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Thermal shift assays (TSAs) have been extensively used to study thermodynamics of proteins and provide an efficient means to assess protein-ligand binding or protein-protein interaction. However, existing TSAs have limitations such as time…

We demonstrate millikelvin thermometry of laser cooled trapped ions with high-resolution imaging. This equilibrium approach is independent of the cooling dynamics and has lower systematic error than Doppler thermometry, with \pm5 mK…

量子物理 · 物理学 2011-06-10 B. G. Norton , E. W. Streed , M. J. Petrasiunas , A. Jechow , D. Kielpinski

Proteins can be regarded as thermal nanosensors in an intra-body network. Upon being stimulated by Terahertz (THz) frequencies that match their vibrational modes, protein molecules experience resonant absorption and dissipate their energy…

分子网络 · 定量生物学 2024-07-01 Hadeel Elayan , Samar Elmaadawy , Andrew W. Eckford , Raviraj Adve , Josep Jornet

We review a series of experimental studies of the thermodynamics of nonequilibrium processes at the microscale. In particular, in these experiments we studied the fluctuations of the thermodynamic properties of a single optically-trapped…

统计力学 · 物理学 2016-06-22 L. Dinis , I. A. Martínez , É. Roldán , J. M. R. Parrondo , R. A. Rica

We experimentally demonstrate a method to determine the temperature of trapped ions which is suitable for monitoring fast thermalization processes. We show that observing and analyzing the lineshape of dark resonances in the fluorescence…

Quantifying the temperature of microdevices is critical for probing nanoscale energy transport.Such quantification is often accomplished by integrating resistance thermometers into microdevices. However, such thermometers frequently become…

介观与纳米尺度物理 · 物理学 2021-05-05 Amin Reihani , Shen Yan , Yuxuan Luan , Rohith Mittapally , Edgar Meyhofer , Pramod Reddy

We report on high-temperature sensing measurements using a tubular-lattice hollow-core photonic crystal fiber displaying a microstructure formed of eight 2.4 um-thick cladding tubes. The larger thickness of our fiber's cladding tubes…

Introduced more than fifty years ago, dynamic light scattering is routinely used to determine the size distribution of colloidal suspensions, as well as of macromolecules in solution, such as proteins, nucleic acids, and their complexes.…

ABSTRACT: Temperature monitoring is extremely im-portant during thermotherapy. Fiber-optic temperature sensors are preferred because of their flexibility and im-munity to electromagnetic interference. Although many types of fiber-optic…

医学物理 · 物理学 2020-02-19 Paris L. Franz , Hui Wang

The ability to control and measure the temperature of propagating microwave modes down to very low temperatures is indispensable for quantum information processing, and may open opportunities for studies of heat transport at the nanoscale,…

Temperature sensing is a ubiquitous cell behavior, but the fundamental limits to the precision of temperature sensing are poorly understood. Unlike in chemical concentration sensing, the precision of temperature sensing is not limited by…

生物物理 · 物理学 2021-09-01 Michael Vennettilli , Soutick Saha , Ushasi Roy , Andrew Mugler

Scanning Thermal Microscopy (SThM) has become an important measurement tool for characterizing the thermal properties of materials at the nanometer scale. This technique requires a SThM probe that combines an Atomic Force Microscopy (AFM)…

介观与纳米尺度物理 · 物理学 2024-05-01 R. Swami , G. Julie , S. Le-Denmat , G. Pernot , D. Singhal , J. Paterson , J. Maire , J. F. Motte , N. Paillet , H. Guillou , S. Gomes , O. Bourgeois

Folding and aggregation of proteins, the interaction between proteins and membranes, as well as the adsorption of organic soft matter to inorganic solid substrates belong to the most interesting challenges in understanding structure and…

软凝聚态物质 · 物理学 2007-12-06 Michael Bachmann , Wolfhard Janke

Gas behavior in systems at microscale has been receiving significant attention from researchers in the last two decades [1-4]. Today, there is an enhanced emphasis on developing new experimental techniques to capture the local temperature…

Precision in radiation therapy relies on immobilization systems that limit patient motion. Thermoplastic masks are commonly used for this purpose, but subtle voluntary and involuntary movements such as jaw shifts, deep breathing, or eye…

信号处理 · 电气工程与系统科学 2026-02-03 Ali Kourani , Naveed A. Abbasi , Syeda Narjis Fatima , Katsuyuki Haneda , Andreas F. Molisch

A novel technique for remote, non-contact, in situ monitoring of the protein crystal temperature has been developed for the new I23 beamline at the Diamond Light Source, a facility dedicated to macromolecular crystallography (MX) with…

仪器与探测器 · 物理学 2017-05-16 V. Mykhaylyk A. Wagner , H. Kraus

Microcavity and whispering gallery mode (WGM) biosensors derive their sensitivity from monitoring frequency shifts induced by protein binding at sites of highly confined field intensities, where field strengths can be further amplified by…

Quantum simulations with ultracold atoms typically create atomic wavefunctions with structures at optical length scales, where direct imaging suffers from the diffraction limit. In analogy to advances in optical microscopy for biological…

量子气体 · 物理学 2019-04-10 Sarthak Subhankar , Yang Wang , Tsz-Chun Tsui , Steven L. Rolston , James V. Porto

We report the design and performance of a cryogen-free, pulse-tube refrigerator (PTR)-based scanning probe microscopy (SPM) system capable of operating at the base temperature of near 5K. We achieve this by combining a home-made interface…

材料科学 · 物理学 2022-04-29 Jun Kasai , Tomoki Koyama , Munenori Yokota , Katsuya Iwaya

Temperature estimation plays a vital role across natural sciences. A standard approach is provided by probe thermometry, where a probe is brought into contact with the sample and examined after a certain amount of time has passed. In many…

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