相关论文: Holographic immunoassays
Holographic molecular binding assays detect macromolecules binding to colloidal probe beads by monitoring nanometer-scale changes in the beads' diameters with holographic microscopy. Measured changes are interpreted with Maxwell Garnett…
Holographic molecular binding assays use holographic video microscopy to directly detect molecules binding to the surfaces of micrometer-scale colloidal beads by monitoring associated changes in the beads' light-scattering properties.…
This study introduces a Holographic Agglutination Assay for quantifying levels of the immunoglobulin protein IgA in biological samples. This is the first example of a label-free and bead-free assay that quantifies protein agglutinates by…
We demonstrate the use of holographic video microscopy to detect individual subvisible particles dispersed in biopharmaceutical formulations and to differentiate them based on material characteristics measured from their holograms. The…
Fiber imaging bundles are widely used as thin, passive image conduits for miniaturised and endoscopic microscopy, particularly for confocal fluorescence imaging. Holographic microscopy through fiber bundles is more challenging; phase…
Multiplexed, specific and sensitive detection of antigens is critical for the rapid and accurate diagnosis of disease and the informed development of personalized treatment plans. Here, we show that polymer microsphere lasers can be used as…
IR absorption spectroscopy of hemoglobin was performed using an IR optical parametric oscillator laser and a commercial atomic force microscope in a novel experimental arrangement based on the use of a bottom-up excitation alignment. This…
Colloidal spheres synthesized from polymer gels swell by absorbing molecules from solution. The resulting change in size can be monitored with nanometer precision using holographic video microscopy. When the absorbate is chemically similar…
Holographic particle characterization uses quantitative analysis of holographic microscopy data to precisely and rapidly measure the diameter and refractive index of individual colloidal spheres in their native media. When this technique is…
Holographic coherent X-ray imaging enables nanoscale imaging of biological cells and tissues, rendering both phase and absorption contrast, i.e. real and imaginary parts of the refractive index. Unlike the standard model, which assumes a…
Size and shape are critical discriminators between molecular species and states. We describe a micro-chip based high-throughput imaging approach offering rapid and precise determination of molecular properties under native solution…
We demonstrate precise measurements of the size and refractive index of individual dimpled colloidal spheres using holographic characterization techniques developed for ideal spheres.
Particle agglutination assays are widely adapted immunological tests that are based on antigen-antibody interactions. Antibody-coated microscopic particles are mixed with a test sample that potentially contains the target antigen, as a…
Holography is an established technique for measuring the wavefront of optical signals through interferometric combination with a reference wave. Conventionally the integration time of a hologram is limited by the interferometer coherence…
In the last fifteen years several techniques based on the holographic principle have been developed for the study of the 3D local order in solids. These methods use various particles: electrons, hard x-ray photons, gamma photons, or…
We introduce a number of techniques in quantitative convergent-beam electron diffraction under development by our group and discuss the basis for measuring interatomic electrostatic potentials (and therefore also electron densities),…
We present a new and versatile implementation of rapid and localized immunohistochemical staining of tissue sections. Immunohistochemistry (IHC) allows to detect specific proteins on tissue sections and comprises a sequence of specific…
We use digital holographic microscopy and Mie scattering theory to simultaneously characterize and track individual colloidal particles. Each holographic snapshot provides enough information to measure a colloidal sphere's radius and…
Digital holographic microscopy is a thriving imaging modality that attracted considerable research interest in quantitative biological cell imaging due to its ability to not only create excellent label-free contrast, but also supply…
Molecular adhesion plays a central role in many biological systems, yet existing methods to quantify adhesive strength often struggle to bridge the gap between single-molecule resolution and biologically relevant environments. Here, we…