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The molecular chaperone Hsp70 plays a central role in the import of cytoplasmic proteins into organelles, driving their translocation by binding them from the organellar interior. Starting from the experimentally-determined structure of the…

亚细胞过程 · 定量生物学 2014-11-26 Salvatore Assenza , Paolo De Los Rios , Alessandro Barducci

Molecular chaperones are machines that consume copious amount of ATP to drive misfolded proteins or RNA to fold into functionally competent native states. Because the folding landscapes of biomolecules with complex native state topology are…

生物物理 · 物理学 2025-06-17 Changbong Hyeon , D. Thirumalai

Molecular motors drive mechanical motions utilizing the free energy liberated from chemical reactions such as ATP hydrolysis. Although it is essential to know the efficiency of this free energy transduction, it has been a challenge due to…

Chaperonins are biological nanomachines that help newly translated proteins to fold by rescuing them from kinetically trapped misfolded states. Protein folding assistance by the chaperonin machinery is obligatory in vivo for a subset of…

生物大分子 · 定量生物学 2022-11-29 George Stan , George H. Lorimer , D. Thirumalai

Molecular chaperones are ATP-consuming biological machines, which facilitate the folding of proteins and RNA molecules that are kinetically trapped in misfolded states for long times. Unassisted folding occurs by the kinetic partitioning…

生物大分子 · 定量生物学 2019-09-18 D. Thirumalai , George H. Lorimer , Changbong Hyeon

Chaperone-assisted biopolymer translocation is the main model proposed for translocation \textit{in vivo}. A dynamical Monte Carlo method is used to simulate the translocation of a stiff homopolymer through a nanopore driven by chaperones.…

生物物理 · 物理学 2019-12-03 Rouhollh Haji Abdolvahab

Previous kinetic models had assumed that the reaction medium was reacting at random and without a turnover associated to thermodynamics exchanges, with a rigid active site on the enzyme. The experimental studies show that coupling factor 1…

生物大分子 · 定量生物学 2016-11-03 Alfred Bennun

Cytoskeletal filaments are capable of self-assembly in the absence of externally supplied chemical energy, but the rapid turnover rates essential for their biological function require a constant flux of ATP or GTP hydrolysis. The same is…

生物物理 · 物理学 2019-08-26 Robert Marsland , Jeremy England

Molecular motors fulfill critical functions within all living beings. Understanding their underlying working principles is therefore of great interest. Here we develop a simple model inspired by the two-component biomolecular motor Fo-F1…

统计力学 · 物理学 2024-12-30 Mathis Grelier , David A. Sivak , Jannik Ehrich

The cytoskeletal component actomyosin is a canonical example of active matter since the powerstroke cycle locally converts chemical energy in the form of adenoside triphosphate (ATP) into mechanical work for remodelling. Observing myosin II…

Many biological functions are executed by molecular machines, which consume energy and convert it into mechanical work. Biological machines have evolved to transport cargo, facilitate folding of proteins and RNA, remodel chromatin and…

生物物理 · 物理学 2020-04-15 Mauro L. Mugnai , Changbong Hyeon , Michael Hinczewski , D. Thirumalai

The enzyme FoF1-ATP synthase provides the 'chemical energy currency' adenosine triphosphate (ATP) for living cells. Catalysis is driven by mechanochemical coupling of subunit rotation within the enzyme with conformational changes in the…

生物大分子 · 定量生物学 2015-06-04 Stefan Ernst , Monika G. Dueser , Nawid Zarrabi , Michael Boersch

When a system is in equilibrium, external perturbations yield a time series of non-equilibrium distributions, and recent experimental techniques give access to the non-equilibrium data that may contain critical information. Jinwoo and…

统计力学 · 物理学 2023-01-16 Lee Jinwoo

We study a model of synthetic molecular motor - a [3]-catenane consisting of two small macrocycles mechanically interlocked with a bigger one - subjected to a time-dependent driving using stochastic thermodynamics. The model presents…

统计力学 · 物理学 2023-06-28 Massimo Bilancioni , Massimiliano Esposito , Emanuele Penocchio

Biomolecular machines consume free energy to break symmetry and make directed progress. Nonequilibrium ATP concentrations are the typical free energy source, with one cycle of a molecular machine consuming a certain number of ATP, providing…

统计力学 · 物理学 2017-10-23 Aidan I Brown , David A Sivak

F$_{1}$-ATPase is a rotary molecular motor that \emph{in vivo} is subject to strong nonequilibrium driving forces. There is great interest in understanding the operational principles governing its high efficiency of free-energy…

统计力学 · 物理学 2022-12-29 Deepak Gupta , Steven J. Large , Shoichi Toyabe , David A. Sivak

FoF1-ATP synthase is the enzyme that provides the 'chemical energy currency' adenosine triphosphate, ATP, for living cells. The formation of ATP is accomplished by a stepwise internal rotation of subunits within the enzyme. We monitor…

生物物理 · 物理学 2015-06-26 N. Zarrabi , M. G. Dueser , R. Reuter , S. D. Dunn , J. Wrachtrup , M. Boersch

As a consequence of the rugged landscape of RNA molecules their folding is described by the kinetic partitioning mechanism according to which only a small fraction ($\phi_F$) reaches the folded state while the remaining fraction of…

生物大分子 · 定量生物学 2017-01-24 Changbong Hyeon , D. Thirumalai

Molecular machines composed of coupled subsystems transduce free energy between different external reservoirs, in the process internally transducing energy and information. While subsystem efficiencies of these molecular machines have been…

统计力学 · 物理学 2023-05-23 Matthew P. Leighton , David A. Sivak

Active networks made of biopolymers and motor proteins are valuable bioinspired systems that have been used in the last decades to study the cytoskeleton and its self-organization under mechanical stimulation. Different techniques are…

软凝聚态物质 · 物理学 2023-05-15 Vahid Nasirimarekani , Olinka Ramìrez-Soto , Stefan Karpitschka , Isabella Guido
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