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Optical diffraction tomography (ODT) is a three-dimensional (3D) label-free imaging technique. The 3D refractive index distribution of a sample can be reconstructed from multiple two-dimensional optical field images via ODT. Herein, we…

光学 · 物理学 2021-02-03 Chansuk Park , Kyeoreh Lee , Yoonseok Baek , Yongkeun Park

Fluorescent imaging plays a critical role in a myriad of scientific endeavors, particularly in the biological sciences. Three-dimensional imaging of fluorescent intensity often requires serial data acquisition, that is voxel-by-voxel…

光学 · 物理学 2015-10-27 Jeffrey J. Field , David G. Winters , Randy A. Bartels

Optical diffraction tomography (ODT), initially described in the seminal paper of Emil Wolf [Opt. Comm., 1(4), 153-156 (1969)], has received renewed attention recently. The vast majority of ODT experiments reported to date have been 3D…

Simultaneous imaging of fluorescence-labeled and label-free phase objects in the same sample provides distinct and complementary information. Most multimodal fluorescence-phase imaging operates in transmission mode, capturing fluorescence…

光学 · 物理学 2024-08-20 Renzhi He , Yucheng Li , Junjie Chen , Yi Xue

Holographic Tomography (HT) or Optical Diffraction Tomography (ODT) provides slice-by-slice information about the refractive index (RI) of three-dimensional (3D) samples and is emerging as an important label-free imaging modality for Life…

图像与视频处理 · 电气工程与系统科学 2025-04-22 N Goyal , K Khare

In optical diffraction tomography (ODT), the three-dimensional scattering potential of a microscopic object rotating around its center is recovered by a series of illuminations with coherent light. Reconstruction algorithms such as the…

数值分析 · 数学 2022-09-02 Robert Beinert , Michael Quellmalz

This work introduces a novel reinterpretation of structured illumination (SI) microscopy for coherent imaging that allows three-dimensional imaging of complex refractive index (RI). To do so, we show that coherent SI is mathematically…

光学 · 物理学 2017-05-11 Shwetadwip Chowdhury , Will J. Eldridge , Adam Wax , Joseph A. Izatt

Optical diffraction tomography is an indispensable tool for studying objects in three-dimensions due to its ability to accurately reconstruct scattering objects. Until now this technique has been limited to coherent light because spatial…

The Diffuse Optical Tomography (DOT) has received considerable attention in the recent years in the field of biomedical imaging and disease detection. However, imaging through highly diffusive medium is a challenge and stability is always…

图像与视频处理 · 电气工程与系统科学 2020-03-31 Tapan Das , P. K. Dutta

Optical diffraction tomography (ODT) enables non-invasive information-rich 3D refractive index (RI) reconstruction of unimpaired transparent biological and technical samples, crucial in biomedical research, optical metrology, materials…

Optical Deflectometric Tomography (ODT) provides an accurate characterization of transparent materials whose complex surfaces present a real challenge for manufacture and control. In ODT, the refractive index map (RIM) of a transparent…

计算机视觉与模式识别 · 计算机科学 2013-10-31 Adriana Gonzalez , Laurent Jacques , Christophe De Vleeschouwer , Philippe Antoine

Tomography is the three-dimensional reconstruction of an object from images taken at different angles. The term classical tomography is used, when the imaging beam travels in straight lines through the object. This assumption is valid for…

定量方法 · 定量生物学 2016-10-10 Paul Müller , Mirjam Schürmann , Jochen Guck

Optical diffraction tomography (ODT) is a three-dimensional (3D) quantitative phase imaging technique, which enables the reconstruction of the 3D refractive index (RI) distribution of a transparent sample. Due to its fast, non-invasive, and…

光学 · 物理学 2018-11-14 Chansuk Park , Seungwoo Shin , Yongkeun Park

Recent advances in the field of attosecond science hold the promise of tracking electronic processes at the shortest space and time scales. Imaging methods that combine attosecond temporal with nanometer spatial resolution are currently out…

Wireless data transmission systems such as WiFi or Bluetooth emit coherent light - electromagnetic waves with precisely known amplitude and phase. Propagating in space, this radiation forms a hologram - a two-dimensional wavefront encoding…

经典物理 · 物理学 2017-05-10 Philipp Holl , Friedemann Reinhard

A unified method for three-dimensional reconstruction of objects from transmission images collected at multiple illumination directions is described. The method may be applicable to experimental conditions relevant to absorption-based,…

医学物理 · 物理学 2022-12-07 Timur E. Gureyev , Hamish G. Brown , Harry M. Quiney , Leslie J. Allen

Optical diffraction tomography (ODT) has emerged as a powerful label-free three-dimensional (3D) bioimaging techniques for observing living cells and thin tissue layers. We report a new reflection-mode ODT (rODT) method for imaging thick…

Ultrathin flat meta-optics have shown great promise for holography in recent years. However, most of the reported meta-optical holograms rely on only phase modulation and neglect the amplitude information. Modulation of both amplitude and…

Optical diffraction tomography (ODT) is a powerful non-invasive 3D imaging technique, but its combination with broadband light sources is difficult. In this study, we introduce ultrabroadband ODT, covering over 150 nm of visible spectral…

Diffraction tomography (DT) has been widely explored in transmission-mode configurations, enabling high-resolution, label-free 3D imaging. However, industrial metrology applications, such as semiconductor inspection, typically involve…

光学 · 物理学 2026-02-04 Jiabei Zhu , Tongyu Li , Hao Wang , Yi Shen , Guorong Hu , Lei Tian
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