相关论文: Calibrating evanescent-wave penetration depths for…
Total internal reflection fluorescence (TIRF) microscopy and its variants are key technologies for visualizing the dynamics of single molecules or organelles in live cells. Yet, truely quantitative TIRF remains problematic. One unknown…
Evanescent-wave microscopy achieves sub-diffraction axial sectioning by confining fluorescence excitation to a thin layer close to the cell/substrate interface. How thin this light sheet exactly is, however, is often unknown. Particularly…
Describing the phenomenon of total internal reflection in terms of a reflection coefficient of unit magnitude, we found that, not only can propagating plane waves be total internally reflected at the planar interface of two dissimilar,…
Paradoxically, imaging with resolution much below the wavelength $\lambda$ - now common place in the visible spectrum - remains challenging at lower frequencies, where arguably it is needed most due to the large wavelengths used. Techniques…
The resolving ability of widefield fluorescence microscopy is fundamentally limited by out-of-focus background owing to its low axial resolution, particularly for densely labeled biological samples. Although total internal reflection…
Multi-dielectric coated coverslip can be designed to reach large optical field enhancements when working under proper illumination conditions and in total internal reflection. In an objective-based total internal reflection fluorescence…
Total internal reflection (TIR) is a ubiquitous phenomenon used in photonic devices ranging from waveguides and resonators to lasers and optical sensors. Controlling this phenomenon and light confinement are keys to the future integration…
For measurements designed to accurately determine layer thickness, there is a natural trade-off between sensitivity to optical thickness and lateral resolution due to the angular ray distribution required for a focused beam. We demonstrate…
We propose and demonstrate, through simulation and experiment, how the interaction of an optical field within a waveguide designed for chemical sensing and, more generally, evanescent field spectroscopy can be enhanced substantially by…
Evanescent waves are central to many technologies such as near-field imaging that beats the diffraction limit and plasmonic devices. Frustrated total internal reflection (FTIR) is an experimental method commonly used to study evanescent…
Total internal reflection fluorescence microscopy (TIRF) has enabled low-background, live-cell friendly imaging of cell surfaces and other thin samples thanks to the shallow penetration of the evanescent light field into the sample. The…
Total Internal Reflection Microscopy (TIRM) is a sensitive non-invasive technique to measure the interaction potentials between a colloidal particle and a wall with femtonewton resolution. The equilibrium distribution of the particle-wall…
Frustrated total internal reflection is analyzed from an unusual point of view Unlike most similar works, incident angles are used here as the scanning variable, instead of the tunneled film thickness. The theoretical framework is presented…
The present study deals with total internal reflection of a plane electromagnetic wave at an infinite plane boundary between a transparent medium and an amplifying or attenuating lower-index medium. Solutions of Maxwell's equations are…
We observe and investigate, both experimentally and theoretically, electromagnetically-induced transparency experienced by evanescent fields arising due to total internal reflection from an interface of glass and hot rubidium vapor. This…
Fluorescence microscopy is widely used in biological imaging, however scattering from tissues strongly limits its applicability to a shallow depth. In this work we adapt a methodology inspired from stellar speckle interferometry, and…
Protein function is inherently linked to its localization within the cell, and fluorescent microscopy data is an indispensable resource for learning representations of proteins. Despite major developments in molecular representation…
Molecular interactions are key to many chemical and biological processes like protein function. In many signaling processes they occur in sub-cellular areas displaying nanoscale organizations and involving molecular assemblies. The…
Fluorescence detection, either involving propagating or near-field emission, is widely being used in spectroscopy, sensing and microscopy. Total internal reflection fluorescence (TIRF) confines fluorescence excitation by an evanescent…
In biological microscopy, light scattering represents the main limitation to image at depth. Recently, a set of wavefront shaping techniques has been developed in order to manipulate coherent light in strongly disordered materials. The…