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Increasing evidence suggests that chromosome folding and genetic expression are intimately connected. For example, the co-expression of a large number of genes can benefit from their spatial co-localization in the cellular space.…

软凝聚态物质 · 物理学 2018-03-13 Guillaume Le Treut

Three-dimensional (3D) chromatin structure is closely related to genome function, in particular transcription. However, the folding path of the chromatin fiber in the interphase nucleus is unknown. Here, we systematically measured the 3D…

The three-dimensional organisation of chromosomes can be probed using methods such as Capture-C. However it is unclear how such population level data relates to the organisation within a single cell, and the mechanisms leading to the…

The probability of two loci, separated by a certain genome length, being in contact can be inferred using the Chromosome Conformation Capture (3C) method and related Hi-C experiments. How to go from the contact map, a matrix listing the…

软凝聚态物质 · 物理学 2021-03-24 Guang Shi , D. Thirumalai

The three-dimensional organization of chromatin, on the length scale of a few genes, is crucial in determining the functional state - accessibility and the amount of gene expression - of the chromatin. Recent advances in chromosome…

软凝聚态物质 · 物理学 2021-07-16 K. Kumari , B. Duenweg , R. Padinhateeri , J. Ravi Prakash

The 3-dimensional (3D) structure of the genome is of significant importance for many cellular processes. In this paper, we study the problem of reconstructing the 3D structure of chromosomes from Hi-C data of diploid organisms, which poses…

基因组学 · 定量生物学 2024-05-30 Diego Cifuentes , Jan Draisma , Oskar Henriksson , Annachiara Korchmaros , Kaie Kubjas

How long threadlike eukaryotic chromosomes fit tidily in the small volume of the nucleus without significant entanglement is just beginning to be understood, thanks to major advances in experimental techniques. Several polymer models, which…

软凝聚态物质 · 物理学 2024-10-03 D. Thirumalai , Guang Shi , Sucheol Shin , Changbong Hyeon

The three dimensional structure of DNA in the nucleus (chromatin) plays an important role in many cellular processes. Recent experimental advances have led to high-throughput methods of capturing information about chromatin conformation on…

基因组学 · 定量生物学 2015-11-05 Kevin Emmett , Benjamin Schweinhart , Raul Rabadan

Chromosome organisation is increasingly recognised as an essential component of genome regulation, cell fate and cell health. Within the realm of transposable elements (TEs) however, the spatial information of how genomes are folded is…

生物物理 · 物理学 2019-11-01 Alexandros Bousios , Hans-Wilhelm Nuetzmann , Dorothy Buck , Davide Michieletto

Combining genome-wide structural models with phenomenological data is at the forefront of efforts to understand the organizational principles regulating the human genome. Here, we use chromosome-chromosome contact data as knowledge-based…

生物物理 · 物理学 2016-10-31 Marco Di Stefano , Jonas Paulsen , Tonje G. Lien , Eivind Hovig , Cristian Micheletti

The statistical properties of intra-chromosome maps obtained by a genome-wide chromosome conformation capture method (Hi-C) are described in the framework of the hierarchical crumpling model of heteropolymer chain with quenched disorder in…

基因组学 · 定量生物学 2013-12-02 L. Nazarov , M. V. Tamm , S. K. Nechaev , V. A. Avetisov

We propose a polymer model for the dynamics of chromatin replication in three dimensional space. Our simulations indicate that both immobile and tracking replisomes may self-assemble during the process, reconciling previous apparently…

生物物理 · 物理学 2023-12-19 G. Forte , S. Buonomo , P. R. Cook , N. Gilbert , D. Marenduzzo , E. Orlandini

Cellular functions crucially depend on the precise execution of complex biochemical reactions taking place on the chromatin fiber in the tightly packed environment of the cell nucleus. Despite the availability of large data sets probing…

定量方法 · 定量生物学 2023-03-17 Alex Chen Yi Zhang , Angelo Rosa , Guido Sanguinetti

We present a minimal computational model, which allows very fast, on-the-fly construction of three dimensional haploid interphase genomes from single cell Hi-C contact maps using the HOOMD-blue molecular dynamics package on graphics…

软凝聚态物质 · 物理学 2019-11-14 S. Wettermann , M. Brems , J. T. Siebert , G. T. Vu , T. J. Stevens , P. Virnau

Protein-mediated interactions are ubiquitous in the cellular environment, and particularly in the nucleus, where they are responsible for the structuring of chromatin. We show through molecular--dynamics simulations of a polymer surrounded…

生物大分子 · 定量生物学 2024-03-21 Francesco Borando , Guido Tiana

The three-dimensional organization of chromatin is thought to play an important role in controlling gene expression. Specificity in expression is achieved through the interaction of transcription factors and other nuclear proteins with…

生物物理 · 物理学 2025-07-22 Mason N. Rouches , Benjamin B. Machta

The spatial organization of chromatin within the nucleus plays a crucial role in gene expression and genome function. However, the quantitative relationship between this organization and nuclear biochemical processes remains under debate.…

定量方法 · 定量生物学 2025-09-12 Alex Chen Yi Zhang , Angelo Rosa , Guido Sanguinetti

Understanding the three-dimensional (3D) structure of the genome is essential for elucidating vital biological processes and their links to human disease. To determine how the genome folds within the nucleus, chromosome conformation capture…

The 3D folding of a mammalian gene can be studied by a polymer model, where the chromatin fibre is represented by a semiflexible polymer which interacts with multivalent proteins, representing complexes of DNA-binding transcription factors…

The chromatin folding and the spatial arrangement of chromosomes in the cell play a crucial role in DNA replication and genes expression. An improper chromatin folding could lead to malfunctions and, over time, diseases. For eukaryotes,…

机器学习 · 统计学 2025-11-13 Eloïse Touron , Pedro L. C. Rodrigues , Julyan Arbel , Nelle Varoquaux , Michael Arbel
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