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We present a theoretical evaluation of radiation dose constraints for extreme ultraviolet (EUV) and soft X-ray microscopy. Our work particularly addresses the long-standing concern regarding strong absorption of EUV radiation in biological…

应用物理 · 物理学 2026-05-29 Chang Liu , Leona Licht , Jan Rothhardt

X-ray diffraction microscopy (XDM) is a new form of x-ray imaging that is being practiced at several third-generation synchrotron-radiation x-ray facilities. Although only five years have elapsed since the technique was first introduced, it…

This paper describes the setup of two different solutions for laboratory X-ray microscopy working with geometric magnification. One setup uses thin-film transmission targets with an optimized tungsten-layer thickness and the electron gun…

仪器与探测器 · 物理学 2015-06-11 Thomas Ebensperger , Philipp Stahlhut , Frank Nachtrab , Simon Zabler , Randolf Hanke

The challenge of imaging low-density objects in an electron microscope without causing beam damage is significant in modern TEM. This is especially true for life science imaging, where the sample, rather than the instrument, still…

光学 · 物理学 2025-01-29 Francisco Vega Ibáñez , Jo Verbeeck

Transmission electron microscopes use electrons with wavelengths of a few picometers, potentially capable of imaging individual atoms in solids at a resolution ultimately set by the intrinsic size of an atom. Unfortunately, due to…

Electron ptychography describes a family of algorithms which are used to enable the reconstruction of complex specimen transmission functions of a sample in order to obtain both phase and amplitude information, as applied within the realms…

光学 · 物理学 2025-03-17 L. Clark , P. D. Nellist

There is a clear need for developments in characterisation techniques that provide detailed information about structure-function relationships in biology. Using electron microscopy to achieve high resolution while maintaining a broad field…

定量方法 · 定量生物学 2023-09-12 Chen Huang , Judy S. Kim , Angus I. Kirkland

This paper is concerned with investigating super-resolution algorithms and solutions for handling electron microscopic images. We note two main aspects differentiating the problem discussed here from those considered in the literature. The…

图像与视频处理 · 电气工程与系统科学 2020-08-26 Yanjun Qian , Jiaxi Xu , Lawrence F. Drummy , Yu Ding

Modern microscopy techniques are aimed at imaging an individual molecule at atomic resolution. Here we show that low-energy electrons with kinetic energies of 50-250 eV offer a possibility of overcome the problem of radiation damage, and…

生物物理 · 物理学 2013-05-09 Tatiana Latychevskaia , Jean-Nicolas Longchamp , Conrad Escher , Hans-Werner Fink

We show that radiation damage to unstained biological specimens is not an intractable problem in electron microscopy. When a structural hypothesis of a specimen is available, quantum mechanical principles allow us to verify the hypothesis…

量子物理 · 物理学 2015-07-01 Hiroshi Okamoto , Tatiana Latychevskaia , Hans-Werner Fink

A number of visions for a new generation of dose-efficient electron microscopes have been advanced. These proposals, while inspired by quantum principles, make little contact with the broader field of quantum metrology. We discuss a…

Structural studies of biological macromolecules are severely limited by radiation damage. Traditional crystallography curbs the effects of damage by spreading damage over many copies of the molecule of interest. X-ray lasers, such as the…

Defects in crystalline materials control the properties of materials, and their characterization focuses our strategies to optimize performance. Electron microscopy has served as the backbone of our understanding of defect structure and…

材料科学 · 物理学 2019-03-19 Daniel S. Gianola , T. Ben Britton , Stefan Zaefferer

Electron ptychography has seen a recent surge of interest for phase sensitive imaging at atomic or near-atomic resolution. However, applications are so far mainly limited to radiation-hard samples because the required doses are too high for…

Electron microscopy is a powerful tool for studying the properties of materials down to their atomic structure. In many cases, the quantitative interpretation of images requires simulations based on atomistic structure models. These…

Size effects are ubiquitous in the structural, mechanical, and physical properties of materials, making it highly desirable to study the intrinsic properties of thick objects through high-resolution structural analysis in transmission…

材料科学 · 物理学 2025-02-26 Wenfeng Yang , Haozhi Sha , Jizhe Cui , Rong Yu

While electron microscopy offers crucial atomic-resolution insights into structure-property relationships, radiation damage severely limits its use on beam-sensitive materials like proteins and 2D materials. To overcome this challenge, we…

计算机视觉与模式识别 · 计算机科学 2025-07-18 Zifei Wang , Zian Mao , Xiaoya He , Xi Huang , Haoran Zhang , Chun Cheng , Shufen Chu , Tingzheng Hou , Xiaoqin Zeng , Yujun Xie

Aberration-corrected electron microscopy can resolve the smallest atomic bond-lengths in nature. However, the high-convergence angles that enable spectacular resolution in 2D have unknown 3D resolution limits for all but the smallest…

材料科学 · 物理学 2021-01-13 Reed Yalisove , Suk Hyun Sung , Peter Ercius , Robert Hovden

Feynman once asked physicists to build better electron microscopes to be able to watch biology at work. While electron microscopes can now provide atomic resolution, electron beam induced specimen damage precludes high resolution imaging of…

The goal of this study is to estimate the amount of lost data in electron microscopy and to analyze the extent to which experimentally acquired images are utilized in peer-reviewed scientific publications. Analysis of the number of images…

数据库 · 计算机科学 2025-10-02 Nina M. Ivanova , Alexey S. Kashin , Valentine P. Ananikov
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