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Electron ptychography provides highly sensitive, dose efficient phase images which can be corrected for aberrations after the data has been acquired. This is crucial when very precise quantification is required, such as with sensitivity to…

Previous Zernike phase-plates, for transmission-electron-microscopy (TEM) have always been made out of carbon. A critical constraint on those phase-plates is their fast degradation during use. Here, a Zernike phase-plate consisting out of…

Flow cytometry mainly used for detecting the characteristics of a number of biochemical substances based on the expression of specific markers in cells. It is particularly useful for detecting membrane surface receptors, antigens, ions, or…

Flow cytometry is a technology that rapidly measures antigen-based markers associated to cells in a cell population. Although analysis of flow cytometry data has traditionally considered one or two markers at a time, there has been…

In Part I of this diptych, we outline the parallel mode of differential phase contrast (TEM-DPC), which uses real-space distortion of Fresnel images arising from electrostatic or magnetostatic fields to quantify the phase gradient of…

Computational cardiovascular flow modeling plays a crucial role in understanding blood flow dynamics. While 3D models provide acute details, they are computationally expensive, especially with fluid-structure interaction (FSI) simulations.…

Single-cell technologies have revolutionized biomedical research by enabling scalable measurement of the genome, transcriptome, and proteome of multiple systems at single-cell resolution. Now widely applied to cancer models, these assays…

Classical reconstruction methods for phase-contrast tomography consist of two stages: phase retrieval and tomographic reconstruction. A novel algebraic method combining the two was suggested by Kostenko et al. (Opt. Express, 21, 12185,…

Phase retrieval, i.e. the reconstruction of phase information from intensity information, is a central problem in many optical systems. Here, we demonstrate that a deep residual neural net is able to quickly and accurately perform this task…

In the last five decades, iterative phase retrieval methods draw large amount of interest across the research community as a non-interferometric approach to recover quantitative phase distributions from one (or more) intensity measurement.…

We present a wide-field multi-pass implementation of label-free imaging flow cytometry. Our technique is shown for high-speed flow imaging of ensembles of human red blood cells with up to four passes, demonstrating x4 enhancement in…

We show how the ability to slow down, amplify, and capture fast transient events can produce high-throughput real-time instruments ranging from digitizers to imaging flow cytometers for detection of rare diseased cells in blood.

At temperatures well above the transition, QCD admits a thermally reduced version in 3D, which reproduces the long distance physics. We analyze the phase diagram, point out the relevance of Z(3) symmetry in the location of the transition…

Flow cytometry is often used to characterize the malignant cells in leukemia and lymphoma patients, traced to the level of the individual cell. Typically, flow cytometric data analysis is performed through a series of 2-dimensional…

Fluorescence microscopy is an essential tool for the analysis of 3D subcellular structures in tissue. An important step in the characterization of tissue involves nuclei segmentation. In this paper, a two-stage method for segmentation of…

A unified method for three-dimensional reconstruction of objects from transmission images collected at multiple illumination directions is described. The method may be applicable to experimental conditions relevant to absorption-based,…

The Fourier inversion of phased coherent diffraction patterns offers images without the resolution and depth-of-focus limitations of lens-based tomographic systems. We report on our recent experimental images inverted using recent…

A fully automated system for interpreting abdominal computed tomography (CT) scans with multiple phases of contrast enhancement requires an accurate classification of the phases. This work aims at developing and validating a precise, fast…

Photon-Counting Computed Tomography (PCCT) is a novel imaging modality that simultaneously acquires volumetric data at multiple X-ray energy levels, generating separate volumes that capture energy-dependent attenuation properties.…

X-ray phase contrast imaging significantly improves the visualization of structures with weak or uniform absorption, broadening its applications across a wide range of scientific disciplines. Propagation-based phase contrast is particularly…