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Metagenomics sequencing is routinely applied to quantify bacterial abundances in microbiome studies, where the bacterial composition is estimated based on the sequencing read counts. Due to limited sequencing depth and DNA dropouts, many…

统计方法学 · 统计学 2019-04-26 Yuanpei Cao , Anru Zhang , Hongzhe Li

The ability to quickly and accurately identify microbial species in a sample, known as metagenomic profiling, is critical across various fields, from healthcare to environmental science. This paper introduces a novel method to profile…

基因组学 · 定量生物学 2025-04-10 Riselda Kodra , Hadjer Benmeziane , Irem Boybat , William Andrew Simon

As sequencing technologies become more affordable and genomic databases expand continuously, the reuse of publicly available sequencing data emerges as a powerful strategy for studying microbial pathogens. Indeed, raw sequencing reads…

定量方法 · 定量生物学 2025-05-16 Damien Richard , Nils Poulicard

Background: The short reads output by first- and second-generation DNA sequencing instruments cannot completely reconstruct microbial chromosomes. Therefore, most genomes have been left unfinished due to the significant resources required…

Microbiome sample representation to input into LLMs is essential for downstream tasks such as phenotype prediction and environmental classification. While prior studies have explored embedding-based representations of each microbiome…

机器学习 · 计算机科学 2025-08-18 Hyunwoo Yoo , Gail Rosen

Microorganisms can create engineered materials with exquisite structures and living functionalities. Although synthetic biology tools to genetically manipulate microorganisms continue to expand, the bottom-up rational design of engineered…

Minimizing data storage poses a significant challenge in large-scale metagenomic projects. In this paper, we present a new method for improving the encoding of FASTQ files generated by metagenomic sequencing. This method incorporates…

信息论 · 计算机科学 2024-01-04 Diogo Pratas , Armando J. Pinho

Microbes are essentially yet convolutedly linked with human lives on the earth. They critically interfere in different physiological processes and thus influence overall health status. Studying microbial species is used to be constrained to…

基因组学 · 定量生物学 2021-09-03 Chao Yang , Debajyoti Chowdhury , Zhenmiao Zhang , William K. Cheung , Aiping Lu , Zhao Xiang Bian , Lu Zhang

Droplet microfluidics offers a versatile platform for analyzing liquid samples. Despite its potential, there is a lack of techniques that allow to reliably probe individual circulating droplets. The prospect of combining droplet…

定量方法 · 定量生物学 2024-03-08 Marc Sulliger , Jaime Ortega Arroyo , Romain Quidant

Metagenomics has lowered the barrier to microbial discovery--enabling the identification of novel microbes without isolation--but cultures remain imperative for the deep study of microbes. Cultivation and isolation of non-model microbes…

分子网络 · 定量生物学 2026-02-26 Olivia Bulka , Chabname Ghassemi Nedjad , Loïc Paulevé , Sylvain Prigent , Clémence Frioux

Over the recent years, the field of whole metagenome shotgun sequencing has witnessed significant growth due to the high-throughput sequencing technologies that allow sequencing genomic samples cheaper, faster, and with better coverage than…

基因组学 · 定量生物学 2014-04-04 Sohan Seth , Niko Välimäki , Samuel Kaski , Antti Honkela

Uncovering the heterogeneity of cell populations is a long-standing goal in fields ranging from antimicrobial resistance to cancer research. Emerging technology platforms such as droplet microfluidics hold the promise to decipher cellular…

图像与视频处理 · 电气工程与系统科学 2020-01-29 Vasileios Anagnostidis , Benjamin Sherlock , Jeremy Metz , Philip Mair , Florian Hollfelder , Fabrice Gielen

Studying the human microbiome has gained substantial interest in recent years, and a common task in the analysis of these data is to cluster microbiome compositions into subtypes. This subdivision of samples into subgroups serves as an…

统计方法学 · 统计学 2020-10-22 Jialiang Mao , Li Ma

Ancient mitochondrial DNA has been used in a wide variety of palaeontological and archaeological studies, ranging from population dynamics of extinct species to patterns of domestication. Most of these studies have traditionally been based…

基因组学 · 定量生物学 2016-04-26 Gloria G. Fortes , Johanna L. A. Paijmans

Nutrient gradients and limitations play a pivotal role in the life of all microbes, both in their natural habitat as well as in artificial, microfluidic systems. Spatial concentration gradients of nutrients in densely packed cell…

Microbiome research has immense potential for unlocking insights into human health and disease. A common goal in human microbiome research is identifying subgroups of individuals with similar microbial composition that may be linked to…

统计方法学 · 统计学 2025-08-21 Suppapat Korsurat , Matthew D. Koslovsky

We introduce InVirtuoGen, a discrete flow generative model for fragmented SMILES for de novo and fragment-constrained generation, and target-property/lead optimization of small molecules. The model learns to transform a uniform source over…

机器学习 · 计算机科学 2025-10-01 Benno Kaech , Luis Wyss , Karsten Borgwardt , Gianvito Grasso

We developed a low-cost, high-throughput microbiome profiling method that uses combinatorial sequence tags attached to PCR primers that amplify the rRNA V6 region. Amplified PCR products are sequenced using an Illumina paired-end protocol…

High-throughput sequencing technology provides unprecedented opportunities to quantitatively explore human gut microbiome and its relation to diseases. Microbiome data are compositional, sparse, noisy, and heterogeneous, which pose serious…

统计方法学 · 统计学 2020-10-12 Fangting Zhou , Kejun He , Qiwei Li , Robert S. Chapkin , Yang Ni

Metagenomic disease prediction commonly relies on species abundance tables derived from large, incomplete reference catalogs, constraining resolution and discarding valuable information contained in DNA reads. To overcome these limitations,…

基因组学 · 定量生物学 2026-01-08 Gaspar Roy , Eugeni Belda , Baptiste Hennecart , Yann Chevaleyre , Edi Prifti , Jean-Daniel Zucker
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