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The field of histology relies heavily on antiquated tissue processing and staining techniques that limit the efficiency of pathologic diagnoses of cancer and other diseases. Current staining and advanced labeling methods are often…

We report a novel lensless on-chip microscopy platform based on near-field blind ptychographic modulation. In this platform, we place a thin diffuser in between the object and the image sensor for light wave modulation. By blindly scanning…

图像与视频处理 · 电气工程与系统科学 2020-02-13 Shaowei Jiang , Jiakai Zhu , Pengming Song , Chengfei Guo , Zichao Bian , Ruihai Wang , Yikun Huang , Shiyao Wang , He Zhang , Guoan Zheng

We report an angle-tilted, wavelength-multiplexed ptychographic modulation approach for multispectral lensless on-chip microscopy. In this approach, we illuminate the specimen with lights at 5 wavelengths simultaneously. A prism is added at…

We report a compact, cost-effective and field-portable lensless imaging platform for quantitative microscopy. In this platform, the object is placed on top of an image sensor chip without using any lens. We use a low-cost galvo scanner to…

图像与视频处理 · 电气工程与系统科学 2019-04-26 He Zhang , Zichao Bian , Shaowei Jiang , Jian Liu , Pengming Song , Guoan Zheng

Ptychography is an enabling coherent diffraction imaging technique for both fundamental and applied sciences. Its applications in optical microscopy, however, fall short for its low imaging throughput and limited resolution. Here, we report…

Ptychography has become prominent at synchrotron facilities worldwide for characterizing biological and material specimens' topological structures and properties at the nanometer or atomic scale, due to its lens - less, highly quantitative…

图像与视频处理 · 电气工程与系统科学 2025-01-28 Tao Liu , Bingyang Wang , JiangTao Zhao , Maik Kahnt , Fucai Zhang

Whole slide imaging provides a wide field-of-view (FOV) across cross-sections of biopsy or surgery samples, significantly facilitating pathological analysis and clinical diagnosis. Such high-quality images that enable detailed visualization…

光学 · 物理学 2024-07-31 Shi Zhao , Haowen Zhou , Siyu Lin , Ruizhi Cao , Changhuei Yang

We demonstrate a motion-free intensity diffraction tomography technique that enables direct inversion of 3D phase and absorption from intensity-only measurements for weakly scattering samples. We derive a novel linear forward model,…

图像与视频处理 · 电气工程与系统科学 2018-06-26 Ruilong Ling , Waleed Tahir , Hsing-Ying Lin , Hakho Lee , Lei Tian

We report the implementation of a fully on-chip, lensless microscopy technique termed optofluidic ptychography. This imaging modality complements the miniaturization provided by microfluidics and allows the integration of ptychographic…

仪器与探测器 · 物理学 2021-12-21 Pengming Song , Chengfei Guo , Shaowei Jiang , Tianbo Wang , Patrick Hu , Derek Hu , Zibang Zhang , Bin Feng , Guoan Zheng

Capturing biological specimens at large scales with sub-micron resolution is crucial for biomedical research, but conventional cameras often can't handle the pixel requirements. While most microscopes use motorized stages to move samples…

光学 · 物理学 2025-03-28 Charlie Kersuzan , Aymerick Bazin , Amaury Badon

Background: The use of whole slide imaging (WSI) for digital pathology has recently been cleared for primary diagnosis in the US. A conventional WSI system scans the tissue slide to different positions and acquires the digital images. In a…

图像与视频处理 · 电气工程与系统科学 2019-07-26 Shaowei Jiang , Zichao Bian , Xizhi Huang , Pengming Song , He Zhang , Yongbing Zhang , Guoan Zheng

Blu-ray drive is an engineering masterpiece that integrates disc rotation, pickup head translation, and three lasers in a compact and portable format. Here we integrate a blood-coated image sensor with a modified Blu-ray drive for…

仪器与探测器 · 物理学 2022-10-28 Shaowei Jiang , Chengfei Guo , Tianbo Wang , Jia Liu , Pengming Song , Terrance Zhang , Ruihai Wang , Bin Feng , Guoan Zheng

Ptychography is a lensless, computational imaging method that utilises diffraction patterns to determine the amplitude and phase of an object. In transmission ptychography, the diffraction patterns are recorded by a detector positioned…

图像与视频处理 · 电气工程与系统科学 2023-01-04 Kira A. M. Maathuis , Jacob Seifert , Allard P. Mosk

We report the development of a multichannel microscopy for whole-slide multiplane, multispectral, and phase imaging. We use trinocular heads to split the beam path into 6 independent channels and employ a camera array for parallel data…

Imaging specimens over large scales and with a sub-micron resolution is instrumental to biomedical research. Yet, the number of pixels to form such an image usually exceeds the number of pixels provided by conventional cameras. While most…

仪器与探测器 · 物理学 2019-12-03 Gaelle Recher , Pierre Nassoy , Amaury Badon

Lensless imaging is a popular research field for the advantages of small size, wide field-of-view and low aberration in recent years. However, some traditional lensless imaging methods suffer from slow convergence, mechanical errors and…

Traditional microbial detection methods often rely on the overall property of microbial cultures and cannot resolve individual growth event at high spatiotemporal resolution. As a result, they require bacteria to grow to confluence and then…

We report a new coherent imaging technique, termed ptychographic structured modulation (PSM), for quantitative super-resolution microscopy. In this technique, we place a thin diffuser (i.e., a scattering lens) in between the sample and the…

图像与视频处理 · 电气工程与系统科学 2019-07-26 Pengming Song , Shaowei Jiang , He Zhang , Zichao Bian , Chengfei Guo , Kazunori Hoshino , Guoan Zheng

Coherent diffractive imaging (CDI) enables lensless imaging with experimental simplicity and a flexible field of view, yet its resolution is fundamentally constrained by the Abbe diffraction limit. To overcome this limitation, we introduce…

We describe a full-field coherent imaging approach suitable for hard X-rays based on a classical (i.e. Galilean) X-ray microscope. The method combines a series of low-resolution images acquired at different transverse lens positions into a…

光学 · 物理学 2019-05-07 Carsten Detlefs , Mario A. Beltran , Jean-Pierre Guigay , Hugh Simons
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