相关论文: Axially Overlapped Multi-Focus Light Sheet with En…
Multifocus microscopy enables recording of entire volumes in a single camera exposure. In dense samples, multifocus microscopy is severely hampered by background haze. Here, we introduce a scalable multifocus method that incorporates…
Oblique plane microscopy is a method enabling light-sheet fluorescence imaging through a single microscope objective lens by focusing on a tilted plane within the sample. To focus the fluorescence emitted by the oblique plane on a camera,…
High resolution light-sheet optical microscopy requires illuminating a sample by a thin excitation light sheet with large area. Here, we describe methods for producing light sheets with extended length, as compared to Gaussian-beam-type…
Wide-field fluorescence microscopy, while much faster than confocal microscopy, suffers from a lack of optical sectioning and poor axial resolution. 3D structured illumination microscopy (SIM) has been demonstrated to provide optical…
We developed a Bessel light sheet fluorescence microscopy (LSFM) system to enable high-speed, wide-field intra-vital imaging of zebrafish and other thick biological samples. This system uses air objectives for the convenient mounting of…
Light sheet fluorescence microscopy is able to image large specimen with high resolution by imaging the sam- ples from multiple angles. Multi-view deconvolution can significantly improve the resolution and contrast of the images, but its…
Light field microscopy (LFM) uses a microlens array (MLA) near the sensor plane of a microscope to achieve single-shot 3D imaging of a sample without any moving parts. Unfortunately, the 3D capability of LFM comes with a significant loss of…
Evanescent-wave microscopy achieves sub-diffraction axial sectioning by confining fluorescence excitation to a thin layer close to the cell/substrate interface. How thin this light sheet exactly is, however, is often unknown. Particularly…
A fluorescence correlation spectroscopy (FCS) system based on two independent measurement volumes is presented. The optical setup and data acquisition hardware are detailed, as well as a complete protocol to control the location, size and…
We demonstrate the first planar Airy light-sheet microscope. Fluorescence light-sheet microscopy has become the method of choice to study large biological samples with cellular or sub-cellular resolution. The propagation-invariant Airy beam…
We studied both theoretically and experimentally the intensity distribution of a Gaussian laser beam when it was focussed by an objective lens with its numerical-aperture (NA) up to 0.95. Approximate formulae for full widths at half maximum…
The possibility of focusing light to an ever tighter spot has important implications for many applications and fields of optics research, such as nano-optics and plasmonics, laser-scanning microscopy, optical data storage and many more. The…
In this report, we demonstrate a new principle to improve the resolution of the acoustic microscopy, which is based on the sub-wavelength focusing of acoustic wave passing through an acoustically transparent mesoscale particle. In the…
Oblique plane microscopy (OPM) offers 3D optically sectioned imaging with high spatial- and temporal-resolution while enabling conventional sample mounting. The technique uses a concatenation of three microscopes, two for remote focusing…
Scattering and absorption limit the penetration of optical fields into tissue, but wavefront correction, often used to compensate for these effects, is incompatible with wide field-of-view imaging and complex to implement. We demonstrate a…
Phase Fresnel lenses have the same imaging properties as zone plates, but with the possibility of concentrating all of the incident power into the primary focus, increasing the maximum theoretical efficiency from 11% to close to 100%. For…
Current optoacoustic microscopy configurations often have narrow focal ranges that limit their use for fast volumetric imaging applications. Here, the focal range of optoacoustic microscopes is extended by matching the elongated optical…
Light field (LF) imaging captures both angular and spatial light distributions, enabling advanced photographic techniques. However, micro-lens array (MLA)- based cameras face a spatial-angular resolution tradeoff due to a single shared…
Most research on astrophysical lensing has been conducted using the geometric optics framework, where there exists a clear concept of lensing images. However, wave optics effects can be important for coherent sources, e.g. pulsars, fast…
We investigate lensless endoscopy using coherent beam combining and aperiodic multicore fibers (MCF). We show that diffracted orders, inherent to MCF with periodically arranged cores, dramatically reduce the field of view (FoV) and that…