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A variety of recent imaging techniques are able to beat the diffraction limit in fluorescence microcopy by activating and localizing subsets of the fluorescent molecules in the specimen, and repeating this process until all of the molecules…

光学 · 物理学 2010-08-27 Alexander R. Small

In deep tissue photoacoustic imaging the spatial resolution is inherently limited by the acoustic wavelength. We present an approach for surpassing the acoustic diffraction limit by exploiting temporal fluctuations in the sample absorption…

光学 · 物理学 2017-05-31 Thomas Chaigne , Bastien Arnal , Sergey Vilov , Emmanuel Bossy , Ori Katz

Super-resolution microscopy is crucial for imaging sub-wavelength biological structures. However, most techniques rely on nonlinear saturation or stochastic switching of emitters, limiting imaging speed and increasing phototoxicity. Here,…

The recent use of diffusion prior, enhanced by pre-trained text-image models, has markedly elevated the performance of image super-resolution (SR). To alleviate the huge computational cost required by pixel-based diffusion SR, latent-based…

计算机视觉与模式识别 · 计算机科学 2023-12-14 Feng Luo , Jinxi Xiang , Jun Zhang , Xiao Han , Wei Yang

We propose a method of spatial resolution enhancement in metrology (thermometry, magnetometry, pH estimation, and similar methods) with blinking fluorescent nanosensors by combining sensing with super-resolution optical fluctuation imaging…

光学 · 物理学 2026-05-11 Alexander Mikhalychev , Alex Ulyanenkov

Intensity correlation microscopy (ICM), which is prominently known through antibunching microscopy or super-resolution optical fluctuation imaging (SOFI), provides superresolution through a correlation analysis of antibunching of…

光学 · 物理学 2018-10-08 Anton Classen , Joachim von Zanthier , Girish S. Agarwal

Recently, sparsity-based algorithms are proposed for super-resolution spectrum estimation. However, to achieve adequately high resolution in real-world signal analysis, the dictionary atoms have to be close to each other in frequency,…

机器学习 · 统计学 2015-06-05 Yiyuan She , Huanghuang Li , Jiangping Wang , Dapeng Wu

Most existing super-resolution methods and datasets have been developed to improve the image quality in well-lighted conditions. However, these methods do not work well in real-world low-light conditions as the images captured in such…

计算机视觉与模式识别 · 计算机科学 2024-10-18 Yang Liu , Yaofang Liu , Jinshan Pan , Yuxiang Hui , Fan Jia , Raymond H. Chan , Tieyong Zeng

Electrochromic optical recording (ECORE) is a label-free method that utilizes electrochromism to optically detect electrical signals in biological cells with a high signal-to-noise ratio and is suitable for long-term recording. However,…

Resolution enhancements are often desired in imaging applications where high-resolution sensor arrays are difficult to obtain. Many computational imaging methods have been proposed to encode high-resolution scene information on…

图像与视频处理 · 电气工程与系统科学 2018-10-29 Kevin Beale , Jianbo Chen , Kevin F. Kelly , Justin Romberg

The proliferation of microscopy methods for live-cell imaging offers many new possibilities for users but can also be challenging to navigate. We focus here on computational methods that promise to boost live-cell fluorescence microscopy,…

定量方法 · 定量生物学 2024-01-04 Hari Shroff , Ilaria Testa , Florian Jug , Suliana Manley

Coherent Diffractive Imaging is a lensless technique that allows imaging of matter at a spatial resolution not limited by lens aberrations. This technique exploits the measured diffraction pattern of a coherent beam scattered by periodic…

Confocal microscopy has long been a cornerstone technique for visualizing complex interactions and processes within cellular structures. However, achieving super-resolution imaging of multiple organelles and their interactions…

光学 · 物理学 2025-08-19 Qinglin Chen , Luwei Wang , Jia Li , Dan Shao , Xiaoyu Weng , Liwei Liu , Dayong Jin , Junle Qu

Proposals to determine biomolecular structures from diffraction experiments using femtosecond X-ray free-electron laser (XFEL) pulses involve a conflict between the incident brightness required to achieve diffraction-limited atomic…

光学 · 物理学 2015-05-19 Harry M. Quiney , Keith A. Nugent

We consider imaging of two partially coherent sources and derive the ultimate quantum limits for estimating the separation, location, relative intensity, and coherence factor. We show that super-resolution in the separation is achievable…

量子物理 · 物理学 2026-05-20 Joaquín López-Suárez , Michalis Skotiniotis

Fluorescence microscopy allows for a detailed inspection of cells, cellular networks, and anatomical landmarks by staining with a variety of carefully-selected markers visualized as color channels. Quantitative characterization of…

计算机视觉与模式识别 · 计算机科学 2021-08-26 Alvaro Gomariz , Tiziano Portenier , Patrick M. Helbling , Stephan Isringhausen , Ute Suessbier , César Nombela-Arrieta , Orcun Goksel

An important topic of interest in imaging is the construction of protocols that are not diffraction limited. This can be achieved in a variety of ways, including classical superresolution techniques or quantum entanglement-based protocols.…

量子物理 · 物理学 2015-10-28 Mark E. Pearce , Thomas Mehringer , J. von Zanthier , Pieter Kok

Rapid growth in the field of quantitative digital image analysis is paving the way for researchers to make precise measurements about objects in an image. To compute quantities from the image such as the density of compressed materials or…

计算机视觉与模式识别 · 计算机科学 2017-07-31 Marylesa Howard , Margaret C. Hock , B. T. Meehan , Leora Dresselhaus-Cooper

A new tool providing material contrast control in scanning electron microscopy (SEM) is demonstrated. The approach is based on deep-UV illumination during SEM imaging and delivers a novel material based contrast as well as higher resolution…

仪器与探测器 · 物理学 2016-04-07 Gediminas Seniutinas , Armandas Balcytis , Saulius Juodkazis

Interferometric methods, renowned for their reliability and precision, play a vital role in phase imaging. Interferometry typically requires high coherence and stability between the measured and the reference beam. The presence of rapid…