相关论文: Spinning Disk -- Remote Focusing Microscopy

We analyse the global structure of the old stellar discs in 34 edge-on spiral galaxies. The radial and vertical exponential scale parameters of the discs are obtained by applying an improved two-dimensional decomposition technique to our…

Confining light illumination in the three dimensions of space is a challenge for various applications. Among these, optogenetic methods developed for live experiments in cell biology would benefit from such a localized illumination as it…

The vertebral levels of the spine provide a useful coordinate system when making measurements of plaque, muscle, fat, and bone mineral density. Correctly classifying vertebral levels with high accuracy is challenging due to the similar…

Diffusion magnetic resonance imaging (dMRI) is a crucial non-invasive technique for exploring the microstructure of the living human brain. Traditional hand-crafted and model-based tissue microstructure reconstruction methods often require…

Three-dimensional (3D) ultrasound imaging can overcome the limitations of conventional two dimensional (2D) ultrasound imaging in structural observation and measurement. However, conducting volumetric ultrasound imaging for large-sized…

We present a physics-informed deep learning framework to address common limitations in Confocal Laser Scanning Microscopy (CLSM), such as diffraction limited resolution, noise, and undersampling due to low laser power conditions. The…

We demonstrate a deep learning-based offline autofocusing method, termed Deep-R, that is trained to rapidly and blindly autofocus a single-shot microscopy image of a specimen that is acquired at an arbitrary out-of-focus plane. We…

Fluorescence Microcopy Calcium Imaging is a fundamental tool to in-vivo record and analyze large scale neuronal activities simultaneously at a single cell resolution. Automatic and precise detection of behaviorally relevant neuron activity…

We propose a robust image enhancement algorithm dedicated for muscle fiber specimen images captured by optical microscopes. Blur or out of focus problems are prevalent in muscle images during the image acquisition stage. Traditional image…

Attention is a powerful concept in computer vision. End-to-end networks that learn to focus selectively on regions of an image or video often perform strongly. However, other image regions, while not necessarily containing the signal of…

Fourier ptychography microscopy (FPM), sharing its roots with synthetic aperture technique and phase retrieval method, is a recently developed computational microscopic super-resolution technique. By turning on the light-emitting diode…

Structured illumination has been widely used for optical sectioning and 3D surface recovery. In a typical implementation, multiple images under non-uniform pattern illumination are used to recover a single object section. Axial scanning of…

Versatile, sterically accessible imaging systems capable of in vivo rapid volumetric functional and structural imaging deep in the brain continue to be a limiting factor in neuroscience research. Towards overcoming this obstacle, we present…

FRET-based approaches are a unique tool for sensing the immediate surroundings and interactions of (bio)molecules. FRET imaging and FLIM (Fluorescence Lifetime Imaging Microscopy) enable the visualization of the spatial distribution of…

Confocal microscopy is the backbone of cellular research labs across the world but unfortunately, the imaging is restricted to a single plane. Chromatic confocal microscopy offers the possibility to image multiple planes simultaneously thus…

Real-time 3D fluorescence microscopy is crucial for the spatiotemporal analysis of live organisms, such as neural activity monitoring. The eXtended field-of-view light field microscope (XLFM), also known as Fourier light field microscope,…

We demonstrate numerical refocusing in coherent confocal laser scanning microscopy based on synthetic optical holography. This physics-based approach implements a computational propagation on the complex signal recovered in synthetic…

Calcium imaging permits optical measurement of neural activity. Since intracellular calcium concentration is an indirect measurement of neural activity, computational tools are necessary to infer the true underlying spiking activity from…

A laser Doppler imaging scheme combined to an upright microscope is proposed. Quantitative Doppler imaging in both velocity norm and direction, as well as amplitude contrast of either zebrafish flesh or vasculature is demonstrated.

Deep-tissue optical imaging suffers from the reduction of resolving power due to tissue-induced optical aberrations and multiple scattering noise. Reflection matrix approaches recording the maps of backscattered waves for all the possible…