carriers of proteins, RNAs, and drugs. EVs can be manipulated by external…

Protein contacts contain important information for protein structure and functional study, but contact prediction from sequence information remains very challenging. Recently evolutionary coupling (EC) analysis, which predicts contacts by…

定量方法 · 定量生物学 2015-12-01 Siqi Sun , Jianzhu Ma , Sheng Wang , Jinbo Xu

Cryo-electron microscopy (cryo-EM) has recently become a premier method for obtaining high-resolution structures of biological macromolecules. However, it is limited to biomolecular samples with low conformational heterogeneity, where all…

The structure and function of biological molecules are strongly influenced by the water and dissolved ions that surround them. This aqueous solution (solvent) exerts significant electrostatic forces in response to the biomolecule's…

数值分析 · 数学 2015-12-29 Matthew G. Knepley , Jaydeep P. Bardhan

We suggest to augment standard isoelectronic focusing for separation of proteins in a gradient of pH by a similar focusing in the presence of a strongly charged polyelectrolyte (PE). Proteins which have the same isoelectric point but…

凝聚态物理 · 物理学 2009-11-07 E. M. Baskin , B. I. Shklovskii , G. V. Zilberstein

Protein complexes are macromolecules essential to the functioning and well-being of all living organisms. As the structure of a protein complex, in particular its region of interaction between multiple protein subunits (i.e., chains), has a…

机器学习 · 计算机科学 2022-05-26 Alex Morehead , Xiao Chen , Tianqi Wu , Jian Liu , Jianlin Cheng

Cellular heterogeneity is important to biological processes, including cancer and development. However, proteome heterogeneity is largely unexplored because of the limitations of existing methods for quantifying protein levels in single…

基因组学 · 定量生物学 2018-10-29 Bogdan Budnik , Ezra Levy , Guillaume Harmange , Nikolai Slavov

The endoplasmic reticulum (ER) is a dynamic network of interconnected sheets and tubules that orchestrates the distribution of lipids, ions, and proteins throughout the cell. The impact of its complex, dynamic morphology on its function as…

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